Job ID = 14521495 SRX = SRX10754393 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8734387 spots for SRR14402194/SRR14402194.sra Written 8734387 spots for SRR14402194/SRR14402194.sra Read 8313440 spots for SRR14402195/SRR14402195.sra Written 8313440 spots for SRR14402195/SRR14402195.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:04 17047827 reads; of these: 17047827 (100.00%) were paired; of these: 777050 (4.56%) aligned concordantly 0 times 13873472 (81.38%) aligned concordantly exactly 1 time 2397305 (14.06%) aligned concordantly >1 times ---- 777050 pairs aligned concordantly 0 times; of these: 231630 (29.81%) aligned discordantly 1 time ---- 545420 pairs aligned 0 times concordantly or discordantly; of these: 1090840 mates make up the pairs; of these: 817028 (74.90%) aligned 0 times 166657 (15.28%) aligned exactly 1 time 107155 (9.82%) aligned >1 times 97.60% overall alignment rate Time searching: 00:13:05 Overall time: 00:13:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3418111 / 16457077 = 0.2077 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:50:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:50:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:50:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:50:42: 1000000 INFO @ Sat, 15 Jan 2022 21:50:48: 2000000 INFO @ Sat, 15 Jan 2022 21:50:54: 3000000 INFO @ Sat, 15 Jan 2022 21:51:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:51:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:51:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:51:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:51:07: 5000000 INFO @ Sat, 15 Jan 2022 21:51:13: 1000000 INFO @ Sat, 15 Jan 2022 21:51:13: 6000000 INFO @ Sat, 15 Jan 2022 21:51:19: 2000000 INFO @ Sat, 15 Jan 2022 21:51:20: 7000000 INFO @ Sat, 15 Jan 2022 21:51:26: 3000000 INFO @ Sat, 15 Jan 2022 21:51:26: 8000000 INFO @ Sat, 15 Jan 2022 21:51:33: 4000000 INFO @ Sat, 15 Jan 2022 21:51:33: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:51:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:51:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:51:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:51:39: 5000000 INFO @ Sat, 15 Jan 2022 21:51:40: 10000000 INFO @ Sat, 15 Jan 2022 21:51:43: 1000000 INFO @ Sat, 15 Jan 2022 21:51:46: 6000000 INFO @ Sat, 15 Jan 2022 21:51:47: 11000000 INFO @ Sat, 15 Jan 2022 21:51:51: 2000000 INFO @ Sat, 15 Jan 2022 21:51:53: 7000000 INFO @ Sat, 15 Jan 2022 21:51:54: 12000000 INFO @ Sat, 15 Jan 2022 21:51:58: 3000000 INFO @ Sat, 15 Jan 2022 21:52:00: 8000000 INFO @ Sat, 15 Jan 2022 21:52:02: 13000000 INFO @ Sat, 15 Jan 2022 21:52:05: 4000000 INFO @ Sat, 15 Jan 2022 21:52:07: 9000000 INFO @ Sat, 15 Jan 2022 21:52:09: 14000000 INFO @ Sat, 15 Jan 2022 21:52:12: 5000000 INFO @ Sat, 15 Jan 2022 21:52:15: 10000000 INFO @ Sat, 15 Jan 2022 21:52:16: 15000000 INFO @ Sat, 15 Jan 2022 21:52:20: 6000000 INFO @ Sat, 15 Jan 2022 21:52:23: 11000000 INFO @ Sat, 15 Jan 2022 21:52:24: 16000000 INFO @ Sat, 15 Jan 2022 21:52:28: 7000000 INFO @ Sat, 15 Jan 2022 21:52:30: 12000000 INFO @ Sat, 15 Jan 2022 21:52:32: 17000000 INFO @ Sat, 15 Jan 2022 21:52:36: 8000000 INFO @ Sat, 15 Jan 2022 21:52:38: 13000000 INFO @ Sat, 15 Jan 2022 21:52:40: 18000000 INFO @ Sat, 15 Jan 2022 21:52:44: 9000000 INFO @ Sat, 15 Jan 2022 21:52:46: 14000000 INFO @ Sat, 15 Jan 2022 21:52:48: 19000000 INFO @ Sat, 15 Jan 2022 21:52:52: 10000000 INFO @ Sat, 15 Jan 2022 21:52:54: 15000000 INFO @ Sat, 15 Jan 2022 21:52:56: 20000000 INFO @ Sat, 15 Jan 2022 21:53:00: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:53:01: 16000000 INFO @ Sat, 15 Jan 2022 21:53:03: 21000000 INFO @ Sat, 15 Jan 2022 21:53:08: 12000000 INFO @ Sat, 15 Jan 2022 21:53:09: 17000000 INFO @ Sat, 15 Jan 2022 21:53:11: 22000000 INFO @ Sat, 15 Jan 2022 21:53:16: 13000000 INFO @ Sat, 15 Jan 2022 21:53:17: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:53:19: 23000000 INFO @ Sat, 15 Jan 2022 21:53:24: 14000000 INFO @ Sat, 15 Jan 2022 21:53:25: 19000000 INFO @ Sat, 15 Jan 2022 21:53:27: 24000000 INFO @ Sat, 15 Jan 2022 21:53:31: 15000000 INFO @ Sat, 15 Jan 2022 21:53:32: 20000000 INFO @ Sat, 15 Jan 2022 21:53:34: 25000000 INFO @ Sat, 15 Jan 2022 21:53:38: 16000000 INFO @ Sat, 15 Jan 2022 21:53:39: 21000000 INFO @ Sat, 15 Jan 2022 21:53:41: 26000000 INFO @ Sat, 15 Jan 2022 21:53:44: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:53:44: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:53:44: #1 total tags in treatment: 12896307 INFO @ Sat, 15 Jan 2022 21:53:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:53:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:53:45: #1 tags after filtering in treatment: 6381612 INFO @ Sat, 15 Jan 2022 21:53:45: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 21:53:45: #1 finished! INFO @ Sat, 15 Jan 2022 21:53:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:53:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:53:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:53:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:53:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:53:45: 17000000 INFO @ Sat, 15 Jan 2022 21:53:47: 22000000 INFO @ Sat, 15 Jan 2022 21:53:52: 18000000 INFO @ Sat, 15 Jan 2022 21:53:54: 23000000 INFO @ Sat, 15 Jan 2022 21:53:59: 19000000 INFO @ Sat, 15 Jan 2022 21:54:01: 24000000 INFO @ Sat, 15 Jan 2022 21:54:06: 20000000 INFO @ Sat, 15 Jan 2022 21:54:08: 25000000 INFO @ Sat, 15 Jan 2022 21:54:13: 21000000 INFO @ Sat, 15 Jan 2022 21:54:15: 26000000 INFO @ Sat, 15 Jan 2022 21:54:18: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:54:18: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:54:18: #1 total tags in treatment: 12896307 INFO @ Sat, 15 Jan 2022 21:54:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:54:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:54:18: #1 tags after filtering in treatment: 6381612 INFO @ Sat, 15 Jan 2022 21:54:18: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 21:54:18: #1 finished! INFO @ Sat, 15 Jan 2022 21:54:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:54:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:54:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:54:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:54:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:54:20: 22000000 INFO @ Sat, 15 Jan 2022 21:54:26: 23000000 INFO @ Sat, 15 Jan 2022 21:54:32: 24000000 INFO @ Sat, 15 Jan 2022 21:54:39: 25000000 INFO @ Sat, 15 Jan 2022 21:54:45: 26000000 INFO @ Sat, 15 Jan 2022 21:54:47: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:54:47: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:54:47: #1 total tags in treatment: 12896307 INFO @ Sat, 15 Jan 2022 21:54:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:54:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:54:48: #1 tags after filtering in treatment: 6381612 INFO @ Sat, 15 Jan 2022 21:54:48: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 15 Jan 2022 21:54:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:54:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:54:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:54:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:54:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:54:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754393/SRX10754393.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling