Job ID = 14520150 SRX = SRX10701690 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 50257927 spots for SRR14347840/SRR14347840.sra Written 50257927 spots for SRR14347840/SRR14347840.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:24 50257927 reads; of these: 50257927 (100.00%) were paired; of these: 5332484 (10.61%) aligned concordantly 0 times 38476130 (76.56%) aligned concordantly exactly 1 time 6449313 (12.83%) aligned concordantly >1 times ---- 5332484 pairs aligned concordantly 0 times; of these: 1621895 (30.42%) aligned discordantly 1 time ---- 3710589 pairs aligned 0 times concordantly or discordantly; of these: 7421178 mates make up the pairs; of these: 5050740 (68.06%) aligned 0 times 1513036 (20.39%) aligned exactly 1 time 857402 (11.55%) aligned >1 times 94.98% overall alignment rate Time searching: 00:29:24 Overall time: 00:29:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 26639960 / 46531390 = 0.5725 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:18:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:18:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:18:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:18:32: 1000000 INFO @ Sat, 15 Jan 2022 19:18:37: 2000000 INFO @ Sat, 15 Jan 2022 19:18:42: 3000000 INFO @ Sat, 15 Jan 2022 19:18:47: 4000000 INFO @ Sat, 15 Jan 2022 19:18:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:18:56: 6000000 INFO @ Sat, 15 Jan 2022 19:18:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:18:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:18:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:19:01: 7000000 INFO @ Sat, 15 Jan 2022 19:19:02: 1000000 INFO @ Sat, 15 Jan 2022 19:19:07: 8000000 INFO @ Sat, 15 Jan 2022 19:19:08: 2000000 INFO @ Sat, 15 Jan 2022 19:19:12: 9000000 INFO @ Sat, 15 Jan 2022 19:19:14: 3000000 INFO @ Sat, 15 Jan 2022 19:19:17: 10000000 INFO @ Sat, 15 Jan 2022 19:19:19: 4000000 INFO @ Sat, 15 Jan 2022 19:19:23: 11000000 INFO @ Sat, 15 Jan 2022 19:19:25: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:19:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:19:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:19:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:19:28: 12000000 INFO @ Sat, 15 Jan 2022 19:19:30: 6000000 INFO @ Sat, 15 Jan 2022 19:19:33: 1000000 INFO @ Sat, 15 Jan 2022 19:19:34: 13000000 INFO @ Sat, 15 Jan 2022 19:19:36: 7000000 INFO @ Sat, 15 Jan 2022 19:19:39: 2000000 INFO @ Sat, 15 Jan 2022 19:19:40: 14000000 INFO @ Sat, 15 Jan 2022 19:19:42: 8000000 INFO @ Sat, 15 Jan 2022 19:19:45: 3000000 INFO @ Sat, 15 Jan 2022 19:19:46: 15000000 INFO @ Sat, 15 Jan 2022 19:19:47: 9000000 INFO @ Sat, 15 Jan 2022 19:19:51: 4000000 INFO @ Sat, 15 Jan 2022 19:19:53: 16000000 INFO @ Sat, 15 Jan 2022 19:19:53: 10000000 INFO @ Sat, 15 Jan 2022 19:19:58: 5000000 INFO @ Sat, 15 Jan 2022 19:19:59: 11000000 INFO @ Sat, 15 Jan 2022 19:19:59: 17000000 INFO @ Sat, 15 Jan 2022 19:20:04: 6000000 INFO @ Sat, 15 Jan 2022 19:20:04: 12000000 INFO @ Sat, 15 Jan 2022 19:20:06: 18000000 INFO @ Sat, 15 Jan 2022 19:20:10: 13000000 INFO @ Sat, 15 Jan 2022 19:20:10: 7000000 INFO @ Sat, 15 Jan 2022 19:20:12: 19000000 INFO @ Sat, 15 Jan 2022 19:20:16: 8000000 INFO @ Sat, 15 Jan 2022 19:20:17: 14000000 INFO @ Sat, 15 Jan 2022 19:20:18: 20000000 INFO @ Sat, 15 Jan 2022 19:20:23: 9000000 INFO @ Sat, 15 Jan 2022 19:20:23: 15000000 INFO @ Sat, 15 Jan 2022 19:20:25: 21000000 INFO @ Sat, 15 Jan 2022 19:20:29: 10000000 INFO @ Sat, 15 Jan 2022 19:20:29: 16000000 INFO @ Sat, 15 Jan 2022 19:20:32: 22000000 INFO @ Sat, 15 Jan 2022 19:20:35: 11000000 INFO @ Sat, 15 Jan 2022 19:20:36: 17000000 INFO @ Sat, 15 Jan 2022 19:20:38: 23000000 INFO @ Sat, 15 Jan 2022 19:20:42: 12000000 INFO @ Sat, 15 Jan 2022 19:20:43: 18000000 INFO @ Sat, 15 Jan 2022 19:20:44: 24000000 INFO @ Sat, 15 Jan 2022 19:20:48: 13000000 INFO @ Sat, 15 Jan 2022 19:20:49: 19000000 INFO @ Sat, 15 Jan 2022 19:20:50: 25000000 INFO @ Sat, 15 Jan 2022 19:20:54: 14000000 INFO @ Sat, 15 Jan 2022 19:20:55: 20000000 INFO @ Sat, 15 Jan 2022 19:20:56: 26000000 INFO @ Sat, 15 Jan 2022 19:20:59: 15000000 INFO @ Sat, 15 Jan 2022 19:21:01: 27000000 INFO @ Sat, 15 Jan 2022 19:21:02: 21000000 INFO @ Sat, 15 Jan 2022 19:21:05: 16000000 INFO @ Sat, 15 Jan 2022 19:21:07: 28000000 INFO @ Sat, 15 Jan 2022 19:21:09: 22000000 INFO @ Sat, 15 Jan 2022 19:21:11: 17000000 INFO @ Sat, 15 Jan 2022 19:21:12: 29000000 INFO @ Sat, 15 Jan 2022 19:21:15: 23000000 INFO @ Sat, 15 Jan 2022 19:21:17: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:21:18: 30000000 INFO @ Sat, 15 Jan 2022 19:21:22: 24000000 INFO @ Sat, 15 Jan 2022 19:21:22: 19000000 INFO @ Sat, 15 Jan 2022 19:21:23: 31000000 INFO @ Sat, 15 Jan 2022 19:21:27: 25000000 INFO @ Sat, 15 Jan 2022 19:21:28: 20000000 INFO @ Sat, 15 Jan 2022 19:21:29: 32000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:21:33: 26000000 INFO @ Sat, 15 Jan 2022 19:21:34: 21000000 INFO @ Sat, 15 Jan 2022 19:21:35: 33000000 INFO @ Sat, 15 Jan 2022 19:21:40: 27000000 INFO @ Sat, 15 Jan 2022 19:21:40: 22000000 INFO @ Sat, 15 Jan 2022 19:21:41: 34000000 INFO @ Sat, 15 Jan 2022 19:21:46: 28000000 INFO @ Sat, 15 Jan 2022 19:21:47: 23000000 INFO @ Sat, 15 Jan 2022 19:21:47: 35000000 INFO @ Sat, 15 Jan 2022 19:21:52: 29000000 INFO @ Sat, 15 Jan 2022 19:21:53: 24000000 INFO @ Sat, 15 Jan 2022 19:21:53: 36000000 INFO @ Sat, 15 Jan 2022 19:21:57: 30000000 INFO @ Sat, 15 Jan 2022 19:21:59: 37000000 INFO @ Sat, 15 Jan 2022 19:22:00: 25000000 INFO @ Sat, 15 Jan 2022 19:22:03: 31000000 INFO @ Sat, 15 Jan 2022 19:22:05: 38000000 INFO @ Sat, 15 Jan 2022 19:22:07: 26000000 INFO @ Sat, 15 Jan 2022 19:22:08: 32000000 INFO @ Sat, 15 Jan 2022 19:22:11: 39000000 INFO @ Sat, 15 Jan 2022 19:22:13: 27000000 INFO @ Sat, 15 Jan 2022 19:22:14: 33000000 INFO @ Sat, 15 Jan 2022 19:22:16: 40000000 INFO @ Sat, 15 Jan 2022 19:22:19: 28000000 INFO @ Sat, 15 Jan 2022 19:22:19: 34000000 INFO @ Sat, 15 Jan 2022 19:22:22: 41000000 INFO @ Sat, 15 Jan 2022 19:22:25: 35000000 INFO @ Sat, 15 Jan 2022 19:22:25: 29000000 INFO @ Sat, 15 Jan 2022 19:22:27: 42000000 INFO @ Sat, 15 Jan 2022 19:22:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:22:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:22:29: #1 total tags in treatment: 19174992 INFO @ Sat, 15 Jan 2022 19:22:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:22:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:22:29: #1 tags after filtering in treatment: 7485923 INFO @ Sat, 15 Jan 2022 19:22:29: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 19:22:29: #1 finished! INFO @ Sat, 15 Jan 2022 19:22:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:22:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:22:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:22:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:22:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:22:31: 30000000 INFO @ Sat, 15 Jan 2022 19:22:31: 36000000 INFO @ Sat, 15 Jan 2022 19:22:37: 37000000 INFO @ Sat, 15 Jan 2022 19:22:37: 31000000 INFO @ Sat, 15 Jan 2022 19:22:43: 38000000 INFO @ Sat, 15 Jan 2022 19:22:44: 32000000 INFO @ Sat, 15 Jan 2022 19:22:48: 39000000 INFO @ Sat, 15 Jan 2022 19:22:50: 33000000 INFO @ Sat, 15 Jan 2022 19:22:54: 40000000 INFO @ Sat, 15 Jan 2022 19:22:56: 34000000 INFO @ Sat, 15 Jan 2022 19:22:59: 41000000 INFO @ Sat, 15 Jan 2022 19:23:02: 35000000 INFO @ Sat, 15 Jan 2022 19:23:04: 42000000 INFO @ Sat, 15 Jan 2022 19:23:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:23:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:23:06: #1 total tags in treatment: 19174992 INFO @ Sat, 15 Jan 2022 19:23:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:23:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:23:06: #1 tags after filtering in treatment: 7485923 INFO @ Sat, 15 Jan 2022 19:23:06: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 19:23:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:23:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:23:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:23:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:23:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:23:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:23:07: 36000000 INFO @ Sat, 15 Jan 2022 19:23:13: 37000000 INFO @ Sat, 15 Jan 2022 19:23:19: 38000000 INFO @ Sat, 15 Jan 2022 19:23:24: 39000000 INFO @ Sat, 15 Jan 2022 19:23:30: 40000000 INFO @ Sat, 15 Jan 2022 19:23:36: 41000000 INFO @ Sat, 15 Jan 2022 19:23:42: 42000000 INFO @ Sat, 15 Jan 2022 19:23:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:23:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:23:43: #1 total tags in treatment: 19174992 INFO @ Sat, 15 Jan 2022 19:23:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:23:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:23:43: #1 tags after filtering in treatment: 7485923 INFO @ Sat, 15 Jan 2022 19:23:43: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 19:23:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:23:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:23:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:23:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:23:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:23:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701690/SRX10701690.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling