Job ID = 14521468 SRX = SRX10754387 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7554504 spots for SRR14402182/SRR14402182.sra Written 7554504 spots for SRR14402182/SRR14402182.sra Read 7165781 spots for SRR14402183/SRR14402183.sra Written 7165781 spots for SRR14402183/SRR14402183.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:49 14720285 reads; of these: 14720285 (100.00%) were paired; of these: 839335 (5.70%) aligned concordantly 0 times 11281084 (76.64%) aligned concordantly exactly 1 time 2599866 (17.66%) aligned concordantly >1 times ---- 839335 pairs aligned concordantly 0 times; of these: 160468 (19.12%) aligned discordantly 1 time ---- 678867 pairs aligned 0 times concordantly or discordantly; of these: 1357734 mates make up the pairs; of these: 1107104 (81.54%) aligned 0 times 123419 (9.09%) aligned exactly 1 time 127211 (9.37%) aligned >1 times 96.24% overall alignment rate Time searching: 00:11:49 Overall time: 00:11:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2465938 / 13982068 = 0.1764 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:24:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:24:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:24:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:24:21: 1000000 INFO @ Sat, 15 Jan 2022 21:24:26: 2000000 INFO @ Sat, 15 Jan 2022 21:24:32: 3000000 INFO @ Sat, 15 Jan 2022 21:24:37: 4000000 INFO @ Sat, 15 Jan 2022 21:24:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:24:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:24:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:24:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:24:47: 6000000 INFO @ Sat, 15 Jan 2022 21:24:51: 1000000 INFO @ Sat, 15 Jan 2022 21:24:52: 7000000 INFO @ Sat, 15 Jan 2022 21:24:57: 2000000 INFO @ Sat, 15 Jan 2022 21:24:57: 8000000 INFO @ Sat, 15 Jan 2022 21:25:02: 3000000 INFO @ Sat, 15 Jan 2022 21:25:02: 9000000 INFO @ Sat, 15 Jan 2022 21:25:08: 10000000 INFO @ Sat, 15 Jan 2022 21:25:08: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:25:13: 11000000 INFO @ Sat, 15 Jan 2022 21:25:13: 5000000 INFO @ Sat, 15 Jan 2022 21:25:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:25:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:25:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:25:18: 12000000 INFO @ Sat, 15 Jan 2022 21:25:20: 6000000 INFO @ Sat, 15 Jan 2022 21:25:21: 1000000 INFO @ Sat, 15 Jan 2022 21:25:24: 13000000 INFO @ Sat, 15 Jan 2022 21:25:26: 7000000 INFO @ Sat, 15 Jan 2022 21:25:26: 2000000 INFO @ Sat, 15 Jan 2022 21:25:30: 14000000 INFO @ Sat, 15 Jan 2022 21:25:31: 8000000 INFO @ Sat, 15 Jan 2022 21:25:32: 3000000 INFO @ Sat, 15 Jan 2022 21:25:35: 15000000 INFO @ Sat, 15 Jan 2022 21:25:37: 9000000 INFO @ Sat, 15 Jan 2022 21:25:37: 4000000 INFO @ Sat, 15 Jan 2022 21:25:41: 16000000 INFO @ Sat, 15 Jan 2022 21:25:43: 10000000 INFO @ Sat, 15 Jan 2022 21:25:43: 5000000 INFO @ Sat, 15 Jan 2022 21:25:47: 17000000 INFO @ Sat, 15 Jan 2022 21:25:49: 6000000 INFO @ Sat, 15 Jan 2022 21:25:49: 11000000 INFO @ Sat, 15 Jan 2022 21:25:53: 18000000 INFO @ Sat, 15 Jan 2022 21:25:54: 7000000 INFO @ Sat, 15 Jan 2022 21:25:54: 12000000 INFO @ Sat, 15 Jan 2022 21:25:58: 19000000 INFO @ Sat, 15 Jan 2022 21:26:00: 8000000 INFO @ Sat, 15 Jan 2022 21:26:00: 13000000 INFO @ Sat, 15 Jan 2022 21:26:05: 20000000 INFO @ Sat, 15 Jan 2022 21:26:06: 14000000 INFO @ Sat, 15 Jan 2022 21:26:06: 9000000 INFO @ Sat, 15 Jan 2022 21:26:11: 21000000 INFO @ Sat, 15 Jan 2022 21:26:11: 15000000 INFO @ Sat, 15 Jan 2022 21:26:13: 10000000 INFO @ Sat, 15 Jan 2022 21:26:17: 16000000 INFO @ Sat, 15 Jan 2022 21:26:17: 22000000 INFO @ Sat, 15 Jan 2022 21:26:19: 11000000 INFO @ Sat, 15 Jan 2022 21:26:23: 17000000 INFO @ Sat, 15 Jan 2022 21:26:23: 23000000 INFO @ Sat, 15 Jan 2022 21:26:25: 12000000 INFO @ Sat, 15 Jan 2022 21:26:26: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:26:26: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:26:26: #1 total tags in treatment: 11437792 INFO @ Sat, 15 Jan 2022 21:26:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:26:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:26:26: #1 tags after filtering in treatment: 7646042 INFO @ Sat, 15 Jan 2022 21:26:26: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 21:26:26: #1 finished! INFO @ Sat, 15 Jan 2022 21:26:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:26:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:26:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:26:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:26:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:26:30: 18000000 INFO @ Sat, 15 Jan 2022 21:26:31: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:26:36: 19000000 INFO @ Sat, 15 Jan 2022 21:26:37: 14000000 INFO @ Sat, 15 Jan 2022 21:26:42: 15000000 INFO @ Sat, 15 Jan 2022 21:26:43: 20000000 INFO @ Sat, 15 Jan 2022 21:26:47: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:26:50: 21000000 INFO @ Sat, 15 Jan 2022 21:26:52: 17000000 INFO @ Sat, 15 Jan 2022 21:26:57: 22000000 INFO @ Sat, 15 Jan 2022 21:26:58: 18000000 INFO @ Sat, 15 Jan 2022 21:27:03: 19000000 INFO @ Sat, 15 Jan 2022 21:27:04: 23000000 INFO @ Sat, 15 Jan 2022 21:27:07: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:27:07: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:27:07: #1 total tags in treatment: 11437792 INFO @ Sat, 15 Jan 2022 21:27:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:27:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:27:08: #1 tags after filtering in treatment: 7646042 INFO @ Sat, 15 Jan 2022 21:27:08: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 21:27:08: #1 finished! INFO @ Sat, 15 Jan 2022 21:27:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:27:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:27:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:27:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:27:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:27:09: 20000000 INFO @ Sat, 15 Jan 2022 21:27:14: 21000000 INFO @ Sat, 15 Jan 2022 21:27:20: 22000000 INFO @ Sat, 15 Jan 2022 21:27:26: 23000000 INFO @ Sat, 15 Jan 2022 21:27:28: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:27:28: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:27:28: #1 total tags in treatment: 11437792 INFO @ Sat, 15 Jan 2022 21:27:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:27:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:27:28: #1 tags after filtering in treatment: 7646042 INFO @ Sat, 15 Jan 2022 21:27:28: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 21:27:28: #1 finished! INFO @ Sat, 15 Jan 2022 21:27:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:27:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:27:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:27:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:27:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754387/SRX10754387.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling