Job ID = 14520145 SRX = SRX10701689 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 48195158 spots for SRR14347839/SRR14347839.sra Written 48195158 spots for SRR14347839/SRR14347839.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:26 48195158 reads; of these: 48195158 (100.00%) were paired; of these: 4784488 (9.93%) aligned concordantly 0 times 37065730 (76.91%) aligned concordantly exactly 1 time 6344940 (13.17%) aligned concordantly >1 times ---- 4784488 pairs aligned concordantly 0 times; of these: 1232767 (25.77%) aligned discordantly 1 time ---- 3551721 pairs aligned 0 times concordantly or discordantly; of these: 7103442 mates make up the pairs; of these: 5150418 (72.51%) aligned 0 times 1254096 (17.65%) aligned exactly 1 time 698928 (9.84%) aligned >1 times 94.66% overall alignment rate Time searching: 00:27:26 Overall time: 00:27:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 24033997 / 44627729 = 0.5385 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:13:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:13:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:13:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:14:05: 1000000 INFO @ Sat, 15 Jan 2022 19:14:11: 2000000 INFO @ Sat, 15 Jan 2022 19:14:18: 3000000 INFO @ Sat, 15 Jan 2022 19:14:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:14:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:14:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:14:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:14:31: 5000000 INFO @ Sat, 15 Jan 2022 19:14:35: 1000000 INFO @ Sat, 15 Jan 2022 19:14:38: 6000000 INFO @ Sat, 15 Jan 2022 19:14:41: 2000000 INFO @ Sat, 15 Jan 2022 19:14:46: 7000000 INFO @ Sat, 15 Jan 2022 19:14:48: 3000000 INFO @ Sat, 15 Jan 2022 19:14:53: 8000000 INFO @ Sat, 15 Jan 2022 19:14:54: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:14:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:14:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:14:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:00: 9000000 INFO @ Sat, 15 Jan 2022 19:15:00: 5000000 INFO @ Sat, 15 Jan 2022 19:15:05: 1000000 INFO @ Sat, 15 Jan 2022 19:15:07: 6000000 INFO @ Sat, 15 Jan 2022 19:15:08: 10000000 INFO @ Sat, 15 Jan 2022 19:15:12: 2000000 INFO @ Sat, 15 Jan 2022 19:15:14: 7000000 INFO @ Sat, 15 Jan 2022 19:15:15: 11000000 INFO @ Sat, 15 Jan 2022 19:15:19: 3000000 INFO @ Sat, 15 Jan 2022 19:15:21: 8000000 INFO @ Sat, 15 Jan 2022 19:15:23: 12000000 INFO @ Sat, 15 Jan 2022 19:15:26: 4000000 INFO @ Sat, 15 Jan 2022 19:15:28: 9000000 INFO @ Sat, 15 Jan 2022 19:15:30: 13000000 INFO @ Sat, 15 Jan 2022 19:15:32: 5000000 INFO @ Sat, 15 Jan 2022 19:15:35: 10000000 INFO @ Sat, 15 Jan 2022 19:15:37: 14000000 INFO @ Sat, 15 Jan 2022 19:15:39: 6000000 INFO @ Sat, 15 Jan 2022 19:15:41: 11000000 INFO @ Sat, 15 Jan 2022 19:15:44: 15000000 INFO @ Sat, 15 Jan 2022 19:15:46: 7000000 INFO @ Sat, 15 Jan 2022 19:15:48: 12000000 INFO @ Sat, 15 Jan 2022 19:15:51: 16000000 INFO @ Sat, 15 Jan 2022 19:15:53: 8000000 INFO @ Sat, 15 Jan 2022 19:15:55: 13000000 INFO @ Sat, 15 Jan 2022 19:15:59: 17000000 INFO @ Sat, 15 Jan 2022 19:16:00: 9000000 INFO @ Sat, 15 Jan 2022 19:16:02: 14000000 INFO @ Sat, 15 Jan 2022 19:16:06: 18000000 INFO @ Sat, 15 Jan 2022 19:16:07: 10000000 INFO @ Sat, 15 Jan 2022 19:16:09: 15000000 INFO @ Sat, 15 Jan 2022 19:16:13: 19000000 INFO @ Sat, 15 Jan 2022 19:16:14: 11000000 INFO @ Sat, 15 Jan 2022 19:16:16: 16000000 INFO @ Sat, 15 Jan 2022 19:16:20: 20000000 INFO @ Sat, 15 Jan 2022 19:16:20: 12000000 INFO @ Sat, 15 Jan 2022 19:16:23: 17000000 INFO @ Sat, 15 Jan 2022 19:16:27: 21000000 INFO @ Sat, 15 Jan 2022 19:16:27: 13000000 INFO @ Sat, 15 Jan 2022 19:16:30: 18000000 INFO @ Sat, 15 Jan 2022 19:16:34: 14000000 INFO @ Sat, 15 Jan 2022 19:16:34: 22000000 INFO @ Sat, 15 Jan 2022 19:16:37: 19000000 INFO @ Sat, 15 Jan 2022 19:16:41: 15000000 INFO @ Sat, 15 Jan 2022 19:16:42: 23000000 INFO @ Sat, 15 Jan 2022 19:16:43: 20000000 INFO @ Sat, 15 Jan 2022 19:16:48: 16000000 INFO @ Sat, 15 Jan 2022 19:16:49: 24000000 INFO @ Sat, 15 Jan 2022 19:16:50: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:16:55: 17000000 INFO @ Sat, 15 Jan 2022 19:16:56: 25000000 INFO @ Sat, 15 Jan 2022 19:16:57: 22000000 INFO @ Sat, 15 Jan 2022 19:17:02: 18000000 INFO @ Sat, 15 Jan 2022 19:17:03: 26000000 INFO @ Sat, 15 Jan 2022 19:17:04: 23000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:17:08: 19000000 INFO @ Sat, 15 Jan 2022 19:17:11: 27000000 INFO @ Sat, 15 Jan 2022 19:17:11: 24000000 INFO @ Sat, 15 Jan 2022 19:17:15: 20000000 INFO @ Sat, 15 Jan 2022 19:17:18: 25000000 INFO @ Sat, 15 Jan 2022 19:17:18: 28000000 INFO @ Sat, 15 Jan 2022 19:17:22: 21000000 INFO @ Sat, 15 Jan 2022 19:17:24: 26000000 INFO @ Sat, 15 Jan 2022 19:17:25: 29000000 INFO @ Sat, 15 Jan 2022 19:17:29: 22000000 INFO @ Sat, 15 Jan 2022 19:17:31: 27000000 INFO @ Sat, 15 Jan 2022 19:17:32: 30000000 INFO @ Sat, 15 Jan 2022 19:17:36: 23000000 INFO @ Sat, 15 Jan 2022 19:17:38: 28000000 INFO @ Sat, 15 Jan 2022 19:17:39: 31000000 INFO @ Sat, 15 Jan 2022 19:17:42: 24000000 INFO @ Sat, 15 Jan 2022 19:17:45: 29000000 INFO @ Sat, 15 Jan 2022 19:17:47: 32000000 INFO @ Sat, 15 Jan 2022 19:17:49: 25000000 INFO @ Sat, 15 Jan 2022 19:17:52: 30000000 INFO @ Sat, 15 Jan 2022 19:17:54: 33000000 INFO @ Sat, 15 Jan 2022 19:17:56: 26000000 INFO @ Sat, 15 Jan 2022 19:17:58: 31000000 INFO @ Sat, 15 Jan 2022 19:18:01: 34000000 INFO @ Sat, 15 Jan 2022 19:18:03: 27000000 INFO @ Sat, 15 Jan 2022 19:18:05: 32000000 INFO @ Sat, 15 Jan 2022 19:18:08: 35000000 INFO @ Sat, 15 Jan 2022 19:18:10: 28000000 INFO @ Sat, 15 Jan 2022 19:18:12: 33000000 INFO @ Sat, 15 Jan 2022 19:18:15: 36000000 INFO @ Sat, 15 Jan 2022 19:18:16: 29000000 INFO @ Sat, 15 Jan 2022 19:18:19: 34000000 INFO @ Sat, 15 Jan 2022 19:18:23: 37000000 INFO @ Sat, 15 Jan 2022 19:18:23: 30000000 INFO @ Sat, 15 Jan 2022 19:18:25: 35000000 INFO @ Sat, 15 Jan 2022 19:18:30: 38000000 INFO @ Sat, 15 Jan 2022 19:18:30: 31000000 INFO @ Sat, 15 Jan 2022 19:18:32: 36000000 INFO @ Sat, 15 Jan 2022 19:18:37: 39000000 INFO @ Sat, 15 Jan 2022 19:18:37: 32000000 INFO @ Sat, 15 Jan 2022 19:18:39: 37000000 INFO @ Sat, 15 Jan 2022 19:18:44: 33000000 INFO @ Sat, 15 Jan 2022 19:18:44: 40000000 INFO @ Sat, 15 Jan 2022 19:18:46: 38000000 INFO @ Sat, 15 Jan 2022 19:18:50: 34000000 INFO @ Sat, 15 Jan 2022 19:18:51: 41000000 INFO @ Sat, 15 Jan 2022 19:18:53: 39000000 INFO @ Sat, 15 Jan 2022 19:18:57: 35000000 INFO @ Sat, 15 Jan 2022 19:18:58: 42000000 INFO @ Sat, 15 Jan 2022 19:18:59: 40000000 INFO @ Sat, 15 Jan 2022 19:19:04: 36000000 INFO @ Sat, 15 Jan 2022 19:19:05: 43000000 INFO @ Sat, 15 Jan 2022 19:19:06: 41000000 INFO @ Sat, 15 Jan 2022 19:19:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:19:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:19:06: #1 total tags in treatment: 19995134 INFO @ Sat, 15 Jan 2022 19:19:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:07: #1 tags after filtering in treatment: 7723578 INFO @ Sat, 15 Jan 2022 19:19:07: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 19:19:07: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:19:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:19:10: 37000000 INFO @ Sat, 15 Jan 2022 19:19:12: 42000000 INFO @ Sat, 15 Jan 2022 19:19:17: 38000000 INFO @ Sat, 15 Jan 2022 19:19:18: 43000000 INFO @ Sat, 15 Jan 2022 19:19:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:19:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:19:20: #1 total tags in treatment: 19995134 INFO @ Sat, 15 Jan 2022 19:19:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:20: #1 tags after filtering in treatment: 7723578 INFO @ Sat, 15 Jan 2022 19:19:20: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 19:19:20: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:19:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:19:23: 39000000 INFO @ Sat, 15 Jan 2022 19:19:28: 40000000 INFO @ Sat, 15 Jan 2022 19:19:33: 41000000 INFO @ Sat, 15 Jan 2022 19:19:38: 42000000 INFO @ Sat, 15 Jan 2022 19:19:43: 43000000 INFO @ Sat, 15 Jan 2022 19:19:44: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:19:44: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:19:44: #1 total tags in treatment: 19995134 INFO @ Sat, 15 Jan 2022 19:19:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:45: #1 tags after filtering in treatment: 7723578 INFO @ Sat, 15 Jan 2022 19:19:45: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 15 Jan 2022 19:19:45: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:19:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10701689/SRX10701689.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling