Job ID = 2009702 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,080,673 reads read : 12,080,673 reads written : 12,080,673 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:25 12080673 reads; of these: 12080673 (100.00%) were unpaired; of these: 813373 (6.73%) aligned 0 times 9782224 (80.97%) aligned exactly 1 time 1485076 (12.29%) aligned >1 times 93.27% overall alignment rate Time searching: 00:01:25 Overall time: 00:01:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3463668 / 11267300 = 0.3074 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:36:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:36:25: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:36:25: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:36:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:36:26: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:36:26: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:36:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:36:27: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:36:27: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:36:31: 1000000 INFO @ Fri, 05 Jul 2019 19:36:32: 1000000 INFO @ Fri, 05 Jul 2019 19:36:34: 1000000 INFO @ Fri, 05 Jul 2019 19:36:36: 2000000 INFO @ Fri, 05 Jul 2019 19:36:39: 2000000 INFO @ Fri, 05 Jul 2019 19:36:41: 2000000 INFO @ Fri, 05 Jul 2019 19:36:42: 3000000 INFO @ Fri, 05 Jul 2019 19:36:45: 3000000 INFO @ Fri, 05 Jul 2019 19:36:47: 3000000 INFO @ Fri, 05 Jul 2019 19:36:47: 4000000 INFO @ Fri, 05 Jul 2019 19:36:51: 4000000 INFO @ Fri, 05 Jul 2019 19:36:53: 5000000 INFO @ Fri, 05 Jul 2019 19:36:54: 4000000 INFO @ Fri, 05 Jul 2019 19:36:57: 5000000 INFO @ Fri, 05 Jul 2019 19:36:59: 6000000 INFO @ Fri, 05 Jul 2019 19:37:01: 5000000 INFO @ Fri, 05 Jul 2019 19:37:03: 6000000 INFO @ Fri, 05 Jul 2019 19:37:04: 7000000 INFO @ Fri, 05 Jul 2019 19:37:07: 6000000 INFO @ Fri, 05 Jul 2019 19:37:09: #1 tag size is determined as 32 bps INFO @ Fri, 05 Jul 2019 19:37:09: #1 tag size = 32 INFO @ Fri, 05 Jul 2019 19:37:09: #1 total tags in treatment: 7803632 INFO @ Fri, 05 Jul 2019 19:37:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:09: #1 tags after filtering in treatment: 7803632 INFO @ Fri, 05 Jul 2019 19:37:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:09: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:09: 7000000 INFO @ Fri, 05 Jul 2019 19:37:10: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:37:14: 7000000 INFO @ Fri, 05 Jul 2019 19:37:14: #1 tag size is determined as 32 bps INFO @ Fri, 05 Jul 2019 19:37:14: #1 tag size = 32 INFO @ Fri, 05 Jul 2019 19:37:14: #1 total tags in treatment: 7803632 INFO @ Fri, 05 Jul 2019 19:37:14: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:14: #1 tags after filtering in treatment: 7803632 INFO @ Fri, 05 Jul 2019 19:37:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:14: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:14: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:15: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:37:19: #1 tag size is determined as 32 bps INFO @ Fri, 05 Jul 2019 19:37:19: #1 tag size = 32 INFO @ Fri, 05 Jul 2019 19:37:19: #1 total tags in treatment: 7803632 INFO @ Fri, 05 Jul 2019 19:37:19: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:19: #1 tags after filtering in treatment: 7803632 INFO @ Fri, 05 Jul 2019 19:37:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:19: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:19: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:20: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067732/SRX1067732.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。