Job ID = 9161818 sra ファイルのダウンロード中... Completed: 575892K bytes transferred in 7 seconds (639532K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 18556675 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1066342/SRR2071174.sra Written 18556675 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:55 18556675 reads; of these: 18556675 (100.00%) were unpaired; of these: 940823 (5.07%) aligned 0 times 15222814 (82.03%) aligned exactly 1 time 2393038 (12.90%) aligned >1 times 94.93% overall alignment rate Time searching: 00:03:56 Overall time: 00:03:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8444032 / 17615852 = 0.4793 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 04:47:41: # Command line: callpeak -t SRX1066342.bam -f BAM -g 12100000 -n SRX1066342.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1066342.10 # format = BAM # ChIP-seq file = ['SRX1066342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:47:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:47:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:47:41: # Command line: callpeak -t SRX1066342.bam -f BAM -g 12100000 -n SRX1066342.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1066342.05 # format = BAM # ChIP-seq file = ['SRX1066342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:47:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:47:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:47:41: # Command line: callpeak -t SRX1066342.bam -f BAM -g 12100000 -n SRX1066342.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1066342.20 # format = BAM # ChIP-seq file = ['SRX1066342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 04:47:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 04:47:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 04:47:48: 1000000 INFO @ Wed, 28 Jun 2017 04:47:48: 1000000 INFO @ Wed, 28 Jun 2017 04:47:48: 1000000 INFO @ Wed, 28 Jun 2017 04:47:54: 2000000 INFO @ Wed, 28 Jun 2017 04:47:54: 2000000 INFO @ Wed, 28 Jun 2017 04:47:55: 2000000 INFO @ Wed, 28 Jun 2017 04:48:01: 3000000 INFO @ Wed, 28 Jun 2017 04:48:01: 3000000 INFO @ Wed, 28 Jun 2017 04:48:01: 3000000 INFO @ Wed, 28 Jun 2017 04:48:07: 4000000 INFO @ Wed, 28 Jun 2017 04:48:07: 4000000 INFO @ Wed, 28 Jun 2017 04:48:08: 4000000 INFO @ Wed, 28 Jun 2017 04:48:13: 5000000 INFO @ Wed, 28 Jun 2017 04:48:14: 5000000 INFO @ Wed, 28 Jun 2017 04:48:15: 5000000 INFO @ Wed, 28 Jun 2017 04:48:20: 6000000 INFO @ Wed, 28 Jun 2017 04:48:21: 6000000 INFO @ Wed, 28 Jun 2017 04:48:22: 6000000 INFO @ Wed, 28 Jun 2017 04:48:26: 7000000 INFO @ Wed, 28 Jun 2017 04:48:27: 7000000 INFO @ Wed, 28 Jun 2017 04:48:29: 7000000 INFO @ Wed, 28 Jun 2017 04:48:32: 8000000 INFO @ Wed, 28 Jun 2017 04:48:34: 8000000 INFO @ Wed, 28 Jun 2017 04:48:36: 8000000 INFO @ Wed, 28 Jun 2017 04:48:38: 9000000 INFO @ Wed, 28 Jun 2017 04:48:40: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:48:40: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:48:40: #1 total tags in treatment: 9171820 INFO @ Wed, 28 Jun 2017 04:48:40: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:48:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:48:40: #1 tags after filtering in treatment: 9171820 INFO @ Wed, 28 Jun 2017 04:48:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:48:40: #1 finished! INFO @ Wed, 28 Jun 2017 04:48:40: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:48:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:48:40: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:48:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:48:40: Process for pairing-model is terminated! cat: SRX1066342.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1066342.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066342.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066342.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:48:41: 9000000 INFO @ Wed, 28 Jun 2017 04:48:42: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:48:42: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:48:42: #1 total tags in treatment: 9171820 INFO @ Wed, 28 Jun 2017 04:48:42: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:48:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:48:42: #1 tags after filtering in treatment: 9171820 INFO @ Wed, 28 Jun 2017 04:48:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:48:42: #1 finished! INFO @ Wed, 28 Jun 2017 04:48:42: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:48:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:48:43: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:48:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:48:43: Process for pairing-model is terminated! cat: SRX1066342.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1066342.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066342.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066342.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 04:48:43: 9000000 INFO @ Wed, 28 Jun 2017 04:48:44: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 04:48:44: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 04:48:44: #1 total tags in treatment: 9171820 INFO @ Wed, 28 Jun 2017 04:48:44: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 04:48:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 04:48:44: #1 tags after filtering in treatment: 9171820 INFO @ Wed, 28 Jun 2017 04:48:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 04:48:44: #1 finished! INFO @ Wed, 28 Jun 2017 04:48:44: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 04:48:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 04:48:45: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 04:48:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 04:48:45: Process for pairing-model is terminated! cat: SRX1066342.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1066342.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066342.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1066342.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。