Job ID = 14521467 SRX = SRX10754386 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7619365 spots for SRR14402180/SRR14402180.sra Written 7619365 spots for SRR14402180/SRR14402180.sra Read 7238800 spots for SRR14402181/SRR14402181.sra Written 7238800 spots for SRR14402181/SRR14402181.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:59 14858165 reads; of these: 14858165 (100.00%) were paired; of these: 628582 (4.23%) aligned concordantly 0 times 11463168 (77.15%) aligned concordantly exactly 1 time 2766415 (18.62%) aligned concordantly >1 times ---- 628582 pairs aligned concordantly 0 times; of these: 129872 (20.66%) aligned discordantly 1 time ---- 498710 pairs aligned 0 times concordantly or discordantly; of these: 997420 mates make up the pairs; of these: 774168 (77.62%) aligned 0 times 112235 (11.25%) aligned exactly 1 time 111017 (11.13%) aligned >1 times 97.39% overall alignment rate Time searching: 00:07:59 Overall time: 00:07:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3007058 / 14328232 = 0.2099 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:20:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:20:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:20:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:20:47: 1000000 INFO @ Sat, 15 Jan 2022 21:20:52: 2000000 INFO @ Sat, 15 Jan 2022 21:20:58: 3000000 INFO @ Sat, 15 Jan 2022 21:21:04: 4000000 INFO @ Sat, 15 Jan 2022 21:21:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:21:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:21:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:21:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:21:15: 6000000 INFO @ Sat, 15 Jan 2022 21:21:16: 1000000 INFO @ Sat, 15 Jan 2022 21:21:20: 7000000 INFO @ Sat, 15 Jan 2022 21:21:22: 2000000 INFO @ Sat, 15 Jan 2022 21:21:26: 8000000 INFO @ Sat, 15 Jan 2022 21:21:28: 3000000 INFO @ Sat, 15 Jan 2022 21:21:32: 9000000 INFO @ Sat, 15 Jan 2022 21:21:34: 4000000 INFO @ Sat, 15 Jan 2022 21:21:38: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:21:39: 5000000 INFO @ Sat, 15 Jan 2022 21:21:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:21:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:21:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:21:44: 11000000 INFO @ Sat, 15 Jan 2022 21:21:46: 6000000 INFO @ Sat, 15 Jan 2022 21:21:47: 1000000 INFO @ Sat, 15 Jan 2022 21:21:50: 12000000 INFO @ Sat, 15 Jan 2022 21:21:52: 7000000 INFO @ Sat, 15 Jan 2022 21:21:53: 2000000 INFO @ Sat, 15 Jan 2022 21:21:56: 13000000 INFO @ Sat, 15 Jan 2022 21:21:58: 8000000 INFO @ Sat, 15 Jan 2022 21:21:59: 3000000 INFO @ Sat, 15 Jan 2022 21:22:02: 14000000 INFO @ Sat, 15 Jan 2022 21:22:04: 9000000 INFO @ Sat, 15 Jan 2022 21:22:05: 4000000 INFO @ Sat, 15 Jan 2022 21:22:08: 15000000 INFO @ Sat, 15 Jan 2022 21:22:10: 10000000 INFO @ Sat, 15 Jan 2022 21:22:11: 5000000 INFO @ Sat, 15 Jan 2022 21:22:14: 16000000 INFO @ Sat, 15 Jan 2022 21:22:16: 11000000 INFO @ Sat, 15 Jan 2022 21:22:17: 6000000 INFO @ Sat, 15 Jan 2022 21:22:20: 17000000 INFO @ Sat, 15 Jan 2022 21:22:22: 12000000 INFO @ Sat, 15 Jan 2022 21:22:23: 7000000 INFO @ Sat, 15 Jan 2022 21:22:27: 18000000 INFO @ Sat, 15 Jan 2022 21:22:29: 13000000 INFO @ Sat, 15 Jan 2022 21:22:29: 8000000 INFO @ Sat, 15 Jan 2022 21:22:33: 19000000 INFO @ Sat, 15 Jan 2022 21:22:35: 14000000 INFO @ Sat, 15 Jan 2022 21:22:35: 9000000 INFO @ Sat, 15 Jan 2022 21:22:39: 20000000 INFO @ Sat, 15 Jan 2022 21:22:41: 15000000 INFO @ Sat, 15 Jan 2022 21:22:41: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:22:45: 21000000 INFO @ Sat, 15 Jan 2022 21:22:47: 16000000 INFO @ Sat, 15 Jan 2022 21:22:47: 11000000 INFO @ Sat, 15 Jan 2022 21:22:51: 22000000 INFO @ Sat, 15 Jan 2022 21:22:53: 12000000 INFO @ Sat, 15 Jan 2022 21:22:53: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:22:57: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:22:57: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:22:57: #1 total tags in treatment: 11247253 INFO @ Sat, 15 Jan 2022 21:22:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:22:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:22:57: #1 tags after filtering in treatment: 6709289 INFO @ Sat, 15 Jan 2022 21:22:57: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 21:22:57: #1 finished! INFO @ Sat, 15 Jan 2022 21:22:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:22:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:22:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:22:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:22:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:22:59: 13000000 INFO @ Sat, 15 Jan 2022 21:22:59: 18000000 INFO @ Sat, 15 Jan 2022 21:23:05: 14000000 INFO @ Sat, 15 Jan 2022 21:23:05: 19000000 INFO @ Sat, 15 Jan 2022 21:23:11: 15000000 INFO @ Sat, 15 Jan 2022 21:23:11: 20000000 INFO @ Sat, 15 Jan 2022 21:23:17: 16000000 INFO @ Sat, 15 Jan 2022 21:23:17: 21000000 INFO @ Sat, 15 Jan 2022 21:23:23: 17000000 INFO @ Sat, 15 Jan 2022 21:23:23: 22000000 INFO @ Sat, 15 Jan 2022 21:23:29: 18000000 INFO @ Sat, 15 Jan 2022 21:23:29: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:23:29: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:23:29: #1 total tags in treatment: 11247253 INFO @ Sat, 15 Jan 2022 21:23:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:23:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:23:29: #1 tags after filtering in treatment: 6709289 INFO @ Sat, 15 Jan 2022 21:23:29: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 21:23:29: #1 finished! INFO @ Sat, 15 Jan 2022 21:23:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:23:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:23:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:23:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:23:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:23:35: 19000000 INFO @ Sat, 15 Jan 2022 21:23:41: 20000000 INFO @ Sat, 15 Jan 2022 21:23:46: 21000000 INFO @ Sat, 15 Jan 2022 21:23:52: 22000000 INFO @ Sat, 15 Jan 2022 21:23:57: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:23:57: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:23:57: #1 total tags in treatment: 11247253 INFO @ Sat, 15 Jan 2022 21:23:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:23:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:23:57: #1 tags after filtering in treatment: 6709289 INFO @ Sat, 15 Jan 2022 21:23:57: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 21:23:57: #1 finished! INFO @ Sat, 15 Jan 2022 21:23:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:23:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:23:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:23:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:23:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754386/SRX10754386.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling