Job ID = 14521466 SRX = SRX10754385 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9077305 spots for SRR14402178/SRR14402178.sra Written 9077305 spots for SRR14402178/SRR14402178.sra Read 8651484 spots for SRR14402179/SRR14402179.sra Written 8651484 spots for SRR14402179/SRR14402179.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:56 17728789 reads; of these: 17728789 (100.00%) were paired; of these: 816084 (4.60%) aligned concordantly 0 times 13533709 (76.34%) aligned concordantly exactly 1 time 3378996 (19.06%) aligned concordantly >1 times ---- 816084 pairs aligned concordantly 0 times; of these: 140394 (17.20%) aligned discordantly 1 time ---- 675690 pairs aligned 0 times concordantly or discordantly; of these: 1351380 mates make up the pairs; of these: 1088764 (80.57%) aligned 0 times 144754 (10.71%) aligned exactly 1 time 117862 (8.72%) aligned >1 times 96.93% overall alignment rate Time searching: 00:15:56 Overall time: 00:15:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3730197 / 17019906 = 0.2192 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:30:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:30:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:30:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:31:07: 1000000 INFO @ Sat, 15 Jan 2022 21:31:17: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:31:27: 3000000 INFO @ Sat, 15 Jan 2022 21:31:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:31:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:31:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:31:37: 1000000 INFO @ Sat, 15 Jan 2022 21:31:37: 4000000 INFO @ Sat, 15 Jan 2022 21:31:47: 5000000 INFO @ Sat, 15 Jan 2022 21:31:47: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:31:56: 6000000 INFO @ Sat, 15 Jan 2022 21:31:57: 3000000 INFO @ Sat, 15 Jan 2022 21:31:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:31:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:31:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:32:05: 7000000 INFO @ Sat, 15 Jan 2022 21:32:06: 4000000 INFO @ Sat, 15 Jan 2022 21:32:09: 1000000 INFO @ Sat, 15 Jan 2022 21:32:14: 8000000 INFO @ Sat, 15 Jan 2022 21:32:16: 5000000 INFO @ Sat, 15 Jan 2022 21:32:21: 2000000 INFO @ Sat, 15 Jan 2022 21:32:24: 9000000 INFO @ Sat, 15 Jan 2022 21:32:25: 6000000 INFO @ Sat, 15 Jan 2022 21:32:33: 3000000 INFO @ Sat, 15 Jan 2022 21:32:33: 10000000 INFO @ Sat, 15 Jan 2022 21:32:33: 7000000 INFO @ Sat, 15 Jan 2022 21:32:42: 8000000 INFO @ Sat, 15 Jan 2022 21:32:43: 11000000 INFO @ Sat, 15 Jan 2022 21:32:45: 4000000 INFO @ Sat, 15 Jan 2022 21:32:50: 9000000 INFO @ Sat, 15 Jan 2022 21:32:52: 12000000 INFO @ Sat, 15 Jan 2022 21:32:57: 5000000 INFO @ Sat, 15 Jan 2022 21:32:59: 10000000 INFO @ Sat, 15 Jan 2022 21:33:01: 13000000 INFO @ Sat, 15 Jan 2022 21:33:08: 6000000 INFO @ Sat, 15 Jan 2022 21:33:09: 11000000 INFO @ Sat, 15 Jan 2022 21:33:11: 14000000 INFO @ Sat, 15 Jan 2022 21:33:19: 12000000 INFO @ Sat, 15 Jan 2022 21:33:20: 7000000 INFO @ Sat, 15 Jan 2022 21:33:21: 15000000 INFO @ Sat, 15 Jan 2022 21:33:28: 13000000 INFO @ Sat, 15 Jan 2022 21:33:29: 16000000 INFO @ Sat, 15 Jan 2022 21:33:32: 8000000 INFO @ Sat, 15 Jan 2022 21:33:37: 14000000 INFO @ Sat, 15 Jan 2022 21:33:38: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:33:44: 9000000 INFO @ Sat, 15 Jan 2022 21:33:46: 18000000 INFO @ Sat, 15 Jan 2022 21:33:48: 15000000 INFO @ Sat, 15 Jan 2022 21:33:55: 19000000 INFO @ Sat, 15 Jan 2022 21:33:56: 10000000 INFO @ Sat, 15 Jan 2022 21:33:57: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:34:05: 20000000 INFO @ Sat, 15 Jan 2022 21:34:08: 17000000 INFO @ Sat, 15 Jan 2022 21:34:09: 11000000 INFO @ Sat, 15 Jan 2022 21:34:14: 21000000 INFO @ Sat, 15 Jan 2022 21:34:21: 18000000 INFO @ Sat, 15 Jan 2022 21:34:22: 12000000 INFO @ Sat, 15 Jan 2022 21:34:23: 22000000 INFO @ Sat, 15 Jan 2022 21:34:32: 19000000 INFO @ Sat, 15 Jan 2022 21:34:34: 23000000 INFO @ Sat, 15 Jan 2022 21:34:35: 13000000 INFO @ Sat, 15 Jan 2022 21:34:42: 20000000 INFO @ Sat, 15 Jan 2022 21:34:43: 24000000 INFO @ Sat, 15 Jan 2022 21:34:48: 14000000 INFO @ Sat, 15 Jan 2022 21:34:52: 25000000 INFO @ Sat, 15 Jan 2022 21:34:53: 21000000 INFO @ Sat, 15 Jan 2022 21:35:00: 15000000 INFO @ Sat, 15 Jan 2022 21:35:02: 26000000 INFO @ Sat, 15 Jan 2022 21:35:04: 22000000 INFO @ Sat, 15 Jan 2022 21:35:11: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:35:11: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:35:11: #1 total tags in treatment: 13209381 INFO @ Sat, 15 Jan 2022 21:35:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:11: #1 tags after filtering in treatment: 7451019 INFO @ Sat, 15 Jan 2022 21:35:11: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 21:35:11: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:35:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:35:13: 16000000 INFO @ Sat, 15 Jan 2022 21:35:16: 23000000 INFO @ Sat, 15 Jan 2022 21:35:27: 24000000 INFO @ Sat, 15 Jan 2022 21:35:27: 17000000 INFO @ Sat, 15 Jan 2022 21:35:38: 25000000 INFO @ Sat, 15 Jan 2022 21:35:41: 18000000 INFO @ Sat, 15 Jan 2022 21:35:48: 26000000 INFO @ Sat, 15 Jan 2022 21:35:54: 19000000 INFO @ Sat, 15 Jan 2022 21:35:57: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:35:57: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:35:57: #1 total tags in treatment: 13209381 INFO @ Sat, 15 Jan 2022 21:35:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:58: #1 tags after filtering in treatment: 7451019 INFO @ Sat, 15 Jan 2022 21:35:58: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 21:35:58: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:35:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:36:08: 20000000 INFO @ Sat, 15 Jan 2022 21:36:22: 21000000 INFO @ Sat, 15 Jan 2022 21:36:35: 22000000 INFO @ Sat, 15 Jan 2022 21:36:47: 23000000 INFO @ Sat, 15 Jan 2022 21:36:58: 24000000 INFO @ Sat, 15 Jan 2022 21:37:10: 25000000 INFO @ Sat, 15 Jan 2022 21:37:22: 26000000 INFO @ Sat, 15 Jan 2022 21:37:32: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:37:32: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:37:32: #1 total tags in treatment: 13209381 INFO @ Sat, 15 Jan 2022 21:37:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:37:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:37:33: #1 tags after filtering in treatment: 7451019 INFO @ Sat, 15 Jan 2022 21:37:33: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 21:37:33: #1 finished! INFO @ Sat, 15 Jan 2022 21:37:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:37:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:37:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:37:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:37:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10754385/SRX10754385.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling