Job ID = 14520175 SRX = SRX10592375 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:30:21 prefetch.2.10.7: 1) Downloading 'SRR14227559'... 2022-01-15T09:30:21 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:31:25 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:31:25 prefetch.2.10.7: 1) 'SRR14227559' was downloaded successfully 2022-01-15T09:31:25 prefetch.2.10.7: 'SRR14227559' has 0 unresolved dependencies Read 23508903 spots for SRR14227559/SRR14227559.sra Written 23508903 spots for SRR14227559/SRR14227559.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:39 23508903 reads; of these: 23508903 (100.00%) were paired; of these: 903686 (3.84%) aligned concordantly 0 times 17701583 (75.30%) aligned concordantly exactly 1 time 4903634 (20.86%) aligned concordantly >1 times ---- 903686 pairs aligned concordantly 0 times; of these: 54150 (5.99%) aligned discordantly 1 time ---- 849536 pairs aligned 0 times concordantly or discordantly; of these: 1699072 mates make up the pairs; of these: 1251218 (73.64%) aligned 0 times 319932 (18.83%) aligned exactly 1 time 127922 (7.53%) aligned >1 times 97.34% overall alignment rate Time searching: 00:13:39 Overall time: 00:13:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 6372806 / 22653880 = 0.2813 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:55:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:55:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:55:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:55:37: 1000000 INFO @ Sat, 15 Jan 2022 18:55:41: 2000000 INFO @ Sat, 15 Jan 2022 18:55:45: 3000000 INFO @ Sat, 15 Jan 2022 18:55:49: 4000000 INFO @ Sat, 15 Jan 2022 18:55:54: 5000000 INFO @ Sat, 15 Jan 2022 18:55:58: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:02: 7000000 INFO @ Sat, 15 Jan 2022 18:56:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:06: 8000000 INFO @ Sat, 15 Jan 2022 18:56:08: 1000000 INFO @ Sat, 15 Jan 2022 18:56:11: 9000000 INFO @ Sat, 15 Jan 2022 18:56:13: 2000000 INFO @ Sat, 15 Jan 2022 18:56:15: 10000000 INFO @ Sat, 15 Jan 2022 18:56:18: 3000000 INFO @ Sat, 15 Jan 2022 18:56:20: 11000000 INFO @ Sat, 15 Jan 2022 18:56:23: 4000000 INFO @ Sat, 15 Jan 2022 18:56:24: 12000000 INFO @ Sat, 15 Jan 2022 18:56:28: 5000000 INFO @ Sat, 15 Jan 2022 18:56:29: 13000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:33: 14000000 INFO @ Sat, 15 Jan 2022 18:56:33: 6000000 INFO @ Sat, 15 Jan 2022 18:56:37: 1000000 INFO @ Sat, 15 Jan 2022 18:56:38: 15000000 INFO @ Sat, 15 Jan 2022 18:56:39: 7000000 INFO @ Sat, 15 Jan 2022 18:56:42: 2000000 INFO @ Sat, 15 Jan 2022 18:56:43: 16000000 INFO @ Sat, 15 Jan 2022 18:56:44: 8000000 INFO @ Sat, 15 Jan 2022 18:56:47: 3000000 INFO @ Sat, 15 Jan 2022 18:56:47: 17000000 INFO @ Sat, 15 Jan 2022 18:56:49: 9000000 INFO @ Sat, 15 Jan 2022 18:56:51: 4000000 INFO @ Sat, 15 Jan 2022 18:56:52: 18000000 INFO @ Sat, 15 Jan 2022 18:56:54: 10000000 INFO @ Sat, 15 Jan 2022 18:56:56: 5000000 INFO @ Sat, 15 Jan 2022 18:56:57: 19000000 INFO @ Sat, 15 Jan 2022 18:56:59: 11000000 INFO @ Sat, 15 Jan 2022 18:57:00: 6000000 INFO @ Sat, 15 Jan 2022 18:57:01: 20000000 INFO @ Sat, 15 Jan 2022 18:57:05: 12000000 INFO @ Sat, 15 Jan 2022 18:57:05: 7000000 INFO @ Sat, 15 Jan 2022 18:57:06: 21000000 INFO @ Sat, 15 Jan 2022 18:57:09: 8000000 INFO @ Sat, 15 Jan 2022 18:57:10: 13000000 INFO @ Sat, 15 Jan 2022 18:57:10: 22000000 INFO @ Sat, 15 Jan 2022 18:57:14: 9000000 INFO @ Sat, 15 Jan 2022 18:57:15: 23000000 INFO @ Sat, 15 Jan 2022 18:57:15: 14000000 INFO @ Sat, 15 Jan 2022 18:57:19: 10000000 INFO @ Sat, 15 Jan 2022 18:57:19: 24000000 INFO @ Sat, 15 Jan 2022 18:57:20: 15000000 INFO @ Sat, 15 Jan 2022 18:57:23: 11000000 INFO @ Sat, 15 Jan 2022 18:57:24: 25000000 INFO @ Sat, 15 Jan 2022 18:57:25: 16000000 INFO @ Sat, 15 Jan 2022 18:57:28: 12000000 INFO @ Sat, 15 Jan 2022 18:57:29: 26000000 INFO @ Sat, 15 Jan 2022 18:57:30: 17000000 INFO @ Sat, 15 Jan 2022 18:57:33: 13000000 INFO @ Sat, 15 Jan 2022 18:57:33: 27000000 INFO @ Sat, 15 Jan 2022 18:57:35: 18000000 INFO @ Sat, 15 Jan 2022 18:57:37: 14000000 INFO @ Sat, 15 Jan 2022 18:57:38: 28000000 INFO @ Sat, 15 Jan 2022 18:57:41: 19000000 INFO @ Sat, 15 Jan 2022 18:57:42: 15000000 INFO @ Sat, 15 Jan 2022 18:57:43: 29000000 INFO @ Sat, 15 Jan 2022 18:57:46: 20000000 INFO @ Sat, 15 Jan 2022 18:57:46: 16000000 INFO @ Sat, 15 Jan 2022 18:57:47: 30000000 INFO @ Sat, 15 Jan 2022 18:57:51: 21000000 INFO @ Sat, 15 Jan 2022 18:57:51: 17000000 INFO @ Sat, 15 Jan 2022 18:57:52: 31000000 INFO @ Sat, 15 Jan 2022 18:57:56: 18000000 INFO @ Sat, 15 Jan 2022 18:57:56: 22000000 INFO @ Sat, 15 Jan 2022 18:57:56: 32000000 INFO @ Sat, 15 Jan 2022 18:58:00: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:58:01: 23000000 INFO @ Sat, 15 Jan 2022 18:58:01: 33000000 INFO @ Sat, 15 Jan 2022 18:58:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:01: #1 total tags in treatment: 16245586 INFO @ Sat, 15 Jan 2022 18:58:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:02: #1 tags after filtering in treatment: 10054929 INFO @ Sat, 15 Jan 2022 18:58:02: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 18:58:02: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:58:05: 20000000 INFO @ Sat, 15 Jan 2022 18:58:06: 24000000 INFO @ Sat, 15 Jan 2022 18:58:09: 21000000 INFO @ Sat, 15 Jan 2022 18:58:11: 25000000 INFO @ Sat, 15 Jan 2022 18:58:13: 22000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:58:16: 26000000 INFO @ Sat, 15 Jan 2022 18:58:18: 23000000 INFO @ Sat, 15 Jan 2022 18:58:21: 27000000 INFO @ Sat, 15 Jan 2022 18:58:22: 24000000 INFO @ Sat, 15 Jan 2022 18:58:27: 28000000 INFO @ Sat, 15 Jan 2022 18:58:27: 25000000 INFO @ Sat, 15 Jan 2022 18:58:31: 26000000 INFO @ Sat, 15 Jan 2022 18:58:32: 29000000 INFO @ Sat, 15 Jan 2022 18:58:36: 27000000 INFO @ Sat, 15 Jan 2022 18:58:37: 30000000 INFO @ Sat, 15 Jan 2022 18:58:40: 28000000 INFO @ Sat, 15 Jan 2022 18:58:42: 31000000 INFO @ Sat, 15 Jan 2022 18:58:45: 29000000 INFO @ Sat, 15 Jan 2022 18:58:47: 32000000 INFO @ Sat, 15 Jan 2022 18:58:49: 30000000 INFO @ Sat, 15 Jan 2022 18:58:52: 33000000 INFO @ Sat, 15 Jan 2022 18:58:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:52: #1 total tags in treatment: 16245586 INFO @ Sat, 15 Jan 2022 18:58:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:52: #1 tags after filtering in treatment: 10054929 INFO @ Sat, 15 Jan 2022 18:58:52: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 18:58:52: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:58:54: 31000000 INFO @ Sat, 15 Jan 2022 18:58:58: 32000000 INFO @ Sat, 15 Jan 2022 18:59:02: 33000000 INFO @ Sat, 15 Jan 2022 18:59:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:59:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:59:03: #1 total tags in treatment: 16245586 INFO @ Sat, 15 Jan 2022 18:59:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:03: #1 tags after filtering in treatment: 10054929 INFO @ Sat, 15 Jan 2022 18:59:03: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 18:59:03: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592375/SRX10592375.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling