Job ID = 14520178 SRX = SRX10592378 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:30:36 prefetch.2.10.7: 1) Downloading 'SRR14227562'... 2022-01-15T09:30:36 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:31:28 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:31:28 prefetch.2.10.7: 1) 'SRR14227562' was downloaded successfully 2022-01-15T09:31:28 prefetch.2.10.7: 'SRR14227562' has 0 unresolved dependencies Read 22995455 spots for SRR14227562/SRR14227562.sra Written 22995455 spots for SRR14227562/SRR14227562.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:10 22995455 reads; of these: 22995455 (100.00%) were paired; of these: 1104769 (4.80%) aligned concordantly 0 times 17389992 (75.62%) aligned concordantly exactly 1 time 4500694 (19.57%) aligned concordantly >1 times ---- 1104769 pairs aligned concordantly 0 times; of these: 45516 (4.12%) aligned discordantly 1 time ---- 1059253 pairs aligned 0 times concordantly or discordantly; of these: 2118506 mates make up the pairs; of these: 1624698 (76.69%) aligned 0 times 392140 (18.51%) aligned exactly 1 time 101668 (4.80%) aligned >1 times 96.47% overall alignment rate Time searching: 00:13:10 Overall time: 00:13:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 6165780 / 21931990 = 0.2811 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:54:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:54:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:54:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:54:58: 1000000 INFO @ Sat, 15 Jan 2022 18:55:03: 2000000 INFO @ Sat, 15 Jan 2022 18:55:08: 3000000 INFO @ Sat, 15 Jan 2022 18:55:13: 4000000 INFO @ Sat, 15 Jan 2022 18:55:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:55:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:55:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:55:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:55:24: 6000000 INFO @ Sat, 15 Jan 2022 18:55:28: 1000000 INFO @ Sat, 15 Jan 2022 18:55:29: 7000000 INFO @ Sat, 15 Jan 2022 18:55:34: 2000000 INFO @ Sat, 15 Jan 2022 18:55:34: 8000000 INFO @ Sat, 15 Jan 2022 18:55:39: 3000000 INFO @ Sat, 15 Jan 2022 18:55:40: 9000000 INFO @ Sat, 15 Jan 2022 18:55:44: 4000000 INFO @ Sat, 15 Jan 2022 18:55:45: 10000000 INFO @ Sat, 15 Jan 2022 18:55:50: 5000000 INFO @ Sat, 15 Jan 2022 18:55:51: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:55:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:55:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:55:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:55:55: 6000000 INFO @ Sat, 15 Jan 2022 18:55:56: 12000000 INFO @ Sat, 15 Jan 2022 18:55:58: 1000000 INFO @ Sat, 15 Jan 2022 18:56:00: 7000000 INFO @ Sat, 15 Jan 2022 18:56:02: 13000000 INFO @ Sat, 15 Jan 2022 18:56:04: 2000000 INFO @ Sat, 15 Jan 2022 18:56:06: 8000000 INFO @ Sat, 15 Jan 2022 18:56:07: 14000000 INFO @ Sat, 15 Jan 2022 18:56:09: 3000000 INFO @ Sat, 15 Jan 2022 18:56:11: 9000000 INFO @ Sat, 15 Jan 2022 18:56:13: 15000000 INFO @ Sat, 15 Jan 2022 18:56:15: 4000000 INFO @ Sat, 15 Jan 2022 18:56:16: 10000000 INFO @ Sat, 15 Jan 2022 18:56:18: 16000000 INFO @ Sat, 15 Jan 2022 18:56:21: 5000000 INFO @ Sat, 15 Jan 2022 18:56:22: 11000000 INFO @ Sat, 15 Jan 2022 18:56:24: 17000000 INFO @ Sat, 15 Jan 2022 18:56:26: 6000000 INFO @ Sat, 15 Jan 2022 18:56:27: 12000000 INFO @ Sat, 15 Jan 2022 18:56:29: 18000000 INFO @ Sat, 15 Jan 2022 18:56:32: 7000000 INFO @ Sat, 15 Jan 2022 18:56:33: 13000000 INFO @ Sat, 15 Jan 2022 18:56:34: 19000000 INFO @ Sat, 15 Jan 2022 18:56:37: 8000000 INFO @ Sat, 15 Jan 2022 18:56:39: 14000000 INFO @ Sat, 15 Jan 2022 18:56:40: 20000000 INFO @ Sat, 15 Jan 2022 18:56:43: 9000000 INFO @ Sat, 15 Jan 2022 18:56:44: 15000000 INFO @ Sat, 15 Jan 2022 18:56:45: 21000000 INFO @ Sat, 15 Jan 2022 18:56:49: 10000000 INFO @ Sat, 15 Jan 2022 18:56:50: 16000000 INFO @ Sat, 15 Jan 2022 18:56:50: 22000000 INFO @ Sat, 15 Jan 2022 18:56:55: 11000000 INFO @ Sat, 15 Jan 2022 18:56:55: 17000000 INFO @ Sat, 15 Jan 2022 18:56:56: 23000000 INFO @ Sat, 15 Jan 2022 18:57:00: 12000000 INFO @ Sat, 15 Jan 2022 18:57:01: 18000000 INFO @ Sat, 15 Jan 2022 18:57:01: 24000000 INFO @ Sat, 15 Jan 2022 18:57:06: 13000000 INFO @ Sat, 15 Jan 2022 18:57:07: 19000000 INFO @ Sat, 15 Jan 2022 18:57:07: 25000000 INFO @ Sat, 15 Jan 2022 18:57:12: 14000000 INFO @ Sat, 15 Jan 2022 18:57:12: 20000000 INFO @ Sat, 15 Jan 2022 18:57:13: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:57:18: 15000000 INFO @ Sat, 15 Jan 2022 18:57:18: 21000000 INFO @ Sat, 15 Jan 2022 18:57:19: 27000000 INFO @ Sat, 15 Jan 2022 18:57:23: 22000000 INFO @ Sat, 15 Jan 2022 18:57:24: 16000000 INFO @ Sat, 15 Jan 2022 18:57:25: 28000000 INFO @ Sat, 15 Jan 2022 18:57:29: 23000000 INFO @ Sat, 15 Jan 2022 18:57:29: 17000000 INFO @ Sat, 15 Jan 2022 18:57:31: 29000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:57:34: 24000000 INFO @ Sat, 15 Jan 2022 18:57:35: 18000000 INFO @ Sat, 15 Jan 2022 18:57:36: 30000000 INFO @ Sat, 15 Jan 2022 18:57:40: 25000000 INFO @ Sat, 15 Jan 2022 18:57:41: 19000000 INFO @ Sat, 15 Jan 2022 18:57:42: 31000000 INFO @ Sat, 15 Jan 2022 18:57:45: 26000000 INFO @ Sat, 15 Jan 2022 18:57:47: 20000000 INFO @ Sat, 15 Jan 2022 18:57:48: 32000000 INFO @ Sat, 15 Jan 2022 18:57:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:57:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:57:48: #1 total tags in treatment: 15735850 INFO @ Sat, 15 Jan 2022 18:57:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:57:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:57:49: #1 tags after filtering in treatment: 10216607 INFO @ Sat, 15 Jan 2022 18:57:49: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:57:49: #1 finished! INFO @ Sat, 15 Jan 2022 18:57:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:57:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:57:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:57:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:57:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:57:51: 27000000 INFO @ Sat, 15 Jan 2022 18:57:52: 21000000 INFO @ Sat, 15 Jan 2022 18:57:57: 28000000 INFO @ Sat, 15 Jan 2022 18:57:58: 22000000 INFO @ Sat, 15 Jan 2022 18:58:02: 29000000 INFO @ Sat, 15 Jan 2022 18:58:03: 23000000 INFO @ Sat, 15 Jan 2022 18:58:08: 30000000 INFO @ Sat, 15 Jan 2022 18:58:09: 24000000 INFO @ Sat, 15 Jan 2022 18:58:13: 31000000 INFO @ Sat, 15 Jan 2022 18:58:14: 25000000 INFO @ Sat, 15 Jan 2022 18:58:19: 32000000 INFO @ Sat, 15 Jan 2022 18:58:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:19: #1 total tags in treatment: 15735850 INFO @ Sat, 15 Jan 2022 18:58:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:19: #1 tags after filtering in treatment: 10216607 INFO @ Sat, 15 Jan 2022 18:58:19: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:58:19: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:20: 26000000 INFO @ Sat, 15 Jan 2022 18:58:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:58:25: 27000000 INFO @ Sat, 15 Jan 2022 18:58:30: 28000000 INFO @ Sat, 15 Jan 2022 18:58:36: 29000000 INFO @ Sat, 15 Jan 2022 18:58:41: 30000000 INFO @ Sat, 15 Jan 2022 18:58:46: 31000000 INFO @ Sat, 15 Jan 2022 18:58:51: 32000000 INFO @ Sat, 15 Jan 2022 18:58:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:52: #1 total tags in treatment: 15735850 INFO @ Sat, 15 Jan 2022 18:58:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:52: #1 tags after filtering in treatment: 10216607 INFO @ Sat, 15 Jan 2022 18:58:52: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:58:52: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592378/SRX10592378.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling