Job ID = 14520171 SRX = SRX10592372 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:29:51 prefetch.2.10.7: 1) Downloading 'SRR14227556'... 2022-01-15T09:29:51 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:31:08 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:31:08 prefetch.2.10.7: 1) 'SRR14227556' was downloaded successfully 2022-01-15T09:31:08 prefetch.2.10.7: 'SRR14227556' has 0 unresolved dependencies Read 24867111 spots for SRR14227556/SRR14227556.sra Written 24867111 spots for SRR14227556/SRR14227556.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:39 24867111 reads; of these: 24867111 (100.00%) were paired; of these: 1250280 (5.03%) aligned concordantly 0 times 18576311 (74.70%) aligned concordantly exactly 1 time 5040520 (20.27%) aligned concordantly >1 times ---- 1250280 pairs aligned concordantly 0 times; of these: 76610 (6.13%) aligned discordantly 1 time ---- 1173670 pairs aligned 0 times concordantly or discordantly; of these: 2347340 mates make up the pairs; of these: 1860449 (79.26%) aligned 0 times 368693 (15.71%) aligned exactly 1 time 118198 (5.04%) aligned >1 times 96.26% overall alignment rate Time searching: 00:13:39 Overall time: 00:13:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 9729335 / 23688321 = 0.4107 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:11: 1000000 INFO @ Sat, 15 Jan 2022 18:56:17: 2000000 INFO @ Sat, 15 Jan 2022 18:56:22: 3000000 INFO @ Sat, 15 Jan 2022 18:56:27: 4000000 INFO @ Sat, 15 Jan 2022 18:56:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:37: 6000000 INFO @ Sat, 15 Jan 2022 18:56:41: 1000000 INFO @ Sat, 15 Jan 2022 18:56:42: 7000000 INFO @ Sat, 15 Jan 2022 18:56:46: 2000000 INFO @ Sat, 15 Jan 2022 18:56:47: 8000000 INFO @ Sat, 15 Jan 2022 18:56:51: 3000000 INFO @ Sat, 15 Jan 2022 18:56:52: 9000000 INFO @ Sat, 15 Jan 2022 18:56:56: 4000000 INFO @ Sat, 15 Jan 2022 18:56:57: 10000000 INFO @ Sat, 15 Jan 2022 18:57:01: 5000000 INFO @ Sat, 15 Jan 2022 18:57:02: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:06: 6000000 INFO @ Sat, 15 Jan 2022 18:57:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:07: 12000000 INFO @ Sat, 15 Jan 2022 18:57:11: 7000000 INFO @ Sat, 15 Jan 2022 18:57:12: 1000000 INFO @ Sat, 15 Jan 2022 18:57:12: 13000000 INFO @ Sat, 15 Jan 2022 18:57:16: 8000000 INFO @ Sat, 15 Jan 2022 18:57:17: 2000000 INFO @ Sat, 15 Jan 2022 18:57:17: 14000000 INFO @ Sat, 15 Jan 2022 18:57:21: 9000000 INFO @ Sat, 15 Jan 2022 18:57:22: 3000000 INFO @ Sat, 15 Jan 2022 18:57:23: 15000000 INFO @ Sat, 15 Jan 2022 18:57:26: 10000000 INFO @ Sat, 15 Jan 2022 18:57:27: 4000000 INFO @ Sat, 15 Jan 2022 18:57:28: 16000000 INFO @ Sat, 15 Jan 2022 18:57:31: 11000000 INFO @ Sat, 15 Jan 2022 18:57:32: 5000000 INFO @ Sat, 15 Jan 2022 18:57:33: 17000000 INFO @ Sat, 15 Jan 2022 18:57:36: 12000000 INFO @ Sat, 15 Jan 2022 18:57:37: 6000000 INFO @ Sat, 15 Jan 2022 18:57:38: 18000000 INFO @ Sat, 15 Jan 2022 18:57:41: 13000000 INFO @ Sat, 15 Jan 2022 18:57:43: 7000000 INFO @ Sat, 15 Jan 2022 18:57:43: 19000000 INFO @ Sat, 15 Jan 2022 18:57:47: 14000000 INFO @ Sat, 15 Jan 2022 18:57:48: 8000000 INFO @ Sat, 15 Jan 2022 18:57:48: 20000000 INFO @ Sat, 15 Jan 2022 18:57:52: 15000000 INFO @ Sat, 15 Jan 2022 18:57:53: 9000000 INFO @ Sat, 15 Jan 2022 18:57:53: 21000000 INFO @ Sat, 15 Jan 2022 18:57:57: 16000000 INFO @ Sat, 15 Jan 2022 18:57:58: 10000000 INFO @ Sat, 15 Jan 2022 18:57:58: 22000000 INFO @ Sat, 15 Jan 2022 18:58:02: 17000000 INFO @ Sat, 15 Jan 2022 18:58:03: 11000000 INFO @ Sat, 15 Jan 2022 18:58:03: 23000000 INFO @ Sat, 15 Jan 2022 18:58:07: 18000000 INFO @ Sat, 15 Jan 2022 18:58:08: 12000000 INFO @ Sat, 15 Jan 2022 18:58:09: 24000000 INFO @ Sat, 15 Jan 2022 18:58:12: 19000000 INFO @ Sat, 15 Jan 2022 18:58:13: 13000000 INFO @ Sat, 15 Jan 2022 18:58:14: 25000000 INFO @ Sat, 15 Jan 2022 18:58:17: 20000000 INFO @ Sat, 15 Jan 2022 18:58:18: 14000000 INFO @ Sat, 15 Jan 2022 18:58:19: 26000000 INFO @ Sat, 15 Jan 2022 18:58:22: 21000000 INFO @ Sat, 15 Jan 2022 18:58:23: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:58:24: 27000000 INFO @ Sat, 15 Jan 2022 18:58:27: 22000000 INFO @ Sat, 15 Jan 2022 18:58:28: 16000000 INFO @ Sat, 15 Jan 2022 18:58:29: 28000000 INFO @ Sat, 15 Jan 2022 18:58:32: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:32: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:32: #1 total tags in treatment: 13916654 INFO @ Sat, 15 Jan 2022 18:58:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:32: 23000000 INFO @ Sat, 15 Jan 2022 18:58:32: #1 tags after filtering in treatment: 9088228 INFO @ Sat, 15 Jan 2022 18:58:32: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:58:32: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:58:33: 17000000 INFO @ Sat, 15 Jan 2022 18:58:36: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:58:38: 18000000 INFO @ Sat, 15 Jan 2022 18:58:42: 25000000 INFO @ Sat, 15 Jan 2022 18:58:43: 19000000 INFO @ Sat, 15 Jan 2022 18:58:47: 26000000 INFO @ Sat, 15 Jan 2022 18:58:48: 20000000 INFO @ Sat, 15 Jan 2022 18:58:52: 27000000 INFO @ Sat, 15 Jan 2022 18:58:53: 21000000 INFO @ Sat, 15 Jan 2022 18:58:57: 28000000 INFO @ Sat, 15 Jan 2022 18:58:58: 22000000 INFO @ Sat, 15 Jan 2022 18:58:59: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:59: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:59: #1 total tags in treatment: 13916654 INFO @ Sat, 15 Jan 2022 18:58:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:59: #1 tags after filtering in treatment: 9088228 INFO @ Sat, 15 Jan 2022 18:58:59: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:58:59: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:59:03: 23000000 INFO @ Sat, 15 Jan 2022 18:59:08: 24000000 INFO @ Sat, 15 Jan 2022 18:59:13: 25000000 INFO @ Sat, 15 Jan 2022 18:59:18: 26000000 INFO @ Sat, 15 Jan 2022 18:59:23: 27000000 INFO @ Sat, 15 Jan 2022 18:59:28: 28000000 INFO @ Sat, 15 Jan 2022 18:59:30: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:59:30: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:59:30: #1 total tags in treatment: 13916654 INFO @ Sat, 15 Jan 2022 18:59:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:30: #1 tags after filtering in treatment: 9088228 INFO @ Sat, 15 Jan 2022 18:59:30: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 18:59:30: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592372/SRX10592372.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling