Job ID = 14521497 SRX = SRX10754395 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8851767 spots for SRR14402198/SRR14402198.sra Written 8851767 spots for SRR14402198/SRR14402198.sra Read 8364145 spots for SRR14402199/SRR14402199.sra Written 8364145 spots for SRR14402199/SRR14402199.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:32 17215912 reads; of these: 17215912 (100.00%) were paired; of these: 724434 (4.21%) aligned concordantly 0 times 14442953 (83.89%) aligned concordantly exactly 1 time 2048525 (11.90%) aligned concordantly >1 times ---- 724434 pairs aligned concordantly 0 times; of these: 157662 (21.76%) aligned discordantly 1 time ---- 566772 pairs aligned 0 times concordantly or discordantly; of these: 1133544 mates make up the pairs; of these: 904741 (79.82%) aligned 0 times 146327 (12.91%) aligned exactly 1 time 82476 (7.28%) aligned >1 times 97.37% overall alignment rate Time searching: 00:09:32 Overall time: 00:09:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3786835 / 16593060 = 0.2282 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:33: 1000000 INFO @ Sat, 15 Jan 2022 21:29:38: 2000000 INFO @ Sat, 15 Jan 2022 21:29:43: 3000000 INFO @ Sat, 15 Jan 2022 21:29:48: 4000000 INFO @ Sat, 15 Jan 2022 21:29:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:59: 6000000 INFO @ Sat, 15 Jan 2022 21:30:05: 1000000 INFO @ Sat, 15 Jan 2022 21:30:06: 7000000 INFO @ Sat, 15 Jan 2022 21:30:12: 8000000 INFO @ Sat, 15 Jan 2022 21:30:13: 2000000 INFO @ Sat, 15 Jan 2022 21:30:19: 9000000 INFO @ Sat, 15 Jan 2022 21:30:21: 3000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 21:30:26: 10000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:30:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:30:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:30:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:30:29: 4000000 INFO @ Sat, 15 Jan 2022 21:30:33: 11000000 INFO @ Sat, 15 Jan 2022 21:30:35: 1000000 INFO @ Sat, 15 Jan 2022 21:30:37: 5000000 INFO @ Sat, 15 Jan 2022 21:30:40: 12000000 INFO @ Sat, 15 Jan 2022 21:30:42: 2000000 INFO @ Sat, 15 Jan 2022 21:30:45: 6000000 INFO @ Sat, 15 Jan 2022 21:30:46: 13000000 INFO @ Sat, 15 Jan 2022 21:30:50: 3000000 INFO @ Sat, 15 Jan 2022 21:30:53: 7000000 INFO @ Sat, 15 Jan 2022 21:30:53: 14000000 INFO @ Sat, 15 Jan 2022 21:30:58: 4000000 INFO @ Sat, 15 Jan 2022 21:31:00: 8000000 INFO @ Sat, 15 Jan 2022 21:31:00: 15000000 INFO @ Sat, 15 Jan 2022 21:31:05: 5000000 INFO @ Sat, 15 Jan 2022 21:31:07: 16000000 INFO @ Sat, 15 Jan 2022 21:31:07: 9000000 INFO @ Sat, 15 Jan 2022 21:31:13: 6000000 INFO @ Sat, 15 Jan 2022 21:31:14: 17000000 INFO @ Sat, 15 Jan 2022 21:31:14: 10000000 INFO @ Sat, 15 Jan 2022 21:31:20: 7000000 INFO @ Sat, 15 Jan 2022 21:31:21: 18000000 INFO @ Sat, 15 Jan 2022 21:31:22: 11000000 INFO @ Sat, 15 Jan 2022 21:31:28: 8000000 INFO @ Sat, 15 Jan 2022 21:31:28: 19000000 INFO @ Sat, 15 Jan 2022 21:31:29: 12000000 INFO @ Sat, 15 Jan 2022 21:31:34: 20000000 INFO @ Sat, 15 Jan 2022 21:31:35: 9000000 INFO @ Sat, 15 Jan 2022 21:31:36: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:31:41: 21000000 INFO @ Sat, 15 Jan 2022 21:31:42: 10000000 INFO @ Sat, 15 Jan 2022 21:31:44: 14000000 INFO @ Sat, 15 Jan 2022 21:31:48: 22000000 INFO @ Sat, 15 Jan 2022 21:31:50: 11000000 INFO @ Sat, 15 Jan 2022 21:31:51: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:31:55: 23000000 INFO @ Sat, 15 Jan 2022 21:31:57: 12000000 INFO @ Sat, 15 Jan 2022 21:31:59: 16000000 INFO @ Sat, 15 Jan 2022 21:32:02: 24000000 INFO @ Sat, 15 Jan 2022 21:32:05: 13000000 INFO @ Sat, 15 Jan 2022 21:32:07: 17000000 INFO @ Sat, 15 Jan 2022 21:32:08: 25000000 INFO @ Sat, 15 Jan 2022 21:32:12: 14000000 INFO @ Sat, 15 Jan 2022 21:32:14: 18000000 INFO @ Sat, 15 Jan 2022 21:32:15: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:32:15: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:32:15: #1 total tags in treatment: 12732032 INFO @ Sat, 15 Jan 2022 21:32:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:32:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:32:15: #1 tags after filtering in treatment: 6399897 INFO @ Sat, 15 Jan 2022 21:32:15: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 21:32:15: #1 finished! INFO @ Sat, 15 Jan 2022 21:32:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:32:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:32:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:32:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:32:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:32:20: 15000000 INFO @ Sat, 15 Jan 2022 21:32:22: 19000000 INFO @ Sat, 15 Jan 2022 21:32:28: 16000000 INFO @ Sat, 15 Jan 2022 21:32:30: 20000000 INFO @ Sat, 15 Jan 2022 21:32:35: 17000000 INFO @ Sat, 15 Jan 2022 21:32:37: 21000000 INFO @ Sat, 15 Jan 2022 21:32:42: 18000000 INFO @ Sat, 15 Jan 2022 21:32:44: 22000000 INFO @ Sat, 15 Jan 2022 21:32:50: 19000000 INFO @ Sat, 15 Jan 2022 21:32:51: 23000000 INFO @ Sat, 15 Jan 2022 21:32:57: 20000000 INFO @ Sat, 15 Jan 2022 21:32:58: 24000000 INFO @ Sat, 15 Jan 2022 21:33:04: 21000000 INFO @ Sat, 15 Jan 2022 21:33:05: 25000000 INFO @ Sat, 15 Jan 2022 21:33:11: 22000000 INFO @ Sat, 15 Jan 2022 21:33:12: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:33:12: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:33:12: #1 total tags in treatment: 12732032 INFO @ Sat, 15 Jan 2022 21:33:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:33:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:33:12: #1 tags after filtering in treatment: 6399897 INFO @ Sat, 15 Jan 2022 21:33:12: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 21:33:12: #1 finished! INFO @ Sat, 15 Jan 2022 21:33:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:33:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:33:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:33:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:33:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:33:17: 23000000 INFO @ Sat, 15 Jan 2022 21:33:24: 24000000 INFO @ Sat, 15 Jan 2022 21:33:30: 25000000 INFO @ Sat, 15 Jan 2022 21:33:36: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:33:36: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:33:36: #1 total tags in treatment: 12732032 INFO @ Sat, 15 Jan 2022 21:33:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:33:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:33:36: #1 tags after filtering in treatment: 6399897 INFO @ Sat, 15 Jan 2022 21:33:36: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 15 Jan 2022 21:33:36: #1 finished! INFO @ Sat, 15 Jan 2022 21:33:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:33:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:33:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:33:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:33:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754395/SRX10754395.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling