Job ID = 9161822 sra ファイルのダウンロード中... Completed: 564499K bytes transferred in 7 seconds (635919K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 18097567 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1066352/SRR2071184.sra Written 18097567 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:25 18097567 reads; of these: 18097567 (100.00%) were unpaired; of these: 1759545 (9.72%) aligned 0 times 14359437 (79.34%) aligned exactly 1 time 1978585 (10.93%) aligned >1 times 90.28% overall alignment rate Time searching: 00:03:25 Overall time: 00:03:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6620028 / 16338022 = 0.4052 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:48:32: # Command line: callpeak -t SRX1066352.bam -f BAM -g 12100000 -n SRX1066352.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1066352.05 # format = BAM # ChIP-seq file = ['SRX1066352.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:48:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:48:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:48:32: # Command line: callpeak -t SRX1066352.bam -f BAM -g 12100000 -n SRX1066352.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1066352.10 # format = BAM # ChIP-seq file = ['SRX1066352.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:48:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:48:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:48:32: # Command line: callpeak -t SRX1066352.bam -f BAM -g 12100000 -n SRX1066352.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1066352.20 # format = BAM # ChIP-seq file = ['SRX1066352.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:48:32: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:48:32: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:48:40: 1000000 INFO @ Wed, 28 Jun 2017 04:48:40: 1000000 INFO @ Wed, 28 Jun 2017 04:48:41: 1000000 INFO @ Wed, 28 Jun 2017 04:48:48: 2000000 INFO @ Wed, 28 Jun 2017 04:48:48: 2000000 INFO @ Wed, 28 Jun 2017 04:48:49: 2000000 INFO @ Wed, 28 Jun 2017 04:48:56: 3000000 INFO @ Wed, 28 Jun 2017 04:48:56: 3000000 INFO @ Wed, 28 Jun 2017 04:48:57: 3000000 INFO @ Wed, 28 Jun 2017 04:49:03: 4000000 INFO @ Wed, 28 Jun 2017 04:49:03: 4000000 INFO @ Wed, 28 Jun 2017 04:49:05: 4000000 INFO @ Wed, 28 Jun 2017 04:49:11: 5000000 INFO @ Wed, 28 Jun 2017 04:49:11: 5000000 INFO @ Wed, 28 Jun 2017 04:49:13: 5000000 INFO @ Wed, 28 Jun 2017 04:49:20: 6000000 INFO @ Wed, 28 Jun 2017 04:49:20: 6000000 INFO @ Wed, 28 Jun 2017 04:49:22: 6000000 INFO @ Wed, 28 Jun 2017 04:49:28: 7000000 INFO @ Wed, 28 Jun 2017 04:49:28: 7000000 INFO @ Wed, 28 Jun 2017 04:49:30: 7000000 INFO @ Wed, 28 Jun 2017 04:49:36: 8000000 INFO @ Wed, 28 Jun 2017 04:49:36: 8000000 INFO @ Wed, 28 Jun 2017 04:49:38: 8000000 INFO @ Wed, 28 Jun 2017 04:49:44: 9000000 INFO @ Wed, 28 Jun 2017 04:49:44: 9000000 INFO @ Wed, 28 Jun 2017 04:49:46: 9000000 INFO @ Wed, 28 Jun 2017 04:49:49: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:49:49: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:49:49: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:49:49: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:49:49: #1 total tags in treatment: 9717994 INFO @ Wed, 28 Jun 2017 04:49:49: #1 total tags in treatment: 9717994 INFO @ Wed, 28 Jun 2017 04:49:49: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:49:49: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:49:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:49:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:49:49: #1 tags after filtering in treatment: 9717994 INFO @ Wed, 28 Jun 2017 04:49:49: #1 tags after filtering in treatment: 9717994 INFO @ Wed, 28 Jun 2017 04:49:49: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:49:49: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:49:49: #1 finished! INFO @ Wed, 28 Jun 2017 04:49:49: #1 finished! INFO @ Wed, 28 Jun 2017 04:49:49: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:49:49: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:49:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:49:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:49:50: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:49:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:49:50: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 04:49:50: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:49:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:49:50: Process for pairing-model is terminated! cat: SRX1066352.05_peaks.narrowPeakcat: SRX1066352.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1066352.05_model.r'rm: cannot remove `SRX1066352.10_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066352.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066352.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066352.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066352.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:49:51: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:49:51: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:49:51: #1 total tags in treatment: 9717994 INFO @ Wed, 28 Jun 2017 04:49:51: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:49:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:49:51: #1 tags after filtering in treatment: 9717994 INFO @ Wed, 28 Jun 2017 04:49:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:49:51: #1 finished! INFO @ Wed, 28 Jun 2017 04:49:51: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:49:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:49:52: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:49:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:49:52: Process for pairing-model is terminated! cat: SRX1066352.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1066352.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066352.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066352.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。