Job ID = 14520172 SRX = SRX10592373 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:30:06 prefetch.2.10.7: 1) Downloading 'SRR14227557'... 2022-01-15T09:30:06 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:31:16 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:31:16 prefetch.2.10.7: 1) 'SRR14227557' was downloaded successfully 2022-01-15T09:31:16 prefetch.2.10.7: 'SRR14227557' has 0 unresolved dependencies Read 25311223 spots for SRR14227557/SRR14227557.sra Written 25311223 spots for SRR14227557/SRR14227557.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:19 25311223 reads; of these: 25311223 (100.00%) were paired; of these: 1995419 (7.88%) aligned concordantly 0 times 18157864 (71.74%) aligned concordantly exactly 1 time 5157940 (20.38%) aligned concordantly >1 times ---- 1995419 pairs aligned concordantly 0 times; of these: 175029 (8.77%) aligned discordantly 1 time ---- 1820390 pairs aligned 0 times concordantly or discordantly; of these: 3640780 mates make up the pairs; of these: 2885247 (79.25%) aligned 0 times 571629 (15.70%) aligned exactly 1 time 183904 (5.05%) aligned >1 times 94.30% overall alignment rate Time searching: 00:14:19 Overall time: 00:14:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 15483798 / 23485749 = 0.6593 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:55:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:55:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:55:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:55:43: 1000000 INFO @ Sat, 15 Jan 2022 18:55:47: 2000000 INFO @ Sat, 15 Jan 2022 18:55:51: 3000000 INFO @ Sat, 15 Jan 2022 18:55:56: 4000000 INFO @ Sat, 15 Jan 2022 18:56:00: 5000000 INFO @ Sat, 15 Jan 2022 18:56:04: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:08: 7000000 INFO @ Sat, 15 Jan 2022 18:56:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:13: 8000000 INFO @ Sat, 15 Jan 2022 18:56:13: 1000000 INFO @ Sat, 15 Jan 2022 18:56:17: 9000000 INFO @ Sat, 15 Jan 2022 18:56:18: 2000000 INFO @ Sat, 15 Jan 2022 18:56:21: 10000000 INFO @ Sat, 15 Jan 2022 18:56:22: 3000000 INFO @ Sat, 15 Jan 2022 18:56:25: 11000000 INFO @ Sat, 15 Jan 2022 18:56:26: 4000000 INFO @ Sat, 15 Jan 2022 18:56:30: 12000000 INFO @ Sat, 15 Jan 2022 18:56:31: 5000000 INFO @ Sat, 15 Jan 2022 18:56:34: 13000000 INFO @ Sat, 15 Jan 2022 18:56:35: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:38: 14000000 INFO @ Sat, 15 Jan 2022 18:56:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:39: 7000000 INFO @ Sat, 15 Jan 2022 18:56:43: 15000000 INFO @ Sat, 15 Jan 2022 18:56:43: 1000000 INFO @ Sat, 15 Jan 2022 18:56:44: 8000000 INFO @ Sat, 15 Jan 2022 18:56:47: 16000000 INFO @ Sat, 15 Jan 2022 18:56:48: 2000000 INFO @ Sat, 15 Jan 2022 18:56:48: 9000000 INFO @ Sat, 15 Jan 2022 18:56:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:56:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:56:51: #1 total tags in treatment: 7946173 INFO @ Sat, 15 Jan 2022 18:56:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:56:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:56:51: #1 tags after filtering in treatment: 5527791 INFO @ Sat, 15 Jan 2022 18:56:51: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 18:56:51: #1 finished! INFO @ Sat, 15 Jan 2022 18:56:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:56:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:56:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:56:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:56:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:56:52: 3000000 INFO @ Sat, 15 Jan 2022 18:56:52: 10000000 INFO @ Sat, 15 Jan 2022 18:56:56: 4000000 INFO @ Sat, 15 Jan 2022 18:56:57: 11000000 INFO @ Sat, 15 Jan 2022 18:57:01: 5000000 INFO @ Sat, 15 Jan 2022 18:57:01: 12000000 INFO @ Sat, 15 Jan 2022 18:57:05: 6000000 INFO @ Sat, 15 Jan 2022 18:57:05: 13000000 INFO @ Sat, 15 Jan 2022 18:57:10: 7000000 INFO @ Sat, 15 Jan 2022 18:57:10: 14000000 INFO @ Sat, 15 Jan 2022 18:57:14: 8000000 INFO @ Sat, 15 Jan 2022 18:57:14: 15000000 INFO @ Sat, 15 Jan 2022 18:57:18: 9000000 INFO @ Sat, 15 Jan 2022 18:57:19: 16000000 INFO @ Sat, 15 Jan 2022 18:57:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:57:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:57:22: #1 total tags in treatment: 7946173 INFO @ Sat, 15 Jan 2022 18:57:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:57:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:57:22: #1 tags after filtering in treatment: 5527791 INFO @ Sat, 15 Jan 2022 18:57:22: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 18:57:22: #1 finished! INFO @ Sat, 15 Jan 2022 18:57:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:57:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:57:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:57:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:57:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:57:23: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:57:27: 11000000 INFO @ Sat, 15 Jan 2022 18:57:31: 12000000 INFO @ Sat, 15 Jan 2022 18:57:36: 13000000 INFO @ Sat, 15 Jan 2022 18:57:40: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:57:44: 15000000 INFO @ Sat, 15 Jan 2022 18:57:49: 16000000 INFO @ Sat, 15 Jan 2022 18:57:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:57:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:57:52: #1 total tags in treatment: 7946173 INFO @ Sat, 15 Jan 2022 18:57:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:57:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:57:52: #1 tags after filtering in treatment: 5527791 INFO @ Sat, 15 Jan 2022 18:57:52: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 18:57:52: #1 finished! INFO @ Sat, 15 Jan 2022 18:57:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:57:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:57:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:57:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:57:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592373/SRX10592373.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling