Job ID = 14520170 SRX = SRX10592371 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:29:51 prefetch.2.10.7: 1) Downloading 'SRR14227555'... 2022-01-15T09:29:51 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:31:18 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:31:18 prefetch.2.10.7: 1) 'SRR14227555' was downloaded successfully 2022-01-15T09:31:18 prefetch.2.10.7: 'SRR14227555' has 0 unresolved dependencies Read 25940137 spots for SRR14227555/SRR14227555.sra Written 25940137 spots for SRR14227555/SRR14227555.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:29 25940137 reads; of these: 25940137 (100.00%) were paired; of these: 1266849 (4.88%) aligned concordantly 0 times 19466301 (75.04%) aligned concordantly exactly 1 time 5206987 (20.07%) aligned concordantly >1 times ---- 1266849 pairs aligned concordantly 0 times; of these: 53133 (4.19%) aligned discordantly 1 time ---- 1213716 pairs aligned 0 times concordantly or discordantly; of these: 2427432 mates make up the pairs; of these: 1909647 (78.67%) aligned 0 times 416061 (17.14%) aligned exactly 1 time 101724 (4.19%) aligned >1 times 96.32% overall alignment rate Time searching: 00:14:29 Overall time: 00:14:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 11982426 / 24721928 = 0.4847 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:06: 1000000 INFO @ Sat, 15 Jan 2022 18:57:10: 2000000 INFO @ Sat, 15 Jan 2022 18:57:15: 3000000 INFO @ Sat, 15 Jan 2022 18:57:19: 4000000 INFO @ Sat, 15 Jan 2022 18:57:24: 5000000 INFO @ Sat, 15 Jan 2022 18:57:28: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:33: 7000000 INFO @ Sat, 15 Jan 2022 18:57:37: 1000000 INFO @ Sat, 15 Jan 2022 18:57:38: 8000000 INFO @ Sat, 15 Jan 2022 18:57:43: 2000000 INFO @ Sat, 15 Jan 2022 18:57:43: 9000000 INFO @ Sat, 15 Jan 2022 18:57:49: 10000000 INFO @ Sat, 15 Jan 2022 18:57:49: 3000000 INFO @ Sat, 15 Jan 2022 18:57:54: 11000000 INFO @ Sat, 15 Jan 2022 18:57:55: 4000000 INFO @ Sat, 15 Jan 2022 18:57:59: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:58:01: 5000000 INFO @ Sat, 15 Jan 2022 18:58:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:58:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:58:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:58:04: 13000000 INFO @ Sat, 15 Jan 2022 18:58:07: 1000000 INFO @ Sat, 15 Jan 2022 18:58:07: 6000000 INFO @ Sat, 15 Jan 2022 18:58:09: 14000000 INFO @ Sat, 15 Jan 2022 18:58:12: 2000000 INFO @ Sat, 15 Jan 2022 18:58:13: 7000000 INFO @ Sat, 15 Jan 2022 18:58:14: 15000000 INFO @ Sat, 15 Jan 2022 18:58:17: 3000000 INFO @ Sat, 15 Jan 2022 18:58:19: 8000000 INFO @ Sat, 15 Jan 2022 18:58:19: 16000000 INFO @ Sat, 15 Jan 2022 18:58:22: 4000000 INFO @ Sat, 15 Jan 2022 18:58:24: 17000000 INFO @ Sat, 15 Jan 2022 18:58:25: 9000000 INFO @ Sat, 15 Jan 2022 18:58:27: 5000000 INFO @ Sat, 15 Jan 2022 18:58:30: 18000000 INFO @ Sat, 15 Jan 2022 18:58:31: 10000000 INFO @ Sat, 15 Jan 2022 18:58:32: 6000000 INFO @ Sat, 15 Jan 2022 18:58:35: 19000000 INFO @ Sat, 15 Jan 2022 18:58:37: 11000000 INFO @ Sat, 15 Jan 2022 18:58:38: 7000000 INFO @ Sat, 15 Jan 2022 18:58:40: 20000000 INFO @ Sat, 15 Jan 2022 18:58:43: 8000000 INFO @ Sat, 15 Jan 2022 18:58:44: 12000000 INFO @ Sat, 15 Jan 2022 18:58:45: 21000000 INFO @ Sat, 15 Jan 2022 18:58:48: 9000000 INFO @ Sat, 15 Jan 2022 18:58:50: 13000000 INFO @ Sat, 15 Jan 2022 18:58:50: 22000000 INFO @ Sat, 15 Jan 2022 18:58:53: 10000000 INFO @ Sat, 15 Jan 2022 18:58:55: 14000000 INFO @ Sat, 15 Jan 2022 18:58:56: 23000000 INFO @ Sat, 15 Jan 2022 18:58:58: 11000000 INFO @ Sat, 15 Jan 2022 18:59:01: 24000000 INFO @ Sat, 15 Jan 2022 18:59:01: 15000000 INFO @ Sat, 15 Jan 2022 18:59:04: 12000000 INFO @ Sat, 15 Jan 2022 18:59:07: 25000000 INFO @ Sat, 15 Jan 2022 18:59:07: 16000000 INFO @ Sat, 15 Jan 2022 18:59:09: 13000000 INFO @ Sat, 15 Jan 2022 18:59:12: 26000000 INFO @ Sat, 15 Jan 2022 18:59:12: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:59:12: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:59:12: #1 total tags in treatment: 12712626 INFO @ Sat, 15 Jan 2022 18:59:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:13: #1 tags after filtering in treatment: 8483131 INFO @ Sat, 15 Jan 2022 18:59:13: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 18:59:13: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:13: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:59:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:59:14: 14000000 INFO @ Sat, 15 Jan 2022 18:59:19: 18000000 INFO @ Sat, 15 Jan 2022 18:59:19: 15000000 INFO @ Sat, 15 Jan 2022 18:59:24: 16000000 INFO @ Sat, 15 Jan 2022 18:59:25: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:59:29: 17000000 INFO @ Sat, 15 Jan 2022 18:59:31: 20000000 INFO @ Sat, 15 Jan 2022 18:59:34: 18000000 INFO @ Sat, 15 Jan 2022 18:59:37: 21000000 INFO @ Sat, 15 Jan 2022 18:59:40: 19000000 INFO @ Sat, 15 Jan 2022 18:59:42: 22000000 INFO @ Sat, 15 Jan 2022 18:59:45: 20000000 INFO @ Sat, 15 Jan 2022 18:59:48: 23000000 INFO @ Sat, 15 Jan 2022 18:59:50: 21000000 INFO @ Sat, 15 Jan 2022 18:59:54: 24000000 INFO @ Sat, 15 Jan 2022 18:59:55: 22000000 INFO @ Sat, 15 Jan 2022 19:00:00: 25000000 INFO @ Sat, 15 Jan 2022 19:00:00: 23000000 INFO @ Sat, 15 Jan 2022 19:00:05: 24000000 INFO @ Sat, 15 Jan 2022 19:00:05: 26000000 INFO @ Sat, 15 Jan 2022 19:00:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:00:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:00:06: #1 total tags in treatment: 12712626 INFO @ Sat, 15 Jan 2022 19:00:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:00:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:00:06: #1 tags after filtering in treatment: 8483131 INFO @ Sat, 15 Jan 2022 19:00:06: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 19:00:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:00:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:00:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:00:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:00:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:00:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:00:10: 25000000 INFO @ Sat, 15 Jan 2022 19:00:15: 26000000 INFO @ Sat, 15 Jan 2022 19:00:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:00:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:00:15: #1 total tags in treatment: 12712626 INFO @ Sat, 15 Jan 2022 19:00:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:00:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:00:15: #1 tags after filtering in treatment: 8483131 INFO @ Sat, 15 Jan 2022 19:00:15: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 19:00:15: #1 finished! INFO @ Sat, 15 Jan 2022 19:00:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:00:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:00:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:00:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:00:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592371/SRX10592371.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling