Job ID = 14520167 SRX = SRX10592368 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:29:21 prefetch.2.10.7: 1) Downloading 'SRR14227552'... 2022-01-15T09:29:21 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:30:47 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:30:47 prefetch.2.10.7: 1) 'SRR14227552' was downloaded successfully 2022-01-15T09:30:47 prefetch.2.10.7: 'SRR14227552' has 0 unresolved dependencies Read 23070073 spots for SRR14227552/SRR14227552.sra Written 23070073 spots for SRR14227552/SRR14227552.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:05 23070073 reads; of these: 23070073 (100.00%) were paired; of these: 911284 (3.95%) aligned concordantly 0 times 19546433 (84.73%) aligned concordantly exactly 1 time 2612356 (11.32%) aligned concordantly >1 times ---- 911284 pairs aligned concordantly 0 times; of these: 41874 (4.60%) aligned discordantly 1 time ---- 869410 pairs aligned 0 times concordantly or discordantly; of these: 1738820 mates make up the pairs; of these: 1394807 (80.22%) aligned 0 times 285749 (16.43%) aligned exactly 1 time 58264 (3.35%) aligned >1 times 96.98% overall alignment rate Time searching: 00:12:05 Overall time: 00:12:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 6266402 / 22195310 = 0.2823 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:53:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:53:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:53:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:54:01: 1000000 INFO @ Sat, 15 Jan 2022 18:54:06: 2000000 INFO @ Sat, 15 Jan 2022 18:54:11: 3000000 INFO @ Sat, 15 Jan 2022 18:54:16: 4000000 INFO @ Sat, 15 Jan 2022 18:54:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:54:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:54:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:54:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:54:26: 6000000 INFO @ Sat, 15 Jan 2022 18:54:32: 7000000 INFO @ Sat, 15 Jan 2022 18:54:33: 1000000 INFO @ Sat, 15 Jan 2022 18:54:39: 8000000 INFO @ Sat, 15 Jan 2022 18:54:40: 2000000 INFO @ Sat, 15 Jan 2022 18:54:45: 9000000 INFO @ Sat, 15 Jan 2022 18:54:47: 3000000 INFO @ Sat, 15 Jan 2022 18:54:51: 10000000 INFO @ Sat, 15 Jan 2022 18:54:54: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:54:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:54:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:54:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:54:58: 11000000 INFO @ Sat, 15 Jan 2022 18:55:01: 5000000 INFO @ Sat, 15 Jan 2022 18:55:03: 1000000 INFO @ Sat, 15 Jan 2022 18:55:04: 12000000 INFO @ Sat, 15 Jan 2022 18:55:08: 6000000 INFO @ Sat, 15 Jan 2022 18:55:10: 2000000 INFO @ Sat, 15 Jan 2022 18:55:10: 13000000 INFO @ Sat, 15 Jan 2022 18:55:15: 7000000 INFO @ Sat, 15 Jan 2022 18:55:17: 14000000 INFO @ Sat, 15 Jan 2022 18:55:17: 3000000 INFO @ Sat, 15 Jan 2022 18:55:23: 8000000 INFO @ Sat, 15 Jan 2022 18:55:23: 15000000 INFO @ Sat, 15 Jan 2022 18:55:24: 4000000 INFO @ Sat, 15 Jan 2022 18:55:30: 9000000 INFO @ Sat, 15 Jan 2022 18:55:30: 16000000 INFO @ Sat, 15 Jan 2022 18:55:31: 5000000 INFO @ Sat, 15 Jan 2022 18:55:36: 17000000 INFO @ Sat, 15 Jan 2022 18:55:37: 10000000 INFO @ Sat, 15 Jan 2022 18:55:38: 6000000 INFO @ Sat, 15 Jan 2022 18:55:43: 18000000 INFO @ Sat, 15 Jan 2022 18:55:44: 11000000 INFO @ Sat, 15 Jan 2022 18:55:45: 7000000 INFO @ Sat, 15 Jan 2022 18:55:49: 19000000 INFO @ Sat, 15 Jan 2022 18:55:51: 12000000 INFO @ Sat, 15 Jan 2022 18:55:52: 8000000 INFO @ Sat, 15 Jan 2022 18:55:56: 20000000 INFO @ Sat, 15 Jan 2022 18:55:58: 13000000 INFO @ Sat, 15 Jan 2022 18:55:59: 9000000 INFO @ Sat, 15 Jan 2022 18:56:02: 21000000 INFO @ Sat, 15 Jan 2022 18:56:05: 14000000 INFO @ Sat, 15 Jan 2022 18:56:06: 10000000 INFO @ Sat, 15 Jan 2022 18:56:08: 22000000 INFO @ Sat, 15 Jan 2022 18:56:12: 15000000 INFO @ Sat, 15 Jan 2022 18:56:13: 11000000 INFO @ Sat, 15 Jan 2022 18:56:15: 23000000 INFO @ Sat, 15 Jan 2022 18:56:19: 16000000 INFO @ Sat, 15 Jan 2022 18:56:21: 12000000 INFO @ Sat, 15 Jan 2022 18:56:21: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:56:26: 17000000 INFO @ Sat, 15 Jan 2022 18:56:28: 25000000 INFO @ Sat, 15 Jan 2022 18:56:28: 13000000 INFO @ Sat, 15 Jan 2022 18:56:33: 18000000 INFO @ Sat, 15 Jan 2022 18:56:34: 26000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:56:35: 14000000 INFO @ Sat, 15 Jan 2022 18:56:40: 19000000 INFO @ Sat, 15 Jan 2022 18:56:40: 27000000 INFO @ Sat, 15 Jan 2022 18:56:43: 15000000 INFO @ Sat, 15 Jan 2022 18:56:47: 28000000 INFO @ Sat, 15 Jan 2022 18:56:47: 20000000 INFO @ Sat, 15 Jan 2022 18:56:50: 16000000 INFO @ Sat, 15 Jan 2022 18:56:53: 29000000 INFO @ Sat, 15 Jan 2022 18:56:55: 21000000 INFO @ Sat, 15 Jan 2022 18:56:57: 17000000 INFO @ Sat, 15 Jan 2022 18:56:59: 30000000 INFO @ Sat, 15 Jan 2022 18:57:02: 22000000 INFO @ Sat, 15 Jan 2022 18:57:04: 18000000 INFO @ Sat, 15 Jan 2022 18:57:06: 31000000 INFO @ Sat, 15 Jan 2022 18:57:09: 23000000 INFO @ Sat, 15 Jan 2022 18:57:11: 19000000 INFO @ Sat, 15 Jan 2022 18:57:12: 32000000 INFO @ Sat, 15 Jan 2022 18:57:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:57:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:57:14: #1 total tags in treatment: 15901742 INFO @ Sat, 15 Jan 2022 18:57:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:57:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:57:14: #1 tags after filtering in treatment: 8473795 INFO @ Sat, 15 Jan 2022 18:57:14: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 18:57:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:57:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:57:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:57:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:57:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:57:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:57:16: 24000000 INFO @ Sat, 15 Jan 2022 18:57:19: 20000000 INFO @ Sat, 15 Jan 2022 18:57:23: 25000000 INFO @ Sat, 15 Jan 2022 18:57:26: 21000000 INFO @ Sat, 15 Jan 2022 18:57:30: 26000000 INFO @ Sat, 15 Jan 2022 18:57:33: 22000000 INFO @ Sat, 15 Jan 2022 18:57:37: 27000000 INFO @ Sat, 15 Jan 2022 18:57:40: 23000000 INFO @ Sat, 15 Jan 2022 18:57:44: 28000000 INFO @ Sat, 15 Jan 2022 18:57:47: 24000000 INFO @ Sat, 15 Jan 2022 18:57:51: 29000000 INFO @ Sat, 15 Jan 2022 18:57:54: 25000000 INFO @ Sat, 15 Jan 2022 18:57:58: 30000000 INFO @ Sat, 15 Jan 2022 18:58:01: 26000000 INFO @ Sat, 15 Jan 2022 18:58:05: 31000000 INFO @ Sat, 15 Jan 2022 18:58:08: 27000000 INFO @ Sat, 15 Jan 2022 18:58:11: 32000000 INFO @ Sat, 15 Jan 2022 18:58:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:13: #1 total tags in treatment: 15901742 INFO @ Sat, 15 Jan 2022 18:58:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:13: #1 tags after filtering in treatment: 8473795 INFO @ Sat, 15 Jan 2022 18:58:13: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 18:58:13: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:58:15: 28000000 INFO @ Sat, 15 Jan 2022 18:58:21: 29000000 INFO @ Sat, 15 Jan 2022 18:58:28: 30000000 INFO @ Sat, 15 Jan 2022 18:58:34: 31000000 INFO @ Sat, 15 Jan 2022 18:58:40: 32000000 INFO @ Sat, 15 Jan 2022 18:58:42: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:42: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:42: #1 total tags in treatment: 15901742 INFO @ Sat, 15 Jan 2022 18:58:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:42: #1 tags after filtering in treatment: 8473795 INFO @ Sat, 15 Jan 2022 18:58:42: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 18:58:42: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592368/SRX10592368.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling