Job ID = 14521469 SRX = SRX10754388 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8229840 spots for SRR14402184/SRR14402184.sra Written 8229840 spots for SRR14402184/SRR14402184.sra Read 7825456 spots for SRR14402185/SRR14402185.sra Written 7825456 spots for SRR14402185/SRR14402185.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:09 16055296 reads; of these: 16055296 (100.00%) were paired; of these: 660216 (4.11%) aligned concordantly 0 times 12436892 (77.46%) aligned concordantly exactly 1 time 2958188 (18.42%) aligned concordantly >1 times ---- 660216 pairs aligned concordantly 0 times; of these: 164979 (24.99%) aligned discordantly 1 time ---- 495237 pairs aligned 0 times concordantly or discordantly; of these: 990474 mates make up the pairs; of these: 719157 (72.61%) aligned 0 times 125268 (12.65%) aligned exactly 1 time 146049 (14.75%) aligned >1 times 97.76% overall alignment rate Time searching: 00:14:09 Overall time: 00:14:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3010616 / 15502250 = 0.1942 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:28:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:28:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:28:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:28:20: 1000000 INFO @ Sat, 15 Jan 2022 21:28:29: 2000000 INFO @ Sat, 15 Jan 2022 21:28:37: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:28:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:28:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:28:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:28:45: 4000000 INFO @ Sat, 15 Jan 2022 21:28:49: 1000000 INFO @ Sat, 15 Jan 2022 21:28:54: 5000000 INFO @ Sat, 15 Jan 2022 21:28:57: 2000000 INFO @ Sat, 15 Jan 2022 21:29:02: 6000000 INFO @ Sat, 15 Jan 2022 21:29:05: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:11: 7000000 INFO @ Sat, 15 Jan 2022 21:29:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:13: 4000000 INFO @ Sat, 15 Jan 2022 21:29:19: 1000000 INFO @ Sat, 15 Jan 2022 21:29:20: 8000000 INFO @ Sat, 15 Jan 2022 21:29:21: 5000000 INFO @ Sat, 15 Jan 2022 21:29:26: 2000000 INFO @ Sat, 15 Jan 2022 21:29:28: 9000000 INFO @ Sat, 15 Jan 2022 21:29:29: 6000000 INFO @ Sat, 15 Jan 2022 21:29:33: 3000000 INFO @ Sat, 15 Jan 2022 21:29:36: 7000000 INFO @ Sat, 15 Jan 2022 21:29:37: 10000000 INFO @ Sat, 15 Jan 2022 21:29:39: 4000000 INFO @ Sat, 15 Jan 2022 21:29:44: 8000000 INFO @ Sat, 15 Jan 2022 21:29:46: 11000000 INFO @ Sat, 15 Jan 2022 21:29:47: 5000000 INFO @ Sat, 15 Jan 2022 21:29:51: 9000000 INFO @ Sat, 15 Jan 2022 21:29:54: 6000000 INFO @ Sat, 15 Jan 2022 21:29:54: 12000000 INFO @ Sat, 15 Jan 2022 21:29:59: 10000000 INFO @ Sat, 15 Jan 2022 21:30:02: 7000000 INFO @ Sat, 15 Jan 2022 21:30:03: 13000000 INFO @ Sat, 15 Jan 2022 21:30:06: 11000000 INFO @ Sat, 15 Jan 2022 21:30:08: 8000000 INFO @ Sat, 15 Jan 2022 21:30:11: 14000000 INFO @ Sat, 15 Jan 2022 21:30:14: 12000000 INFO @ Sat, 15 Jan 2022 21:30:15: 9000000 INFO @ Sat, 15 Jan 2022 21:30:20: 15000000 INFO @ Sat, 15 Jan 2022 21:30:22: 13000000 INFO @ Sat, 15 Jan 2022 21:30:23: 10000000 INFO @ Sat, 15 Jan 2022 21:30:29: 16000000 INFO @ Sat, 15 Jan 2022 21:30:30: 14000000 INFO @ Sat, 15 Jan 2022 21:30:31: 11000000 INFO @ Sat, 15 Jan 2022 21:30:37: 15000000 INFO @ Sat, 15 Jan 2022 21:30:38: 17000000 INFO @ Sat, 15 Jan 2022 21:30:41: 12000000 INFO @ Sat, 15 Jan 2022 21:30:45: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:30:48: 18000000 INFO @ Sat, 15 Jan 2022 21:30:51: 13000000 INFO @ Sat, 15 Jan 2022 21:30:53: 17000000 INFO @ Sat, 15 Jan 2022 21:30:58: 19000000 INFO @ Sat, 15 Jan 2022 21:31:00: 14000000 INFO @ Sat, 15 Jan 2022 21:31:01: 18000000 INFO @ Sat, 15 Jan 2022 21:31:07: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:31:09: 19000000 INFO @ Sat, 15 Jan 2022 21:31:10: 15000000 INFO @ Sat, 15 Jan 2022 21:31:17: 21000000 INFO @ Sat, 15 Jan 2022 21:31:17: 20000000 INFO @ Sat, 15 Jan 2022 21:31:19: 16000000 INFO @ Sat, 15 Jan 2022 21:31:25: 21000000 INFO @ Sat, 15 Jan 2022 21:31:26: 22000000 INFO @ Sat, 15 Jan 2022 21:31:28: 17000000 INFO @ Sat, 15 Jan 2022 21:31:33: 22000000 INFO @ Sat, 15 Jan 2022 21:31:36: 23000000 INFO @ Sat, 15 Jan 2022 21:31:38: 18000000 INFO @ Sat, 15 Jan 2022 21:31:41: 23000000 INFO @ Sat, 15 Jan 2022 21:31:45: 24000000 INFO @ Sat, 15 Jan 2022 21:31:46: 19000000 INFO @ Sat, 15 Jan 2022 21:31:49: 24000000 INFO @ Sat, 15 Jan 2022 21:31:54: 25000000 INFO @ Sat, 15 Jan 2022 21:31:56: 20000000 INFO @ Sat, 15 Jan 2022 21:31:57: 25000000 INFO @ Sat, 15 Jan 2022 21:31:58: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:31:58: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:31:58: #1 total tags in treatment: 12410084 INFO @ Sat, 15 Jan 2022 21:31:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:31:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:31:59: #1 tags after filtering in treatment: 8184053 INFO @ Sat, 15 Jan 2022 21:31:59: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 21:31:59: #1 finished! INFO @ Sat, 15 Jan 2022 21:31:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:31:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:31:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:31:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:31:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:32:00: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:32:00: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:32:00: #1 total tags in treatment: 12410084 INFO @ Sat, 15 Jan 2022 21:32:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:32:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:32:00: #1 tags after filtering in treatment: 8184053 INFO @ Sat, 15 Jan 2022 21:32:00: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 21:32:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:32:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:32:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:32:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:32:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:32:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:32:05: 21000000 INFO @ Sat, 15 Jan 2022 21:32:13: 22000000 INFO @ Sat, 15 Jan 2022 21:32:22: 23000000 INFO @ Sat, 15 Jan 2022 21:32:30: 24000000 INFO @ Sat, 15 Jan 2022 21:32:39: 25000000 INFO @ Sat, 15 Jan 2022 21:32:42: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:32:42: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:32:42: #1 total tags in treatment: 12410084 INFO @ Sat, 15 Jan 2022 21:32:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:32:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:32:42: #1 tags after filtering in treatment: 8184053 INFO @ Sat, 15 Jan 2022 21:32:42: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 21:32:42: #1 finished! INFO @ Sat, 15 Jan 2022 21:32:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:32:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:32:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:32:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:32:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754388/SRX10754388.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling