Job ID = 14521499 SRX = SRX10754397 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6668218 spots for SRR14402202/SRR14402202.sra Written 6668218 spots for SRR14402202/SRR14402202.sra Read 6372816 spots for SRR14402203/SRR14402203.sra Written 6372816 spots for SRR14402203/SRR14402203.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:57 13041034 reads; of these: 13041034 (100.00%) were paired; of these: 572942 (4.39%) aligned concordantly 0 times 10192153 (78.15%) aligned concordantly exactly 1 time 2275939 (17.45%) aligned concordantly >1 times ---- 572942 pairs aligned concordantly 0 times; of these: 98201 (17.14%) aligned discordantly 1 time ---- 474741 pairs aligned 0 times concordantly or discordantly; of these: 949482 mates make up the pairs; of these: 785881 (82.77%) aligned 0 times 81836 (8.62%) aligned exactly 1 time 81765 (8.61%) aligned >1 times 96.99% overall alignment rate Time searching: 00:09:57 Overall time: 00:09:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2184166 / 12522042 = 0.1744 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:22:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:22:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:22:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:22:28: 1000000 INFO @ Sat, 15 Jan 2022 21:22:34: 2000000 INFO @ Sat, 15 Jan 2022 21:22:40: 3000000 INFO @ Sat, 15 Jan 2022 21:22:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:22:51: 5000000 INFO @ Sat, 15 Jan 2022 21:22:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:22:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:22:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:22:58: 6000000 INFO @ Sat, 15 Jan 2022 21:22:59: 1000000 INFO @ Sat, 15 Jan 2022 21:23:04: 7000000 INFO @ Sat, 15 Jan 2022 21:23:05: 2000000 INFO @ Sat, 15 Jan 2022 21:23:11: 8000000 INFO @ Sat, 15 Jan 2022 21:23:12: 3000000 INFO @ Sat, 15 Jan 2022 21:23:17: 9000000 INFO @ Sat, 15 Jan 2022 21:23:18: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:23:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:23:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:23:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:23:24: 10000000 INFO @ Sat, 15 Jan 2022 21:23:25: 5000000 INFO @ Sat, 15 Jan 2022 21:23:29: 1000000 INFO @ Sat, 15 Jan 2022 21:23:30: 11000000 INFO @ Sat, 15 Jan 2022 21:23:31: 6000000 INFO @ Sat, 15 Jan 2022 21:23:36: 2000000 INFO @ Sat, 15 Jan 2022 21:23:37: 12000000 INFO @ Sat, 15 Jan 2022 21:23:38: 7000000 INFO @ Sat, 15 Jan 2022 21:23:43: 3000000 INFO @ Sat, 15 Jan 2022 21:23:44: 13000000 INFO @ Sat, 15 Jan 2022 21:23:46: 8000000 INFO @ Sat, 15 Jan 2022 21:23:50: 4000000 INFO @ Sat, 15 Jan 2022 21:23:51: 14000000 INFO @ Sat, 15 Jan 2022 21:23:53: 9000000 INFO @ Sat, 15 Jan 2022 21:23:57: 5000000 INFO @ Sat, 15 Jan 2022 21:23:58: 15000000 INFO @ Sat, 15 Jan 2022 21:24:00: 10000000 INFO @ Sat, 15 Jan 2022 21:24:04: 6000000 INFO @ Sat, 15 Jan 2022 21:24:05: 16000000 INFO @ Sat, 15 Jan 2022 21:24:07: 11000000 INFO @ Sat, 15 Jan 2022 21:24:11: 7000000 INFO @ Sat, 15 Jan 2022 21:24:12: 17000000 INFO @ Sat, 15 Jan 2022 21:24:14: 12000000 INFO @ Sat, 15 Jan 2022 21:24:18: 8000000 INFO @ Sat, 15 Jan 2022 21:24:19: 18000000 INFO @ Sat, 15 Jan 2022 21:24:20: 13000000 INFO @ Sat, 15 Jan 2022 21:24:26: 9000000 INFO @ Sat, 15 Jan 2022 21:24:26: 19000000 INFO @ Sat, 15 Jan 2022 21:24:27: 14000000 INFO @ Sat, 15 Jan 2022 21:24:33: 10000000 INFO @ Sat, 15 Jan 2022 21:24:33: 20000000 INFO @ Sat, 15 Jan 2022 21:24:34: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:24:39: 11000000 INFO @ Sat, 15 Jan 2022 21:24:39: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:24:39: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:24:39: #1 total tags in treatment: 10295937 INFO @ Sat, 15 Jan 2022 21:24:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:24:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:24:40: #1 tags after filtering in treatment: 7304135 INFO @ Sat, 15 Jan 2022 21:24:40: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:24:40: #1 finished! INFO @ Sat, 15 Jan 2022 21:24:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:24:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:24:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:24:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:24:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:24:40: 16000000 INFO @ Sat, 15 Jan 2022 21:24:46: 12000000 INFO @ Sat, 15 Jan 2022 21:24:47: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:24:52: 13000000 INFO @ Sat, 15 Jan 2022 21:24:53: 18000000 INFO @ Sat, 15 Jan 2022 21:24:58: 14000000 INFO @ Sat, 15 Jan 2022 21:25:00: 19000000 INFO @ Sat, 15 Jan 2022 21:25:04: 15000000 INFO @ Sat, 15 Jan 2022 21:25:06: 20000000 INFO @ Sat, 15 Jan 2022 21:25:11: 16000000 INFO @ Sat, 15 Jan 2022 21:25:12: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:25:12: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:25:12: #1 total tags in treatment: 10295937 INFO @ Sat, 15 Jan 2022 21:25:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:25:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:25:12: #1 tags after filtering in treatment: 7304135 INFO @ Sat, 15 Jan 2022 21:25:12: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:25:12: #1 finished! INFO @ Sat, 15 Jan 2022 21:25:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:25:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:25:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:25:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:25:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:25:16: 17000000 INFO @ Sat, 15 Jan 2022 21:25:22: 18000000 INFO @ Sat, 15 Jan 2022 21:25:28: 19000000 INFO @ Sat, 15 Jan 2022 21:25:34: 20000000 INFO @ Sat, 15 Jan 2022 21:25:39: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:25:39: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:25:39: #1 total tags in treatment: 10295937 INFO @ Sat, 15 Jan 2022 21:25:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:25:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:25:39: #1 tags after filtering in treatment: 7304135 INFO @ Sat, 15 Jan 2022 21:25:39: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:25:39: #1 finished! INFO @ Sat, 15 Jan 2022 21:25:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:25:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:25:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:25:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:25:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754397/SRX10754397.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling