Job ID = 14520179 SRX = SRX10592379 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:30:51 prefetch.2.10.7: 1) Downloading 'SRR14227563'... 2022-01-15T09:30:51 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:31:50 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:31:50 prefetch.2.10.7: 1) 'SRR14227563' was downloaded successfully 2022-01-15T09:31:50 prefetch.2.10.7: 'SRR14227563' has 0 unresolved dependencies Read 22492811 spots for SRR14227563/SRR14227563.sra Written 22492811 spots for SRR14227563/SRR14227563.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:59 22492811 reads; of these: 22492811 (100.00%) were paired; of these: 959880 (4.27%) aligned concordantly 0 times 17065051 (75.87%) aligned concordantly exactly 1 time 4467880 (19.86%) aligned concordantly >1 times ---- 959880 pairs aligned concordantly 0 times; of these: 52738 (5.49%) aligned discordantly 1 time ---- 907142 pairs aligned 0 times concordantly or discordantly; of these: 1814284 mates make up the pairs; of these: 1325420 (73.05%) aligned 0 times 371600 (20.48%) aligned exactly 1 time 117264 (6.46%) aligned >1 times 97.05% overall alignment rate Time searching: 00:13:59 Overall time: 00:13:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5476620 / 21580916 = 0.2538 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:12: 1000000 INFO @ Sat, 15 Jan 2022 18:57:17: 2000000 INFO @ Sat, 15 Jan 2022 18:57:22: 3000000 INFO @ Sat, 15 Jan 2022 18:57:28: 4000000 INFO @ Sat, 15 Jan 2022 18:57:33: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:38: 6000000 INFO @ Sat, 15 Jan 2022 18:57:44: 1000000 INFO @ Sat, 15 Jan 2022 18:57:45: 7000000 INFO @ Sat, 15 Jan 2022 18:57:50: 2000000 INFO @ Sat, 15 Jan 2022 18:57:51: 8000000 INFO @ Sat, 15 Jan 2022 18:57:57: 3000000 INFO @ Sat, 15 Jan 2022 18:57:58: 9000000 INFO @ Sat, 15 Jan 2022 18:58:04: 10000000 INFO @ Sat, 15 Jan 2022 18:58:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:58:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:58:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:58:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:58:11: 11000000 INFO @ Sat, 15 Jan 2022 18:58:11: 5000000 INFO @ Sat, 15 Jan 2022 18:58:14: 1000000 INFO @ Sat, 15 Jan 2022 18:58:17: 12000000 INFO @ Sat, 15 Jan 2022 18:58:18: 6000000 INFO @ Sat, 15 Jan 2022 18:58:22: 2000000 INFO @ Sat, 15 Jan 2022 18:58:24: 13000000 INFO @ Sat, 15 Jan 2022 18:58:25: 7000000 INFO @ Sat, 15 Jan 2022 18:58:29: 3000000 INFO @ Sat, 15 Jan 2022 18:58:31: 14000000 INFO @ Sat, 15 Jan 2022 18:58:33: 8000000 INFO @ Sat, 15 Jan 2022 18:58:37: 4000000 INFO @ Sat, 15 Jan 2022 18:58:37: 15000000 INFO @ Sat, 15 Jan 2022 18:58:40: 9000000 INFO @ Sat, 15 Jan 2022 18:58:44: 16000000 INFO @ Sat, 15 Jan 2022 18:58:45: 5000000 INFO @ Sat, 15 Jan 2022 18:58:47: 10000000 INFO @ Sat, 15 Jan 2022 18:58:51: 17000000 INFO @ Sat, 15 Jan 2022 18:58:52: 6000000 INFO @ Sat, 15 Jan 2022 18:58:54: 11000000 INFO @ Sat, 15 Jan 2022 18:58:57: 18000000 INFO @ Sat, 15 Jan 2022 18:59:00: 7000000 INFO @ Sat, 15 Jan 2022 18:59:01: 12000000 INFO @ Sat, 15 Jan 2022 18:59:04: 19000000 INFO @ Sat, 15 Jan 2022 18:59:07: 8000000 INFO @ Sat, 15 Jan 2022 18:59:08: 13000000 INFO @ Sat, 15 Jan 2022 18:59:10: 20000000 INFO @ Sat, 15 Jan 2022 18:59:15: 9000000 INFO @ Sat, 15 Jan 2022 18:59:16: 14000000 INFO @ Sat, 15 Jan 2022 18:59:17: 21000000 INFO @ Sat, 15 Jan 2022 18:59:23: 10000000 INFO @ Sat, 15 Jan 2022 18:59:23: 15000000 INFO @ Sat, 15 Jan 2022 18:59:23: 22000000 INFO @ Sat, 15 Jan 2022 18:59:30: 23000000 INFO @ Sat, 15 Jan 2022 18:59:30: 11000000 INFO @ Sat, 15 Jan 2022 18:59:31: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:59:37: 24000000 INFO @ Sat, 15 Jan 2022 18:59:38: 12000000 INFO @ Sat, 15 Jan 2022 18:59:38: 17000000 INFO @ Sat, 15 Jan 2022 18:59:43: 25000000 INFO @ Sat, 15 Jan 2022 18:59:45: 13000000 INFO @ Sat, 15 Jan 2022 18:59:46: 18000000 INFO @ Sat, 15 Jan 2022 18:59:50: 26000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:59:53: 14000000 INFO @ Sat, 15 Jan 2022 18:59:53: 19000000 INFO @ Sat, 15 Jan 2022 18:59:56: 27000000 INFO @ Sat, 15 Jan 2022 19:00:00: 20000000 INFO @ Sat, 15 Jan 2022 19:00:00: 15000000 INFO @ Sat, 15 Jan 2022 19:00:03: 28000000 INFO @ Sat, 15 Jan 2022 19:00:07: 21000000 INFO @ Sat, 15 Jan 2022 19:00:08: 16000000 INFO @ Sat, 15 Jan 2022 19:00:10: 29000000 INFO @ Sat, 15 Jan 2022 19:00:15: 22000000 INFO @ Sat, 15 Jan 2022 19:00:16: 17000000 INFO @ Sat, 15 Jan 2022 19:00:16: 30000000 INFO @ Sat, 15 Jan 2022 19:00:22: 23000000 INFO @ Sat, 15 Jan 2022 19:00:23: 31000000 INFO @ Sat, 15 Jan 2022 19:00:23: 18000000 INFO @ Sat, 15 Jan 2022 19:00:29: 32000000 INFO @ Sat, 15 Jan 2022 19:00:30: 24000000 INFO @ Sat, 15 Jan 2022 19:00:31: 19000000 INFO @ Sat, 15 Jan 2022 19:00:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:00:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:00:34: #1 total tags in treatment: 16067876 INFO @ Sat, 15 Jan 2022 19:00:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:00:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:00:34: #1 tags after filtering in treatment: 10192388 INFO @ Sat, 15 Jan 2022 19:00:34: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 19:00:34: #1 finished! INFO @ Sat, 15 Jan 2022 19:00:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:00:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:00:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:00:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:00:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:00:37: 25000000 INFO @ Sat, 15 Jan 2022 19:00:38: 20000000 INFO @ Sat, 15 Jan 2022 19:00:44: 26000000 INFO @ Sat, 15 Jan 2022 19:00:45: 21000000 INFO @ Sat, 15 Jan 2022 19:00:51: 27000000 INFO @ Sat, 15 Jan 2022 19:00:52: 22000000 INFO @ Sat, 15 Jan 2022 19:00:59: 28000000 INFO @ Sat, 15 Jan 2022 19:01:00: 23000000 INFO @ Sat, 15 Jan 2022 19:01:06: 29000000 INFO @ Sat, 15 Jan 2022 19:01:07: 24000000 INFO @ Sat, 15 Jan 2022 19:01:13: 30000000 INFO @ Sat, 15 Jan 2022 19:01:15: 25000000 INFO @ Sat, 15 Jan 2022 19:01:20: 31000000 INFO @ Sat, 15 Jan 2022 19:01:22: 26000000 INFO @ Sat, 15 Jan 2022 19:01:28: 32000000 INFO @ Sat, 15 Jan 2022 19:01:29: 27000000 INFO @ Sat, 15 Jan 2022 19:01:33: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:01:33: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:01:33: #1 total tags in treatment: 16067876 INFO @ Sat, 15 Jan 2022 19:01:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:01:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:01:33: #1 tags after filtering in treatment: 10192388 INFO @ Sat, 15 Jan 2022 19:01:33: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 19:01:33: #1 finished! INFO @ Sat, 15 Jan 2022 19:01:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:01:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:01:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:01:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:01:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:01:36: 28000000 INFO @ Sat, 15 Jan 2022 19:01:43: 29000000 INFO @ Sat, 15 Jan 2022 19:01:50: 30000000 INFO @ Sat, 15 Jan 2022 19:01:56: 31000000 INFO @ Sat, 15 Jan 2022 19:02:03: 32000000 INFO @ Sat, 15 Jan 2022 19:02:08: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:02:08: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:02:08: #1 total tags in treatment: 16067876 INFO @ Sat, 15 Jan 2022 19:02:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:02:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:02:08: #1 tags after filtering in treatment: 10192388 INFO @ Sat, 15 Jan 2022 19:02:08: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 19:02:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:02:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:02:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:02:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:02:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:02:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592379/SRX10592379.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling