Job ID = 9161819 sra ファイルのダウンロード中... Completed: 622720K bytes transferred in 8 seconds (617402K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20002403 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1066349/SRR2071181.sra Written 20002403 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:32 20002403 reads; of these: 20002403 (100.00%) were unpaired; of these: 1747101 (8.73%) aligned 0 times 15562294 (77.80%) aligned exactly 1 time 2693008 (13.46%) aligned >1 times 91.27% overall alignment rate Time searching: 00:03:32 Overall time: 00:03:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7564483 / 18255302 = 0.4144 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:47:43: # Command line: callpeak -t SRX1066349.bam -f BAM -g 12100000 -n SRX1066349.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1066349.20 # format = BAM # ChIP-seq file = ['SRX1066349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:47:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:47:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:47:43: # Command line: callpeak -t SRX1066349.bam -f BAM -g 12100000 -n SRX1066349.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1066349.10 # format = BAM # ChIP-seq file = ['SRX1066349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:47:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:47:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:47:43: # Command line: callpeak -t SRX1066349.bam -f BAM -g 12100000 -n SRX1066349.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1066349.05 # format = BAM # ChIP-seq file = ['SRX1066349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:47:43: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:47:43: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:47:50: 1000000 INFO @ Wed, 28 Jun 2017 04:47:50: 1000000 INFO @ Wed, 28 Jun 2017 04:47:51: 1000000 INFO @ Wed, 28 Jun 2017 04:47:57: 2000000 INFO @ Wed, 28 Jun 2017 04:47:57: 2000000 INFO @ Wed, 28 Jun 2017 04:47:58: 2000000 INFO @ Wed, 28 Jun 2017 04:48:04: 3000000 INFO @ Wed, 28 Jun 2017 04:48:04: 3000000 INFO @ Wed, 28 Jun 2017 04:48:04: 3000000 INFO @ Wed, 28 Jun 2017 04:48:11: 4000000 INFO @ Wed, 28 Jun 2017 04:48:11: 4000000 INFO @ Wed, 28 Jun 2017 04:48:11: 4000000 INFO @ Wed, 28 Jun 2017 04:48:18: 5000000 INFO @ Wed, 28 Jun 2017 04:48:18: 5000000 INFO @ Wed, 28 Jun 2017 04:48:18: 5000000 INFO @ Wed, 28 Jun 2017 04:48:25: 6000000 INFO @ Wed, 28 Jun 2017 04:48:25: 6000000 INFO @ Wed, 28 Jun 2017 04:48:26: 6000000 INFO @ Wed, 28 Jun 2017 04:48:31: 7000000 INFO @ Wed, 28 Jun 2017 04:48:31: 7000000 INFO @ Wed, 28 Jun 2017 04:48:33: 7000000 INFO @ Wed, 28 Jun 2017 04:48:38: 8000000 INFO @ Wed, 28 Jun 2017 04:48:38: 8000000 INFO @ Wed, 28 Jun 2017 04:48:40: 8000000 INFO @ Wed, 28 Jun 2017 04:48:45: 9000000 INFO @ Wed, 28 Jun 2017 04:48:45: 9000000 INFO @ Wed, 28 Jun 2017 04:48:47: 9000000 INFO @ Wed, 28 Jun 2017 04:48:52: 10000000 INFO @ Wed, 28 Jun 2017 04:48:52: 10000000 INFO @ Wed, 28 Jun 2017 04:48:54: 10000000 INFO @ Wed, 28 Jun 2017 04:48:56: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:48:56: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:48:56: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:48:56: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:48:56: #1 total tags in treatment: 10690819 INFO @ Wed, 28 Jun 2017 04:48:56: #1 total tags in treatment: 10690819 INFO @ Wed, 28 Jun 2017 04:48:56: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:48:56: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:48:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:48:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:48:56: #1 tags after filtering in treatment: 10690819 INFO @ Wed, 28 Jun 2017 04:48:56: #1 tags after filtering in treatment: 10690819 INFO @ Wed, 28 Jun 2017 04:48:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:48:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:48:56: #1 finished! INFO @ Wed, 28 Jun 2017 04:48:56: #1 finished! INFO @ Wed, 28 Jun 2017 04:48:56: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:48:56: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:48:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:48:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:48:57: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:48:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:48:57: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 04:48:57: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:48:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:48:57: Process for pairing-model is terminated! cat: SRX1066349.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX1066349.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1066349.10_model.r'pass1 - making usageList (0 chroms): 1 millis : そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066349.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066349.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove `SRX1066349.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066349.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066349.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:48:59: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:48:59: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:48:59: #1 total tags in treatment: 10690819 INFO @ Wed, 28 Jun 2017 04:48:59: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:48:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:48:59: #1 tags after filtering in treatment: 10690819 INFO @ Wed, 28 Jun 2017 04:48:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:48:59: #1 finished! INFO @ Wed, 28 Jun 2017 04:48:59: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:48:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:48:59: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:48:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:48:59: Process for pairing-model is terminated! cat: SRX1066349.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1066349.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066349.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066349.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。