Job ID = 14520168 SRX = SRX10592369 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:29:36 prefetch.2.10.7: 1) Downloading 'SRR14227553'... 2022-01-15T09:29:36 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:30:41 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:30:41 prefetch.2.10.7: 1) 'SRR14227553' was downloaded successfully 2022-01-15T09:30:41 prefetch.2.10.7: 'SRR14227553' has 0 unresolved dependencies Read 24775210 spots for SRR14227553/SRR14227553.sra Written 24775210 spots for SRR14227553/SRR14227553.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:54 24775210 reads; of these: 24775210 (100.00%) were paired; of these: 973433 (3.93%) aligned concordantly 0 times 21255898 (85.80%) aligned concordantly exactly 1 time 2545879 (10.28%) aligned concordantly >1 times ---- 973433 pairs aligned concordantly 0 times; of these: 118943 (12.22%) aligned discordantly 1 time ---- 854490 pairs aligned 0 times concordantly or discordantly; of these: 1708980 mates make up the pairs; of these: 1309868 (76.65%) aligned 0 times 309359 (18.10%) aligned exactly 1 time 89753 (5.25%) aligned >1 times 97.36% overall alignment rate Time searching: 00:20:54 Overall time: 00:20:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5655140 / 23915242 = 0.2365 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:06:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:06:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:06:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:06:49: 1000000 INFO @ Sat, 15 Jan 2022 19:06:53: 2000000 INFO @ Sat, 15 Jan 2022 19:06:57: 3000000 INFO @ Sat, 15 Jan 2022 19:07:02: 4000000 INFO @ Sat, 15 Jan 2022 19:07:06: 5000000 INFO @ Sat, 15 Jan 2022 19:07:10: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:07:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:07:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:07:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:07:15: 7000000 INFO @ Sat, 15 Jan 2022 19:07:19: 1000000 INFO @ Sat, 15 Jan 2022 19:07:19: 8000000 INFO @ Sat, 15 Jan 2022 19:07:24: 2000000 INFO @ Sat, 15 Jan 2022 19:07:24: 9000000 INFO @ Sat, 15 Jan 2022 19:07:29: 3000000 INFO @ Sat, 15 Jan 2022 19:07:29: 10000000 INFO @ Sat, 15 Jan 2022 19:07:33: 4000000 INFO @ Sat, 15 Jan 2022 19:07:33: 11000000 INFO @ Sat, 15 Jan 2022 19:07:38: 5000000 INFO @ Sat, 15 Jan 2022 19:07:38: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:07:43: 6000000 INFO @ Sat, 15 Jan 2022 19:07:43: 13000000 INFO @ Sat, 15 Jan 2022 19:07:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:07:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:07:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:07:47: 7000000 INFO @ Sat, 15 Jan 2022 19:07:47: 14000000 INFO @ Sat, 15 Jan 2022 19:07:49: 1000000 INFO @ Sat, 15 Jan 2022 19:07:52: 8000000 INFO @ Sat, 15 Jan 2022 19:07:52: 15000000 INFO @ Sat, 15 Jan 2022 19:07:53: 2000000 INFO @ Sat, 15 Jan 2022 19:07:57: 9000000 INFO @ Sat, 15 Jan 2022 19:07:57: 16000000 INFO @ Sat, 15 Jan 2022 19:07:58: 3000000 INFO @ Sat, 15 Jan 2022 19:08:01: 10000000 INFO @ Sat, 15 Jan 2022 19:08:02: 17000000 INFO @ Sat, 15 Jan 2022 19:08:03: 4000000 INFO @ Sat, 15 Jan 2022 19:08:06: 11000000 INFO @ Sat, 15 Jan 2022 19:08:06: 18000000 INFO @ Sat, 15 Jan 2022 19:08:07: 5000000 INFO @ Sat, 15 Jan 2022 19:08:11: 12000000 INFO @ Sat, 15 Jan 2022 19:08:11: 19000000 INFO @ Sat, 15 Jan 2022 19:08:12: 6000000 INFO @ Sat, 15 Jan 2022 19:08:16: 13000000 INFO @ Sat, 15 Jan 2022 19:08:16: 20000000 INFO @ Sat, 15 Jan 2022 19:08:16: 7000000 INFO @ Sat, 15 Jan 2022 19:08:20: 14000000 INFO @ Sat, 15 Jan 2022 19:08:21: 21000000 INFO @ Sat, 15 Jan 2022 19:08:21: 8000000 INFO @ Sat, 15 Jan 2022 19:08:25: 15000000 INFO @ Sat, 15 Jan 2022 19:08:25: 9000000 INFO @ Sat, 15 Jan 2022 19:08:25: 22000000 INFO @ Sat, 15 Jan 2022 19:08:30: 16000000 INFO @ Sat, 15 Jan 2022 19:08:30: 10000000 INFO @ Sat, 15 Jan 2022 19:08:30: 23000000 INFO @ Sat, 15 Jan 2022 19:08:34: 11000000 INFO @ Sat, 15 Jan 2022 19:08:34: 17000000 INFO @ Sat, 15 Jan 2022 19:08:35: 24000000 INFO @ Sat, 15 Jan 2022 19:08:39: 12000000 INFO @ Sat, 15 Jan 2022 19:08:39: 18000000 INFO @ Sat, 15 Jan 2022 19:08:39: 25000000 INFO @ Sat, 15 Jan 2022 19:08:43: 13000000 INFO @ Sat, 15 Jan 2022 19:08:44: 19000000 INFO @ Sat, 15 Jan 2022 19:08:44: 26000000 INFO @ Sat, 15 Jan 2022 19:08:48: 14000000 INFO @ Sat, 15 Jan 2022 19:08:49: 20000000 INFO @ Sat, 15 Jan 2022 19:08:49: 27000000 INFO @ Sat, 15 Jan 2022 19:08:53: 15000000 INFO @ Sat, 15 Jan 2022 19:08:53: 21000000 INFO @ Sat, 15 Jan 2022 19:08:54: 28000000 INFO @ Sat, 15 Jan 2022 19:08:57: 16000000 INFO @ Sat, 15 Jan 2022 19:08:58: 22000000 INFO @ Sat, 15 Jan 2022 19:08:58: 29000000 INFO @ Sat, 15 Jan 2022 19:09:02: 17000000 INFO @ Sat, 15 Jan 2022 19:09:03: 23000000 INFO @ Sat, 15 Jan 2022 19:09:03: 30000000 INFO @ Sat, 15 Jan 2022 19:09:07: 18000000 INFO @ Sat, 15 Jan 2022 19:09:07: 24000000 INFO @ Sat, 15 Jan 2022 19:09:08: 31000000 INFO @ Sat, 15 Jan 2022 19:09:11: 19000000 INFO @ Sat, 15 Jan 2022 19:09:12: 25000000 INFO @ Sat, 15 Jan 2022 19:09:12: 32000000 INFO @ Sat, 15 Jan 2022 19:09:16: 20000000 INFO @ Sat, 15 Jan 2022 19:09:17: 26000000 INFO @ Sat, 15 Jan 2022 19:09:17: 33000000 INFO @ Sat, 15 Jan 2022 19:09:20: 21000000 INFO @ Sat, 15 Jan 2022 19:09:21: 27000000 INFO @ Sat, 15 Jan 2022 19:09:22: 34000000 INFO @ Sat, 15 Jan 2022 19:09:25: 22000000 INFO @ Sat, 15 Jan 2022 19:09:26: 28000000 INFO @ Sat, 15 Jan 2022 19:09:27: 35000000 INFO @ Sat, 15 Jan 2022 19:09:29: 23000000 INFO @ Sat, 15 Jan 2022 19:09:31: 29000000 INFO @ Sat, 15 Jan 2022 19:09:31: 36000000 INFO @ Sat, 15 Jan 2022 19:09:34: 24000000 INFO @ Sat, 15 Jan 2022 19:09:35: 30000000 INFO @ Sat, 15 Jan 2022 19:09:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:09:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:09:36: #1 total tags in treatment: 18174175 INFO @ Sat, 15 Jan 2022 19:09:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:09:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:09:36: #1 tags after filtering in treatment: 9682594 INFO @ Sat, 15 Jan 2022 19:09:36: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:09:36: #1 finished! INFO @ Sat, 15 Jan 2022 19:09:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:09:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:09:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:09:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:09:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:09:38: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:09:39: 31000000 INFO @ Sat, 15 Jan 2022 19:09:43: 26000000 INFO @ Sat, 15 Jan 2022 19:09:44: 32000000 INFO @ Sat, 15 Jan 2022 19:09:47: 27000000 INFO @ Sat, 15 Jan 2022 19:09:48: 33000000 INFO @ Sat, 15 Jan 2022 19:09:52: 28000000 INFO @ Sat, 15 Jan 2022 19:09:52: 34000000 INFO @ Sat, 15 Jan 2022 19:09:56: 29000000 INFO @ Sat, 15 Jan 2022 19:09:56: 35000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:10:01: 36000000 INFO @ Sat, 15 Jan 2022 19:10:01: 30000000 INFO @ Sat, 15 Jan 2022 19:10:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:10:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:10:05: #1 total tags in treatment: 18174175 INFO @ Sat, 15 Jan 2022 19:10:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:10:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:10:05: #1 tags after filtering in treatment: 9682594 INFO @ Sat, 15 Jan 2022 19:10:05: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:10:05: #1 finished! INFO @ Sat, 15 Jan 2022 19:10:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:10:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:10:05: 31000000 INFO @ Sat, 15 Jan 2022 19:10:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:10:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:10:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:10:10: 32000000 INFO @ Sat, 15 Jan 2022 19:10:14: 33000000 INFO @ Sat, 15 Jan 2022 19:10:19: 34000000 INFO @ Sat, 15 Jan 2022 19:10:23: 35000000 INFO @ Sat, 15 Jan 2022 19:10:28: 36000000 INFO @ Sat, 15 Jan 2022 19:10:32: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:10:32: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:10:32: #1 total tags in treatment: 18174175 INFO @ Sat, 15 Jan 2022 19:10:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:10:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:10:33: #1 tags after filtering in treatment: 9682594 INFO @ Sat, 15 Jan 2022 19:10:33: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:10:33: #1 finished! INFO @ Sat, 15 Jan 2022 19:10:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:10:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:10:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:10:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:10:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592369/SRX10592369.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling