Job ID = 14520154 SRX = SRX10701694 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8897934 spots for SRR14347844/SRR14347844.sra Written 8897934 spots for SRR14347844/SRR14347844.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:44 8897934 reads; of these: 8897934 (100.00%) were paired; of these: 1361362 (15.30%) aligned concordantly 0 times 6069061 (68.21%) aligned concordantly exactly 1 time 1467511 (16.49%) aligned concordantly >1 times ---- 1361362 pairs aligned concordantly 0 times; of these: 59197 (4.35%) aligned discordantly 1 time ---- 1302165 pairs aligned 0 times concordantly or discordantly; of these: 2604330 mates make up the pairs; of these: 2526163 (97.00%) aligned 0 times 37016 (1.42%) aligned exactly 1 time 41151 (1.58%) aligned >1 times 85.80% overall alignment rate Time searching: 00:04:44 Overall time: 00:04:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2189534 / 7595181 = 0.2883 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:35:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:35:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:35:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:35:26: 1000000 INFO @ Sat, 15 Jan 2022 18:35:32: 2000000 INFO @ Sat, 15 Jan 2022 18:35:38: 3000000 INFO @ Sat, 15 Jan 2022 18:35:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:35:49: 5000000 INFO @ Sat, 15 Jan 2022 18:35:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:35:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:35:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:35:55: 6000000 INFO @ Sat, 15 Jan 2022 18:35:57: 1000000 INFO @ Sat, 15 Jan 2022 18:36:02: 7000000 INFO @ Sat, 15 Jan 2022 18:36:03: 2000000 INFO @ Sat, 15 Jan 2022 18:36:08: 8000000 INFO @ Sat, 15 Jan 2022 18:36:10: 3000000 INFO @ Sat, 15 Jan 2022 18:36:14: 9000000 INFO @ Sat, 15 Jan 2022 18:36:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:36:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:36:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:36:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:36:21: 10000000 INFO @ Sat, 15 Jan 2022 18:36:22: 5000000 INFO @ Sat, 15 Jan 2022 18:36:27: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:36:27: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:36:27: #1 total tags in treatment: 5353764 INFO @ Sat, 15 Jan 2022 18:36:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:36:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:36:27: #1 tags after filtering in treatment: 3498441 INFO @ Sat, 15 Jan 2022 18:36:27: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:36:27: #1 finished! INFO @ Sat, 15 Jan 2022 18:36:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:36:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:36:27: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 18:36:27: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:36:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:36:27: 1000000 INFO @ Sat, 15 Jan 2022 18:36:29: 6000000 INFO @ Sat, 15 Jan 2022 18:36:33: 2000000 INFO @ Sat, 15 Jan 2022 18:36:35: 7000000 INFO @ Sat, 15 Jan 2022 18:36:40: 3000000 INFO @ Sat, 15 Jan 2022 18:36:42: 8000000 INFO @ Sat, 15 Jan 2022 18:36:46: 4000000 INFO @ Sat, 15 Jan 2022 18:36:48: 9000000 INFO @ Sat, 15 Jan 2022 18:36:52: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:36:54: 10000000 INFO @ Sat, 15 Jan 2022 18:36:58: 6000000 INFO @ Sat, 15 Jan 2022 18:37:00: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:37:00: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:37:00: #1 total tags in treatment: 5353764 INFO @ Sat, 15 Jan 2022 18:37:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:37:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:37:00: #1 tags after filtering in treatment: 3498441 INFO @ Sat, 15 Jan 2022 18:37:00: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:37:00: #1 finished! INFO @ Sat, 15 Jan 2022 18:37:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:37:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:37:00: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 18:37:00: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:37:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:37:04: 7000000 INFO @ Sat, 15 Jan 2022 18:37:10: 8000000 INFO @ Sat, 15 Jan 2022 18:37:16: 9000000 INFO @ Sat, 15 Jan 2022 18:37:22: 10000000 INFO @ Sat, 15 Jan 2022 18:37:27: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:37:27: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:37:27: #1 total tags in treatment: 5353764 INFO @ Sat, 15 Jan 2022 18:37:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:37:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:37:27: #1 tags after filtering in treatment: 3498441 INFO @ Sat, 15 Jan 2022 18:37:27: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:37:27: #1 finished! INFO @ Sat, 15 Jan 2022 18:37:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:37:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:37:27: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 18:37:27: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:37:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701694/SRX10701694.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling