Job ID = 14520176 SRX = SRX10592376 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:30:21 prefetch.2.10.7: 1) Downloading 'SRR14227560'... 2022-01-15T09:30:21 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:31:27 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:31:27 prefetch.2.10.7: 1) 'SRR14227560' was downloaded successfully 2022-01-15T09:31:27 prefetch.2.10.7: 'SRR14227560' has 0 unresolved dependencies Read 23837397 spots for SRR14227560/SRR14227560.sra Written 23837397 spots for SRR14227560/SRR14227560.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:34 23837397 reads; of these: 23837397 (100.00%) were paired; of these: 855431 (3.59%) aligned concordantly 0 times 17982169 (75.44%) aligned concordantly exactly 1 time 4999797 (20.97%) aligned concordantly >1 times ---- 855431 pairs aligned concordantly 0 times; of these: 45902 (5.37%) aligned discordantly 1 time ---- 809529 pairs aligned 0 times concordantly or discordantly; of these: 1619058 mates make up the pairs; of these: 1207630 (74.59%) aligned 0 times 294990 (18.22%) aligned exactly 1 time 116438 (7.19%) aligned >1 times 97.47% overall alignment rate Time searching: 00:14:34 Overall time: 00:14:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 7395586 / 23022838 = 0.3212 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:06: 1000000 INFO @ Sat, 15 Jan 2022 18:57:10: 2000000 INFO @ Sat, 15 Jan 2022 18:57:15: 3000000 INFO @ Sat, 15 Jan 2022 18:57:19: 4000000 INFO @ Sat, 15 Jan 2022 18:57:24: 5000000 INFO @ Sat, 15 Jan 2022 18:57:28: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:33: 7000000 INFO @ Sat, 15 Jan 2022 18:57:36: 1000000 INFO @ Sat, 15 Jan 2022 18:57:38: 8000000 INFO @ Sat, 15 Jan 2022 18:57:41: 2000000 INFO @ Sat, 15 Jan 2022 18:57:43: 9000000 INFO @ Sat, 15 Jan 2022 18:57:46: 3000000 INFO @ Sat, 15 Jan 2022 18:57:48: 10000000 INFO @ Sat, 15 Jan 2022 18:57:50: 4000000 INFO @ Sat, 15 Jan 2022 18:57:52: 11000000 INFO @ Sat, 15 Jan 2022 18:57:55: 5000000 INFO @ Sat, 15 Jan 2022 18:57:57: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:58:00: 6000000 INFO @ Sat, 15 Jan 2022 18:58:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:58:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:58:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:58:02: 13000000 INFO @ Sat, 15 Jan 2022 18:58:05: 7000000 INFO @ Sat, 15 Jan 2022 18:58:07: 1000000 INFO @ Sat, 15 Jan 2022 18:58:07: 14000000 INFO @ Sat, 15 Jan 2022 18:58:10: 8000000 INFO @ Sat, 15 Jan 2022 18:58:12: 15000000 INFO @ Sat, 15 Jan 2022 18:58:13: 2000000 INFO @ Sat, 15 Jan 2022 18:58:16: 9000000 INFO @ Sat, 15 Jan 2022 18:58:18: 16000000 INFO @ Sat, 15 Jan 2022 18:58:19: 3000000 INFO @ Sat, 15 Jan 2022 18:58:21: 10000000 INFO @ Sat, 15 Jan 2022 18:58:23: 17000000 INFO @ Sat, 15 Jan 2022 18:58:24: 4000000 INFO @ Sat, 15 Jan 2022 18:58:26: 11000000 INFO @ Sat, 15 Jan 2022 18:58:28: 18000000 INFO @ Sat, 15 Jan 2022 18:58:30: 5000000 INFO @ Sat, 15 Jan 2022 18:58:31: 12000000 INFO @ Sat, 15 Jan 2022 18:58:33: 19000000 INFO @ Sat, 15 Jan 2022 18:58:35: 6000000 INFO @ Sat, 15 Jan 2022 18:58:36: 13000000 INFO @ Sat, 15 Jan 2022 18:58:38: 20000000 INFO @ Sat, 15 Jan 2022 18:58:41: 14000000 INFO @ Sat, 15 Jan 2022 18:58:41: 7000000 INFO @ Sat, 15 Jan 2022 18:58:43: 21000000 INFO @ Sat, 15 Jan 2022 18:58:46: 15000000 INFO @ Sat, 15 Jan 2022 18:58:47: 8000000 INFO @ Sat, 15 Jan 2022 18:58:48: 22000000 INFO @ Sat, 15 Jan 2022 18:58:51: 16000000 INFO @ Sat, 15 Jan 2022 18:58:52: 9000000 INFO @ Sat, 15 Jan 2022 18:58:53: 23000000 INFO @ Sat, 15 Jan 2022 18:58:56: 17000000 INFO @ Sat, 15 Jan 2022 18:58:57: 24000000 INFO @ Sat, 15 Jan 2022 18:58:58: 10000000 INFO @ Sat, 15 Jan 2022 18:59:01: 18000000 INFO @ Sat, 15 Jan 2022 18:59:02: 25000000 INFO @ Sat, 15 Jan 2022 18:59:03: 11000000 INFO @ Sat, 15 Jan 2022 18:59:06: 19000000 INFO @ Sat, 15 Jan 2022 18:59:07: 26000000 INFO @ Sat, 15 Jan 2022 18:59:09: 12000000 INFO @ Sat, 15 Jan 2022 18:59:11: 20000000 INFO @ Sat, 15 Jan 2022 18:59:12: 27000000 INFO @ Sat, 15 Jan 2022 18:59:14: 13000000 INFO @ Sat, 15 Jan 2022 18:59:16: 21000000 INFO @ Sat, 15 Jan 2022 18:59:18: 28000000 INFO @ Sat, 15 Jan 2022 18:59:20: 14000000 INFO @ Sat, 15 Jan 2022 18:59:21: 22000000 INFO @ Sat, 15 Jan 2022 18:59:23: 29000000 INFO @ Sat, 15 Jan 2022 18:59:26: 15000000 INFO @ Sat, 15 Jan 2022 18:59:26: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:59:28: 30000000 INFO @ Sat, 15 Jan 2022 18:59:31: 24000000 INFO @ Sat, 15 Jan 2022 18:59:31: 16000000 INFO @ Sat, 15 Jan 2022 18:59:33: 31000000 INFO @ Sat, 15 Jan 2022 18:59:36: 25000000 INFO @ Sat, 15 Jan 2022 18:59:36: 17000000 INFO @ Sat, 15 Jan 2022 18:59:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:59:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:59:36: #1 total tags in treatment: 15599352 INFO @ Sat, 15 Jan 2022 18:59:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:37: #1 tags after filtering in treatment: 9796374 INFO @ Sat, 15 Jan 2022 18:59:37: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 18:59:37: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:59:41: 26000000 INFO @ Sat, 15 Jan 2022 18:59:42: 18000000 INFO @ Sat, 15 Jan 2022 18:59:46: 27000000 INFO @ Sat, 15 Jan 2022 18:59:47: 19000000 INFO @ Sat, 15 Jan 2022 18:59:51: 28000000 INFO @ Sat, 15 Jan 2022 18:59:53: 20000000 INFO @ Sat, 15 Jan 2022 18:59:56: 29000000 INFO @ Sat, 15 Jan 2022 18:59:58: 21000000 INFO @ Sat, 15 Jan 2022 19:00:01: 30000000 INFO @ Sat, 15 Jan 2022 19:00:04: 22000000 INFO @ Sat, 15 Jan 2022 19:00:06: 31000000 INFO @ Sat, 15 Jan 2022 19:00:09: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:00:09: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:00:09: #1 total tags in treatment: 15599352 INFO @ Sat, 15 Jan 2022 19:00:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:00:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:00:10: #1 tags after filtering in treatment: 9796374 INFO @ Sat, 15 Jan 2022 19:00:10: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 19:00:10: #1 finished! INFO @ Sat, 15 Jan 2022 19:00:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:00:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:00:10: 23000000 INFO @ Sat, 15 Jan 2022 19:00:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:00:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:00:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:00:15: 24000000 INFO @ Sat, 15 Jan 2022 19:00:20: 25000000 INFO @ Sat, 15 Jan 2022 19:00:26: 26000000 INFO @ Sat, 15 Jan 2022 19:00:31: 27000000 INFO @ Sat, 15 Jan 2022 19:00:36: 28000000 INFO @ Sat, 15 Jan 2022 19:00:41: 29000000 INFO @ Sat, 15 Jan 2022 19:00:47: 30000000 INFO @ Sat, 15 Jan 2022 19:00:52: 31000000 INFO @ Sat, 15 Jan 2022 19:00:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:00:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:00:56: #1 total tags in treatment: 15599352 INFO @ Sat, 15 Jan 2022 19:00:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:00:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:00:56: #1 tags after filtering in treatment: 9796374 INFO @ Sat, 15 Jan 2022 19:00:56: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 19:00:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:00:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:00:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:00:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:00:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:00:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592376/SRX10592376.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling