Job ID = 2009701 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,461,823 reads read : 7,461,823 reads written : 7,461,823 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:57 7461823 reads; of these: 7461823 (100.00%) were unpaired; of these: 508261 (6.81%) aligned 0 times 6092900 (81.65%) aligned exactly 1 time 860662 (11.53%) aligned >1 times 93.19% overall alignment rate Time searching: 00:01:57 Overall time: 00:01:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1899579 / 6953562 = 0.2732 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:37:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:10: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:10: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:37:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:11: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:11: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:37:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:37:18: 1000000 INFO @ Fri, 05 Jul 2019 19:37:20: 1000000 INFO @ Fri, 05 Jul 2019 19:37:22: 1000000 INFO @ Fri, 05 Jul 2019 19:37:25: 2000000 INFO @ Fri, 05 Jul 2019 19:37:28: 2000000 INFO @ Fri, 05 Jul 2019 19:37:28: 2000000 INFO @ Fri, 05 Jul 2019 19:37:32: 3000000 INFO @ Fri, 05 Jul 2019 19:37:35: 3000000 INFO @ Fri, 05 Jul 2019 19:37:37: 3000000 INFO @ Fri, 05 Jul 2019 19:37:39: 4000000 INFO @ Fri, 05 Jul 2019 19:37:41: 4000000 INFO @ Fri, 05 Jul 2019 19:37:45: 4000000 INFO @ Fri, 05 Jul 2019 19:37:46: 5000000 INFO @ Fri, 05 Jul 2019 19:37:47: 5000000 INFO @ Fri, 05 Jul 2019 19:37:48: #1 tag size is determined as 32 bps INFO @ Fri, 05 Jul 2019 19:37:48: #1 tag size = 32 INFO @ Fri, 05 Jul 2019 19:37:48: #1 total tags in treatment: 5053983 INFO @ Fri, 05 Jul 2019 19:37:48: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:48: #1 tag size is determined as 32 bps INFO @ Fri, 05 Jul 2019 19:37:48: #1 tag size = 32 INFO @ Fri, 05 Jul 2019 19:37:48: #1 total tags in treatment: 5053983 INFO @ Fri, 05 Jul 2019 19:37:48: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:48: #1 tags after filtering in treatment: 5053983 INFO @ Fri, 05 Jul 2019 19:37:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:48: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:48: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:48: #1 tags after filtering in treatment: 5053983 INFO @ Fri, 05 Jul 2019 19:37:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:48: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:48: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:49: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:49: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:37:49: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:37:52: 5000000 INFO @ Fri, 05 Jul 2019 19:37:53: #1 tag size is determined as 32 bps INFO @ Fri, 05 Jul 2019 19:37:53: #1 tag size = 32 INFO @ Fri, 05 Jul 2019 19:37:53: #1 total tags in treatment: 5053983 INFO @ Fri, 05 Jul 2019 19:37:53: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:53: #1 tags after filtering in treatment: 5053983 INFO @ Fri, 05 Jul 2019 19:37:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:53: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:53: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:53: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1067731/SRX1067731.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。