Job ID = 14520188 SRX = SRX10592381 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:32:06 prefetch.2.10.7: 1) Downloading 'SRR14227541'... 2022-01-15T09:32:06 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:33:17 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:33:17 prefetch.2.10.7: 1) 'SRR14227541' was downloaded successfully 2022-01-15T09:33:17 prefetch.2.10.7: 'SRR14227541' has 0 unresolved dependencies Read 25472461 spots for SRR14227541/SRR14227541.sra Written 25472461 spots for SRR14227541/SRR14227541.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:26 25472461 reads; of these: 25472461 (100.00%) were paired; of these: 964008 (3.78%) aligned concordantly 0 times 19616747 (77.01%) aligned concordantly exactly 1 time 4891706 (19.20%) aligned concordantly >1 times ---- 964008 pairs aligned concordantly 0 times; of these: 46942 (4.87%) aligned discordantly 1 time ---- 917066 pairs aligned 0 times concordantly or discordantly; of these: 1834132 mates make up the pairs; of these: 1328409 (72.43%) aligned 0 times 376690 (20.54%) aligned exactly 1 time 129033 (7.04%) aligned >1 times 97.39% overall alignment rate Time searching: 00:15:26 Overall time: 00:15:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 7194430 / 24549863 = 0.2931 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:00:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:00:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:00:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:00:13: 1000000 INFO @ Sat, 15 Jan 2022 19:00:19: 2000000 INFO @ Sat, 15 Jan 2022 19:00:25: 3000000 INFO @ Sat, 15 Jan 2022 19:00:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:00:37: 5000000 INFO @ Sat, 15 Jan 2022 19:00:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:00:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:00:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:00:43: 6000000 INFO @ Sat, 15 Jan 2022 19:00:44: 1000000 INFO @ Sat, 15 Jan 2022 19:00:49: 7000000 INFO @ Sat, 15 Jan 2022 19:00:51: 2000000 INFO @ Sat, 15 Jan 2022 19:00:55: 8000000 INFO @ Sat, 15 Jan 2022 19:00:57: 3000000 INFO @ Sat, 15 Jan 2022 19:01:02: 9000000 INFO @ Sat, 15 Jan 2022 19:01:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:01:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:01:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:01:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:01:08: 10000000 INFO @ Sat, 15 Jan 2022 19:01:10: 5000000 INFO @ Sat, 15 Jan 2022 19:01:14: 11000000 INFO @ Sat, 15 Jan 2022 19:01:14: 1000000 INFO @ Sat, 15 Jan 2022 19:01:17: 6000000 INFO @ Sat, 15 Jan 2022 19:01:21: 12000000 INFO @ Sat, 15 Jan 2022 19:01:21: 2000000 INFO @ Sat, 15 Jan 2022 19:01:24: 7000000 INFO @ Sat, 15 Jan 2022 19:01:27: 13000000 INFO @ Sat, 15 Jan 2022 19:01:28: 3000000 INFO @ Sat, 15 Jan 2022 19:01:30: 8000000 INFO @ Sat, 15 Jan 2022 19:01:33: 14000000 INFO @ Sat, 15 Jan 2022 19:01:34: 4000000 INFO @ Sat, 15 Jan 2022 19:01:37: 9000000 INFO @ Sat, 15 Jan 2022 19:01:40: 15000000 INFO @ Sat, 15 Jan 2022 19:01:40: 5000000 INFO @ Sat, 15 Jan 2022 19:01:43: 10000000 INFO @ Sat, 15 Jan 2022 19:01:46: 16000000 INFO @ Sat, 15 Jan 2022 19:01:47: 6000000 INFO @ Sat, 15 Jan 2022 19:01:50: 11000000 INFO @ Sat, 15 Jan 2022 19:01:53: 17000000 INFO @ Sat, 15 Jan 2022 19:01:53: 7000000 INFO @ Sat, 15 Jan 2022 19:01:56: 12000000 INFO @ Sat, 15 Jan 2022 19:01:59: 18000000 INFO @ Sat, 15 Jan 2022 19:02:00: 8000000 INFO @ Sat, 15 Jan 2022 19:02:03: 13000000 INFO @ Sat, 15 Jan 2022 19:02:05: 19000000 INFO @ Sat, 15 Jan 2022 19:02:06: 9000000 INFO @ Sat, 15 Jan 2022 19:02:10: 14000000 INFO @ Sat, 15 Jan 2022 19:02:12: 20000000 INFO @ Sat, 15 Jan 2022 19:02:13: 10000000 INFO @ Sat, 15 Jan 2022 19:02:16: 15000000 INFO @ Sat, 15 Jan 2022 19:02:18: 21000000 INFO @ Sat, 15 Jan 2022 19:02:19: 11000000 INFO @ Sat, 15 Jan 2022 19:02:23: 16000000 INFO @ Sat, 15 Jan 2022 19:02:24: 22000000 INFO @ Sat, 15 Jan 2022 19:02:26: 12000000 INFO @ Sat, 15 Jan 2022 19:02:29: 17000000 INFO @ Sat, 15 Jan 2022 19:02:31: 23000000 INFO @ Sat, 15 Jan 2022 19:02:32: 13000000 INFO @ Sat, 15 Jan 2022 19:02:36: 18000000 INFO @ Sat, 15 Jan 2022 19:02:37: 24000000 INFO @ Sat, 15 Jan 2022 19:02:39: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:02:42: 19000000 INFO @ Sat, 15 Jan 2022 19:02:44: 25000000 INFO @ Sat, 15 Jan 2022 19:02:45: 15000000 INFO @ Sat, 15 Jan 2022 19:02:49: 20000000 INFO @ Sat, 15 Jan 2022 19:02:50: 26000000 INFO @ Sat, 15 Jan 2022 19:02:51: 16000000 INFO @ Sat, 15 Jan 2022 19:02:55: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:02:57: 27000000 INFO @ Sat, 15 Jan 2022 19:02:58: 17000000 INFO @ Sat, 15 Jan 2022 19:03:02: 22000000 INFO @ Sat, 15 Jan 2022 19:03:03: 28000000 INFO @ Sat, 15 Jan 2022 19:03:04: 18000000 INFO @ Sat, 15 Jan 2022 19:03:09: 23000000 INFO @ Sat, 15 Jan 2022 19:03:10: 29000000 INFO @ Sat, 15 Jan 2022 19:03:11: 19000000 INFO @ Sat, 15 Jan 2022 19:03:15: 24000000 INFO @ Sat, 15 Jan 2022 19:03:16: 30000000 INFO @ Sat, 15 Jan 2022 19:03:17: 20000000 INFO @ Sat, 15 Jan 2022 19:03:22: 25000000 INFO @ Sat, 15 Jan 2022 19:03:22: 31000000 INFO @ Sat, 15 Jan 2022 19:03:24: 21000000 INFO @ Sat, 15 Jan 2022 19:03:28: 26000000 INFO @ Sat, 15 Jan 2022 19:03:29: 32000000 INFO @ Sat, 15 Jan 2022 19:03:30: 22000000 INFO @ Sat, 15 Jan 2022 19:03:35: 27000000 INFO @ Sat, 15 Jan 2022 19:03:35: 33000000 INFO @ Sat, 15 Jan 2022 19:03:37: 23000000 INFO @ Sat, 15 Jan 2022 19:03:42: 28000000 INFO @ Sat, 15 Jan 2022 19:03:42: 34000000 INFO @ Sat, 15 Jan 2022 19:03:43: 24000000 INFO @ Sat, 15 Jan 2022 19:03:48: 29000000 INFO @ Sat, 15 Jan 2022 19:03:48: 35000000 INFO @ Sat, 15 Jan 2022 19:03:49: 25000000 INFO @ Sat, 15 Jan 2022 19:03:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:03:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:03:50: #1 total tags in treatment: 17325996 INFO @ Sat, 15 Jan 2022 19:03:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:03:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:03:50: #1 tags after filtering in treatment: 10674909 INFO @ Sat, 15 Jan 2022 19:03:50: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:03:50: #1 finished! INFO @ Sat, 15 Jan 2022 19:03:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:03:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:03:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:03:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:03:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:03:54: 30000000 INFO @ Sat, 15 Jan 2022 19:03:56: 26000000 INFO @ Sat, 15 Jan 2022 19:04:01: 31000000 INFO @ Sat, 15 Jan 2022 19:04:02: 27000000 INFO @ Sat, 15 Jan 2022 19:04:07: 32000000 INFO @ Sat, 15 Jan 2022 19:04:09: 28000000 INFO @ Sat, 15 Jan 2022 19:04:14: 33000000 INFO @ Sat, 15 Jan 2022 19:04:15: 29000000 INFO @ Sat, 15 Jan 2022 19:04:20: 34000000 INFO @ Sat, 15 Jan 2022 19:04:22: 30000000 INFO @ Sat, 15 Jan 2022 19:04:27: 35000000 INFO @ Sat, 15 Jan 2022 19:04:28: 31000000 INFO @ Sat, 15 Jan 2022 19:04:28: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:04:28: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:04:28: #1 total tags in treatment: 17325996 INFO @ Sat, 15 Jan 2022 19:04:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:04:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:04:28: #1 tags after filtering in treatment: 10674909 INFO @ Sat, 15 Jan 2022 19:04:28: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:04:28: #1 finished! INFO @ Sat, 15 Jan 2022 19:04:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:04:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:04:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:04:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:04:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:04:34: 32000000 INFO @ Sat, 15 Jan 2022 19:04:40: 33000000 INFO @ Sat, 15 Jan 2022 19:04:46: 34000000 INFO @ Sat, 15 Jan 2022 19:04:51: 35000000 INFO @ Sat, 15 Jan 2022 19:04:53: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:04:53: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:04:53: #1 total tags in treatment: 17325996 INFO @ Sat, 15 Jan 2022 19:04:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:04:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:04:53: #1 tags after filtering in treatment: 10674909 INFO @ Sat, 15 Jan 2022 19:04:53: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:04:53: #1 finished! INFO @ Sat, 15 Jan 2022 19:04:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:04:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:04:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:04:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:04:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592381/SRX10592381.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling