Job ID = 14521501 SRX = SRX10754399 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7596086 spots for SRR14402206/SRR14402206.sra Written 7596086 spots for SRR14402206/SRR14402206.sra Read 7208403 spots for SRR14402207/SRR14402207.sra Written 7208403 spots for SRR14402207/SRR14402207.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:02 14804489 reads; of these: 14804489 (100.00%) were paired; of these: 539182 (3.64%) aligned concordantly 0 times 12146773 (82.05%) aligned concordantly exactly 1 time 2118534 (14.31%) aligned concordantly >1 times ---- 539182 pairs aligned concordantly 0 times; of these: 129849 (24.08%) aligned discordantly 1 time ---- 409333 pairs aligned 0 times concordantly or discordantly; of these: 818666 mates make up the pairs; of these: 629998 (76.95%) aligned 0 times 103063 (12.59%) aligned exactly 1 time 85605 (10.46%) aligned >1 times 97.87% overall alignment rate Time searching: 00:09:02 Overall time: 00:09:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2267536 / 14333824 = 0.1582 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:22:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:22:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:22:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:22:50: 1000000 INFO @ Sat, 15 Jan 2022 21:22:54: 2000000 INFO @ Sat, 15 Jan 2022 21:22:58: 3000000 INFO @ Sat, 15 Jan 2022 21:23:03: 4000000 INFO @ Sat, 15 Jan 2022 21:23:07: 5000000 INFO @ Sat, 15 Jan 2022 21:23:11: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:23:15: 7000000 INFO @ Sat, 15 Jan 2022 21:23:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:23:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:23:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:23:20: 8000000 INFO @ Sat, 15 Jan 2022 21:23:20: 1000000 INFO @ Sat, 15 Jan 2022 21:23:24: 9000000 INFO @ Sat, 15 Jan 2022 21:23:25: 2000000 INFO @ Sat, 15 Jan 2022 21:23:29: 10000000 INFO @ Sat, 15 Jan 2022 21:23:29: 3000000 INFO @ Sat, 15 Jan 2022 21:23:33: 11000000 INFO @ Sat, 15 Jan 2022 21:23:34: 4000000 INFO @ Sat, 15 Jan 2022 21:23:37: 12000000 INFO @ Sat, 15 Jan 2022 21:23:38: 5000000 INFO @ Sat, 15 Jan 2022 21:23:42: 13000000 INFO @ Sat, 15 Jan 2022 21:23:43: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:23:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:23:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:23:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:23:46: 14000000 INFO @ Sat, 15 Jan 2022 21:23:47: 7000000 INFO @ Sat, 15 Jan 2022 21:23:50: 1000000 INFO @ Sat, 15 Jan 2022 21:23:51: 15000000 INFO @ Sat, 15 Jan 2022 21:23:52: 8000000 INFO @ Sat, 15 Jan 2022 21:23:55: 2000000 INFO @ Sat, 15 Jan 2022 21:23:55: 16000000 INFO @ Sat, 15 Jan 2022 21:23:56: 9000000 INFO @ Sat, 15 Jan 2022 21:23:59: 3000000 INFO @ Sat, 15 Jan 2022 21:24:00: 17000000 INFO @ Sat, 15 Jan 2022 21:24:01: 10000000 INFO @ Sat, 15 Jan 2022 21:24:04: 4000000 INFO @ Sat, 15 Jan 2022 21:24:04: 18000000 INFO @ Sat, 15 Jan 2022 21:24:05: 11000000 INFO @ Sat, 15 Jan 2022 21:24:08: 5000000 INFO @ Sat, 15 Jan 2022 21:24:09: 19000000 INFO @ Sat, 15 Jan 2022 21:24:10: 12000000 INFO @ Sat, 15 Jan 2022 21:24:13: 6000000 INFO @ Sat, 15 Jan 2022 21:24:13: 20000000 INFO @ Sat, 15 Jan 2022 21:24:14: 13000000 INFO @ Sat, 15 Jan 2022 21:24:17: 7000000 INFO @ Sat, 15 Jan 2022 21:24:18: 21000000 INFO @ Sat, 15 Jan 2022 21:24:19: 14000000 INFO @ Sat, 15 Jan 2022 21:24:22: 8000000 INFO @ Sat, 15 Jan 2022 21:24:22: 22000000 INFO @ Sat, 15 Jan 2022 21:24:23: 15000000 INFO @ Sat, 15 Jan 2022 21:24:26: 9000000 INFO @ Sat, 15 Jan 2022 21:24:26: 23000000 INFO @ Sat, 15 Jan 2022 21:24:27: 16000000 INFO @ Sat, 15 Jan 2022 21:24:31: 10000000 INFO @ Sat, 15 Jan 2022 21:24:31: 24000000 INFO @ Sat, 15 Jan 2022 21:24:32: 17000000 INFO @ Sat, 15 Jan 2022 21:24:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:24:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:24:33: #1 total tags in treatment: 12012102 INFO @ Sat, 15 Jan 2022 21:24:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:24:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:24:33: #1 tags after filtering in treatment: 8475431 INFO @ Sat, 15 Jan 2022 21:24:33: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:24:33: #1 finished! INFO @ Sat, 15 Jan 2022 21:24:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:24:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:24:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:24:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:24:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:24:35: 11000000 INFO @ Sat, 15 Jan 2022 21:24:37: 18000000 INFO @ Sat, 15 Jan 2022 21:24:40: 12000000 INFO @ Sat, 15 Jan 2022 21:24:41: 19000000 INFO @ Sat, 15 Jan 2022 21:24:44: 13000000 INFO @ Sat, 15 Jan 2022 21:24:46: 20000000 INFO @ Sat, 15 Jan 2022 21:24:49: 14000000 INFO @ Sat, 15 Jan 2022 21:24:50: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:24:53: 15000000 INFO @ Sat, 15 Jan 2022 21:24:54: 22000000 INFO @ Sat, 15 Jan 2022 21:24:58: 16000000 INFO @ Sat, 15 Jan 2022 21:24:59: 23000000 INFO @ Sat, 15 Jan 2022 21:25:02: 17000000 INFO @ Sat, 15 Jan 2022 21:25:03: 24000000 INFO @ Sat, 15 Jan 2022 21:25:05: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:25:05: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:25:05: #1 total tags in treatment: 12012102 INFO @ Sat, 15 Jan 2022 21:25:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:25:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:25:05: #1 tags after filtering in treatment: 8475431 INFO @ Sat, 15 Jan 2022 21:25:05: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:25:05: #1 finished! INFO @ Sat, 15 Jan 2022 21:25:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:25:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:25:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:25:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:25:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:25:07: 18000000 INFO @ Sat, 15 Jan 2022 21:25:11: 19000000 INFO @ Sat, 15 Jan 2022 21:25:15: 20000000 INFO @ Sat, 15 Jan 2022 21:25:19: 21000000 INFO @ Sat, 15 Jan 2022 21:25:23: 22000000 INFO @ Sat, 15 Jan 2022 21:25:28: 23000000 INFO @ Sat, 15 Jan 2022 21:25:32: 24000000 INFO @ Sat, 15 Jan 2022 21:25:34: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:25:34: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:25:34: #1 total tags in treatment: 12012102 INFO @ Sat, 15 Jan 2022 21:25:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:25:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:25:34: #1 tags after filtering in treatment: 8475431 INFO @ Sat, 15 Jan 2022 21:25:34: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:25:34: #1 finished! INFO @ Sat, 15 Jan 2022 21:25:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:25:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:25:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:25:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:25:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754399/SRX10754399.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling