Job ID = 14520174 SRX = SRX10592374 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:30:06 prefetch.2.10.7: 1) Downloading 'SRR14227558'... 2022-01-15T09:30:06 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:31:07 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:31:07 prefetch.2.10.7: 1) 'SRR14227558' was downloaded successfully 2022-01-15T09:31:07 prefetch.2.10.7: 'SRR14227558' has 0 unresolved dependencies Read 26099085 spots for SRR14227558/SRR14227558.sra Written 26099085 spots for SRR14227558/SRR14227558.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:57 26099085 reads; of these: 26099085 (100.00%) were paired; of these: 1488745 (5.70%) aligned concordantly 0 times 18630529 (71.38%) aligned concordantly exactly 1 time 5979811 (22.91%) aligned concordantly >1 times ---- 1488745 pairs aligned concordantly 0 times; of these: 81047 (5.44%) aligned discordantly 1 time ---- 1407698 pairs aligned 0 times concordantly or discordantly; of these: 2815396 mates make up the pairs; of these: 2165742 (76.92%) aligned 0 times 500669 (17.78%) aligned exactly 1 time 148985 (5.29%) aligned >1 times 95.85% overall alignment rate Time searching: 00:14:57 Overall time: 00:14:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 12682124 / 24686591 = 0.5137 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:59: 1000000 INFO @ Sat, 15 Jan 2022 18:57:03: 2000000 INFO @ Sat, 15 Jan 2022 18:57:07: 3000000 INFO @ Sat, 15 Jan 2022 18:57:11: 4000000 INFO @ Sat, 15 Jan 2022 18:57:15: 5000000 INFO @ Sat, 15 Jan 2022 18:57:19: 6000000 INFO @ Sat, 15 Jan 2022 18:57:23: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:27: 8000000 INFO @ Sat, 15 Jan 2022 18:57:29: 1000000 INFO @ Sat, 15 Jan 2022 18:57:31: 9000000 INFO @ Sat, 15 Jan 2022 18:57:33: 2000000 INFO @ Sat, 15 Jan 2022 18:57:35: 10000000 INFO @ Sat, 15 Jan 2022 18:57:37: 3000000 INFO @ Sat, 15 Jan 2022 18:57:39: 11000000 INFO @ Sat, 15 Jan 2022 18:57:41: 4000000 INFO @ Sat, 15 Jan 2022 18:57:44: 12000000 INFO @ Sat, 15 Jan 2022 18:57:46: 5000000 INFO @ Sat, 15 Jan 2022 18:57:48: 13000000 INFO @ Sat, 15 Jan 2022 18:57:50: 6000000 INFO @ Sat, 15 Jan 2022 18:57:52: 14000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:54: 7000000 INFO @ Sat, 15 Jan 2022 18:57:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:56: 15000000 INFO @ Sat, 15 Jan 2022 18:57:58: 8000000 INFO @ Sat, 15 Jan 2022 18:57:59: 1000000 INFO @ Sat, 15 Jan 2022 18:58:00: 16000000 INFO @ Sat, 15 Jan 2022 18:58:02: 9000000 INFO @ Sat, 15 Jan 2022 18:58:04: 2000000 INFO @ Sat, 15 Jan 2022 18:58:04: 17000000 INFO @ Sat, 15 Jan 2022 18:58:07: 10000000 INFO @ Sat, 15 Jan 2022 18:58:08: 3000000 INFO @ Sat, 15 Jan 2022 18:58:08: 18000000 INFO @ Sat, 15 Jan 2022 18:58:11: 11000000 INFO @ Sat, 15 Jan 2022 18:58:12: 19000000 INFO @ Sat, 15 Jan 2022 18:58:12: 4000000 INFO @ Sat, 15 Jan 2022 18:58:15: 12000000 INFO @ Sat, 15 Jan 2022 18:58:16: 20000000 INFO @ Sat, 15 Jan 2022 18:58:16: 5000000 INFO @ Sat, 15 Jan 2022 18:58:19: 13000000 INFO @ Sat, 15 Jan 2022 18:58:20: 21000000 INFO @ Sat, 15 Jan 2022 18:58:21: 6000000 INFO @ Sat, 15 Jan 2022 18:58:23: 14000000 INFO @ Sat, 15 Jan 2022 18:58:24: 22000000 INFO @ Sat, 15 Jan 2022 18:58:25: 7000000 INFO @ Sat, 15 Jan 2022 18:58:28: 15000000 INFO @ Sat, 15 Jan 2022 18:58:29: 23000000 INFO @ Sat, 15 Jan 2022 18:58:29: 8000000 INFO @ Sat, 15 Jan 2022 18:58:32: 16000000 INFO @ Sat, 15 Jan 2022 18:58:33: 24000000 INFO @ Sat, 15 Jan 2022 18:58:33: 9000000 INFO @ Sat, 15 Jan 2022 18:58:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:36: #1 total tags in treatment: 11965996 INFO @ Sat, 15 Jan 2022 18:58:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:36: 17000000 INFO @ Sat, 15 Jan 2022 18:58:36: #1 tags after filtering in treatment: 7874744 INFO @ Sat, 15 Jan 2022 18:58:36: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 18:58:36: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:58:38: 10000000 INFO @ Sat, 15 Jan 2022 18:58:40: 18000000 INFO @ Sat, 15 Jan 2022 18:58:42: 11000000 INFO @ Sat, 15 Jan 2022 18:58:44: 19000000 INFO @ Sat, 15 Jan 2022 18:58:46: 12000000 INFO @ Sat, 15 Jan 2022 18:58:48: 20000000 INFO @ Sat, 15 Jan 2022 18:58:50: 13000000 INFO @ Sat, 15 Jan 2022 18:58:53: 21000000 INFO @ Sat, 15 Jan 2022 18:58:54: 14000000 INFO @ Sat, 15 Jan 2022 18:58:57: 22000000 INFO @ Sat, 15 Jan 2022 18:58:59: 15000000 INFO @ Sat, 15 Jan 2022 18:59:01: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:59:03: 16000000 INFO @ Sat, 15 Jan 2022 18:59:05: 24000000 INFO @ Sat, 15 Jan 2022 18:59:07: 17000000 INFO @ Sat, 15 Jan 2022 18:59:08: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:59:08: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:59:08: #1 total tags in treatment: 11965996 INFO @ Sat, 15 Jan 2022 18:59:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:08: #1 tags after filtering in treatment: 7874744 INFO @ Sat, 15 Jan 2022 18:59:08: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 18:59:08: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:59:11: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:59:15: 19000000 INFO @ Sat, 15 Jan 2022 18:59:19: 20000000 INFO @ Sat, 15 Jan 2022 18:59:23: 21000000 INFO @ Sat, 15 Jan 2022 18:59:27: 22000000 INFO @ Sat, 15 Jan 2022 18:59:31: 23000000 INFO @ Sat, 15 Jan 2022 18:59:36: 24000000 INFO @ Sat, 15 Jan 2022 18:59:38: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:59:38: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:59:38: #1 total tags in treatment: 11965996 INFO @ Sat, 15 Jan 2022 18:59:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:39: #1 tags after filtering in treatment: 7874744 INFO @ Sat, 15 Jan 2022 18:59:39: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 18:59:39: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592374/SRX10592374.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling