Job ID = 14520189 SRX = SRX10592382 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:32:20 prefetch.2.10.7: 1) Downloading 'SRR14227542'... 2022-01-15T09:32:20 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:33:21 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:33:21 prefetch.2.10.7: 1) 'SRR14227542' was downloaded successfully 2022-01-15T09:33:21 prefetch.2.10.7: 'SRR14227542' has 0 unresolved dependencies Read 27368043 spots for SRR14227542/SRR14227542.sra Written 27368043 spots for SRR14227542/SRR14227542.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:58 27368043 reads; of these: 27368043 (100.00%) were paired; of these: 1717944 (6.28%) aligned concordantly 0 times 19408872 (70.92%) aligned concordantly exactly 1 time 6241227 (22.80%) aligned concordantly >1 times ---- 1717944 pairs aligned concordantly 0 times; of these: 64819 (3.77%) aligned discordantly 1 time ---- 1653125 pairs aligned 0 times concordantly or discordantly; of these: 3306250 mates make up the pairs; of these: 2654150 (80.28%) aligned 0 times 506686 (15.33%) aligned exactly 1 time 145414 (4.40%) aligned >1 times 95.15% overall alignment rate Time searching: 00:23:58 Overall time: 00:23:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 14887538 / 25710170 = 0.5791 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:19: 1000000 INFO @ Sat, 15 Jan 2022 19:15:29: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:15:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:15:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:15:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:15:39: 3000000 INFO @ Sat, 15 Jan 2022 19:15:48: 1000000 INFO @ Sat, 15 Jan 2022 19:15:49: 4000000 INFO @ Sat, 15 Jan 2022 19:15:58: 2000000 INFO @ Sat, 15 Jan 2022 19:16:00: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:16:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:16:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:16:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:16:08: 3000000 INFO @ Sat, 15 Jan 2022 19:16:10: 6000000 INFO @ Sat, 15 Jan 2022 19:16:17: 1000000 INFO @ Sat, 15 Jan 2022 19:16:18: 4000000 INFO @ Sat, 15 Jan 2022 19:16:21: 7000000 INFO @ Sat, 15 Jan 2022 19:16:27: 2000000 INFO @ Sat, 15 Jan 2022 19:16:28: 5000000 INFO @ Sat, 15 Jan 2022 19:16:31: 8000000 INFO @ Sat, 15 Jan 2022 19:16:37: 3000000 INFO @ Sat, 15 Jan 2022 19:16:39: 6000000 INFO @ Sat, 15 Jan 2022 19:16:41: 9000000 INFO @ Sat, 15 Jan 2022 19:16:47: 4000000 INFO @ Sat, 15 Jan 2022 19:16:49: 7000000 INFO @ Sat, 15 Jan 2022 19:16:52: 10000000 INFO @ Sat, 15 Jan 2022 19:16:57: 5000000 INFO @ Sat, 15 Jan 2022 19:17:00: 8000000 INFO @ Sat, 15 Jan 2022 19:17:03: 11000000 INFO @ Sat, 15 Jan 2022 19:17:07: 6000000 INFO @ Sat, 15 Jan 2022 19:17:10: 9000000 INFO @ Sat, 15 Jan 2022 19:17:14: 12000000 INFO @ Sat, 15 Jan 2022 19:17:17: 7000000 INFO @ Sat, 15 Jan 2022 19:17:20: 10000000 INFO @ Sat, 15 Jan 2022 19:17:25: 13000000 INFO @ Sat, 15 Jan 2022 19:17:27: 8000000 INFO @ Sat, 15 Jan 2022 19:17:31: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:17:36: 14000000 INFO @ Sat, 15 Jan 2022 19:17:37: 9000000 INFO @ Sat, 15 Jan 2022 19:17:41: 12000000 INFO @ Sat, 15 Jan 2022 19:17:47: 15000000 INFO @ Sat, 15 Jan 2022 19:17:48: 10000000 INFO @ Sat, 15 Jan 2022 19:17:50: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:17:58: 11000000 INFO @ Sat, 15 Jan 2022 19:17:58: 16000000 INFO @ Sat, 15 Jan 2022 19:18:00: 14000000 INFO @ Sat, 15 Jan 2022 19:18:08: 12000000 INFO @ Sat, 15 Jan 2022 19:18:10: 17000000 INFO @ Sat, 15 Jan 2022 19:18:10: 15000000 INFO @ Sat, 15 Jan 2022 19:18:17: 13000000 INFO @ Sat, 15 Jan 2022 19:18:19: 16000000 INFO @ Sat, 15 Jan 2022 19:18:20: 18000000 INFO @ Sat, 15 Jan 2022 19:18:27: 14000000 INFO @ Sat, 15 Jan 2022 19:18:29: 17000000 INFO @ Sat, 15 Jan 2022 19:18:31: 19000000 INFO @ Sat, 15 Jan 2022 19:18:37: 15000000 INFO @ Sat, 15 Jan 2022 19:18:40: 18000000 INFO @ Sat, 15 Jan 2022 19:18:42: 20000000 INFO @ Sat, 15 Jan 2022 19:18:46: 16000000 INFO @ Sat, 15 Jan 2022 19:18:50: 19000000 INFO @ Sat, 15 Jan 2022 19:18:53: 21000000 INFO @ Sat, 15 Jan 2022 19:18:56: 17000000 INFO @ Sat, 15 Jan 2022 19:19:00: 20000000 INFO @ Sat, 15 Jan 2022 19:19:04: 22000000 INFO @ Sat, 15 Jan 2022 19:19:06: 18000000 INFO @ Sat, 15 Jan 2022 19:19:07: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:19:07: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:19:07: #1 total tags in treatment: 10794780 INFO @ Sat, 15 Jan 2022 19:19:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:07: #1 tags after filtering in treatment: 7161694 INFO @ Sat, 15 Jan 2022 19:19:07: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 19:19:07: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:19:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:19:10: 21000000 INFO @ Sat, 15 Jan 2022 19:19:16: 19000000 INFO @ Sat, 15 Jan 2022 19:19:19: 22000000 INFO @ Sat, 15 Jan 2022 19:19:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:19:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:19:22: #1 total tags in treatment: 10794780 INFO @ Sat, 15 Jan 2022 19:19:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:22: #1 tags after filtering in treatment: 7161694 INFO @ Sat, 15 Jan 2022 19:19:22: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 19:19:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:19:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:19:26: 20000000 INFO @ Sat, 15 Jan 2022 19:19:35: 21000000 INFO @ Sat, 15 Jan 2022 19:19:44: 22000000 INFO @ Sat, 15 Jan 2022 19:19:46: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:19:46: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:19:46: #1 total tags in treatment: 10794780 INFO @ Sat, 15 Jan 2022 19:19:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:47: #1 tags after filtering in treatment: 7161694 INFO @ Sat, 15 Jan 2022 19:19:47: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 19:19:47: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:19:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592382/SRX10592382.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling