Job ID = 14521470 SRX = SRX10754389 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7457599 spots for SRR14402186/SRR14402186.sra Written 7457599 spots for SRR14402186/SRR14402186.sra Read 7115377 spots for SRR14402187/SRR14402187.sra Written 7115377 spots for SRR14402187/SRR14402187.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:40 14572976 reads; of these: 14572976 (100.00%) were paired; of these: 635587 (4.36%) aligned concordantly 0 times 11245638 (77.17%) aligned concordantly exactly 1 time 2691751 (18.47%) aligned concordantly >1 times ---- 635587 pairs aligned concordantly 0 times; of these: 127708 (20.09%) aligned discordantly 1 time ---- 507879 pairs aligned 0 times concordantly or discordantly; of these: 1015758 mates make up the pairs; of these: 793679 (78.14%) aligned 0 times 113184 (11.14%) aligned exactly 1 time 108895 (10.72%) aligned >1 times 97.28% overall alignment rate Time searching: 00:08:40 Overall time: 00:08:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2991839 / 14033879 = 0.2132 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:22:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:22:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:22:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:22:50: 1000000 INFO @ Sat, 15 Jan 2022 21:22:55: 2000000 INFO @ Sat, 15 Jan 2022 21:23:00: 3000000 INFO @ Sat, 15 Jan 2022 21:23:05: 4000000 INFO @ Sat, 15 Jan 2022 21:23:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:23:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:23:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:23:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:23:16: 6000000 INFO @ Sat, 15 Jan 2022 21:23:19: 1000000 INFO @ Sat, 15 Jan 2022 21:23:22: 7000000 INFO @ Sat, 15 Jan 2022 21:23:24: 2000000 INFO @ Sat, 15 Jan 2022 21:23:27: 8000000 INFO @ Sat, 15 Jan 2022 21:23:29: 3000000 INFO @ Sat, 15 Jan 2022 21:23:33: 9000000 INFO @ Sat, 15 Jan 2022 21:23:33: 4000000 INFO @ Sat, 15 Jan 2022 21:23:38: 5000000 INFO @ Sat, 15 Jan 2022 21:23:38: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:23:43: 6000000 INFO @ Sat, 15 Jan 2022 21:23:44: 11000000 INFO @ Sat, 15 Jan 2022 21:23:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:23:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:23:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:23:47: 7000000 INFO @ Sat, 15 Jan 2022 21:23:49: 1000000 INFO @ Sat, 15 Jan 2022 21:23:50: 12000000 INFO @ Sat, 15 Jan 2022 21:23:52: 8000000 INFO @ Sat, 15 Jan 2022 21:23:54: 2000000 INFO @ Sat, 15 Jan 2022 21:23:55: 13000000 INFO @ Sat, 15 Jan 2022 21:23:57: 9000000 INFO @ Sat, 15 Jan 2022 21:23:59: 3000000 INFO @ Sat, 15 Jan 2022 21:24:01: 14000000 INFO @ Sat, 15 Jan 2022 21:24:02: 10000000 INFO @ Sat, 15 Jan 2022 21:24:04: 4000000 INFO @ Sat, 15 Jan 2022 21:24:06: 11000000 INFO @ Sat, 15 Jan 2022 21:24:07: 15000000 INFO @ Sat, 15 Jan 2022 21:24:08: 5000000 INFO @ Sat, 15 Jan 2022 21:24:11: 12000000 INFO @ Sat, 15 Jan 2022 21:24:12: 16000000 INFO @ Sat, 15 Jan 2022 21:24:13: 6000000 INFO @ Sat, 15 Jan 2022 21:24:16: 13000000 INFO @ Sat, 15 Jan 2022 21:24:18: 17000000 INFO @ Sat, 15 Jan 2022 21:24:18: 7000000 INFO @ Sat, 15 Jan 2022 21:24:21: 14000000 INFO @ Sat, 15 Jan 2022 21:24:23: 8000000 INFO @ Sat, 15 Jan 2022 21:24:23: 18000000 INFO @ Sat, 15 Jan 2022 21:24:26: 15000000 INFO @ Sat, 15 Jan 2022 21:24:27: 9000000 INFO @ Sat, 15 Jan 2022 21:24:29: 19000000 INFO @ Sat, 15 Jan 2022 21:24:31: 16000000 INFO @ Sat, 15 Jan 2022 21:24:32: 10000000 INFO @ Sat, 15 Jan 2022 21:24:35: 20000000 INFO @ Sat, 15 Jan 2022 21:24:35: 17000000 INFO @ Sat, 15 Jan 2022 21:24:37: 11000000 INFO @ Sat, 15 Jan 2022 21:24:40: 18000000 INFO @ Sat, 15 Jan 2022 21:24:40: 21000000 INFO @ Sat, 15 Jan 2022 21:24:42: 12000000 INFO @ Sat, 15 Jan 2022 21:24:45: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:24:46: 22000000 INFO @ Sat, 15 Jan 2022 21:24:46: 13000000 INFO @ Sat, 15 Jan 2022 21:24:48: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:24:48: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:24:48: #1 total tags in treatment: 10969121 INFO @ Sat, 15 Jan 2022 21:24:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:24:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:24:48: #1 tags after filtering in treatment: 6169617 INFO @ Sat, 15 Jan 2022 21:24:48: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 21:24:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:24:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:24:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:24:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:24:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:24:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:24:49: 20000000 INFO @ Sat, 15 Jan 2022 21:24:51: 14000000 INFO @ Sat, 15 Jan 2022 21:24:54: 21000000 INFO @ Sat, 15 Jan 2022 21:24:55: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:24:59: 22000000 INFO @ Sat, 15 Jan 2022 21:25:00: 16000000 INFO @ Sat, 15 Jan 2022 21:25:00: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:25:00: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:25:00: #1 total tags in treatment: 10969121 INFO @ Sat, 15 Jan 2022 21:25:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:25:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:25:01: #1 tags after filtering in treatment: 6169617 INFO @ Sat, 15 Jan 2022 21:25:01: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 21:25:01: #1 finished! INFO @ Sat, 15 Jan 2022 21:25:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:25:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:25:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:25:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:25:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:25:05: 17000000 INFO @ Sat, 15 Jan 2022 21:25:09: 18000000 INFO @ Sat, 15 Jan 2022 21:25:14: 19000000 INFO @ Sat, 15 Jan 2022 21:25:18: 20000000 INFO @ Sat, 15 Jan 2022 21:25:23: 21000000 INFO @ Sat, 15 Jan 2022 21:25:27: 22000000 INFO @ Sat, 15 Jan 2022 21:25:29: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:25:29: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:25:29: #1 total tags in treatment: 10969121 INFO @ Sat, 15 Jan 2022 21:25:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:25:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:25:29: #1 tags after filtering in treatment: 6169617 INFO @ Sat, 15 Jan 2022 21:25:29: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 21:25:29: #1 finished! INFO @ Sat, 15 Jan 2022 21:25:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:25:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:25:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:25:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:25:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754389/SRX10754389.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling