Job ID = 14520166 SRX = SRX10592367 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:29:22 prefetch.2.10.7: 1) Downloading 'SRR14227551'... 2022-01-15T09:29:22 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:30:27 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:30:27 prefetch.2.10.7: 1) 'SRR14227551' was downloaded successfully 2022-01-15T09:30:27 prefetch.2.10.7: 'SRR14227551' has 0 unresolved dependencies Read 23256663 spots for SRR14227551/SRR14227551.sra Written 23256663 spots for SRR14227551/SRR14227551.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:38 23256663 reads; of these: 23256663 (100.00%) were paired; of these: 969361 (4.17%) aligned concordantly 0 times 19453442 (83.65%) aligned concordantly exactly 1 time 2833860 (12.19%) aligned concordantly >1 times ---- 969361 pairs aligned concordantly 0 times; of these: 48306 (4.98%) aligned discordantly 1 time ---- 921055 pairs aligned 0 times concordantly or discordantly; of these: 1842110 mates make up the pairs; of these: 1433526 (77.82%) aligned 0 times 342451 (18.59%) aligned exactly 1 time 66133 (3.59%) aligned >1 times 96.92% overall alignment rate Time searching: 00:13:38 Overall time: 00:13:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 6449680 / 22329670 = 0.2888 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:28:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:28:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:28:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:28:52: 1000000 INFO @ Sat, 15 Jan 2022 19:28:58: 2000000 INFO @ Sat, 15 Jan 2022 19:29:04: 3000000 INFO @ Sat, 15 Jan 2022 19:29:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:29:16: 5000000 INFO @ Sat, 15 Jan 2022 19:29:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:29:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:29:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:29:22: 6000000 INFO @ Sat, 15 Jan 2022 19:29:23: 1000000 INFO @ Sat, 15 Jan 2022 19:29:28: 7000000 INFO @ Sat, 15 Jan 2022 19:29:29: 2000000 INFO @ Sat, 15 Jan 2022 19:29:34: 8000000 INFO @ Sat, 15 Jan 2022 19:29:35: 3000000 INFO @ Sat, 15 Jan 2022 19:29:40: 9000000 INFO @ Sat, 15 Jan 2022 19:29:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換中... INFO @ Sat, 15 Jan 2022 19:29:46: 10000000 INFO @ Sat, 15 Jan 2022 19:29:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:29:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:29:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:29:47: 5000000 INFO @ Sat, 15 Jan 2022 19:29:52: 1000000 INFO @ Sat, 15 Jan 2022 19:29:53: 11000000 INFO @ Sat, 15 Jan 2022 19:29:53: 6000000 INFO @ Sat, 15 Jan 2022 19:29:58: 2000000 INFO @ Sat, 15 Jan 2022 19:29:59: 12000000 INFO @ Sat, 15 Jan 2022 19:30:00: 7000000 INFO @ Sat, 15 Jan 2022 19:30:04: 3000000 INFO @ Sat, 15 Jan 2022 19:30:05: 13000000 INFO @ Sat, 15 Jan 2022 19:30:06: 8000000 INFO @ Sat, 15 Jan 2022 19:30:10: 4000000 INFO @ Sat, 15 Jan 2022 19:30:12: 14000000 INFO @ Sat, 15 Jan 2022 19:30:12: 9000000 INFO @ Sat, 15 Jan 2022 19:30:16: 5000000 INFO @ Sat, 15 Jan 2022 19:30:18: 15000000 INFO @ Sat, 15 Jan 2022 19:30:18: 10000000 INFO @ Sat, 15 Jan 2022 19:30:21: 6000000 INFO @ Sat, 15 Jan 2022 19:30:24: 16000000 INFO @ Sat, 15 Jan 2022 19:30:25: 11000000 INFO @ Sat, 15 Jan 2022 19:30:27: 7000000 INFO @ Sat, 15 Jan 2022 19:30:30: 17000000 INFO @ Sat, 15 Jan 2022 19:30:31: 12000000 INFO @ Sat, 15 Jan 2022 19:30:33: 8000000 INFO @ Sat, 15 Jan 2022 19:30:37: 18000000 INFO @ Sat, 15 Jan 2022 19:30:37: 13000000 INFO @ Sat, 15 Jan 2022 19:30:39: 9000000 INFO @ Sat, 15 Jan 2022 19:30:43: 19000000 INFO @ Sat, 15 Jan 2022 19:30:43: 14000000 INFO @ Sat, 15 Jan 2022 19:30:45: 10000000 INFO @ Sat, 15 Jan 2022 19:30:49: 20000000 INFO @ Sat, 15 Jan 2022 19:30:50: 15000000 INFO @ Sat, 15 Jan 2022 19:30:51: 11000000 INFO @ Sat, 15 Jan 2022 19:30:55: 21000000 INFO @ Sat, 15 Jan 2022 19:30:56: 16000000 INFO @ Sat, 15 Jan 2022 19:30:56: 12000000 INFO @ Sat, 15 Jan 2022 19:31:01: 22000000 INFO @ Sat, 15 Jan 2022 19:31:02: 17000000 INFO @ Sat, 15 Jan 2022 19:31:02: 13000000 INFO @ Sat, 15 Jan 2022 19:31:08: 23000000 INFO @ Sat, 15 Jan 2022 19:31:08: 14000000 INFO @ Sat, 15 Jan 2022 19:31:08: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:31:14: 15000000 INFO @ Sat, 15 Jan 2022 19:31:14: 24000000 INFO @ Sat, 15 Jan 2022 19:31:14: 19000000 INFO @ Sat, 15 Jan 2022 19:31:19: 16000000 INFO @ Sat, 15 Jan 2022 19:31:20: 25000000 INFO @ Sat, 15 Jan 2022 19:31:20: 20000000 INFO @ Sat, 15 Jan 2022 19:31:25: 17000000 INFO @ Sat, 15 Jan 2022 19:31:26: 26000000 INFO @ Sat, 15 Jan 2022 19:31:27: 21000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:31:31: 18000000 INFO @ Sat, 15 Jan 2022 19:31:33: 27000000 INFO @ Sat, 15 Jan 2022 19:31:33: 22000000 INFO @ Sat, 15 Jan 2022 19:31:37: 19000000 INFO @ Sat, 15 Jan 2022 19:31:39: 28000000 INFO @ Sat, 15 Jan 2022 19:31:39: 23000000 INFO @ Sat, 15 Jan 2022 19:31:42: 20000000 INFO @ Sat, 15 Jan 2022 19:31:45: 29000000 INFO @ Sat, 15 Jan 2022 19:31:45: 24000000 INFO @ Sat, 15 Jan 2022 19:31:48: 21000000 INFO @ Sat, 15 Jan 2022 19:31:51: 30000000 INFO @ Sat, 15 Jan 2022 19:31:51: 25000000 INFO @ Sat, 15 Jan 2022 19:31:54: 22000000 INFO @ Sat, 15 Jan 2022 19:31:57: 31000000 INFO @ Sat, 15 Jan 2022 19:31:58: 26000000 INFO @ Sat, 15 Jan 2022 19:31:59: 23000000 INFO @ Sat, 15 Jan 2022 19:32:03: 32000000 INFO @ Sat, 15 Jan 2022 19:32:04: 27000000 INFO @ Sat, 15 Jan 2022 19:32:05: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:32:05: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:32:05: #1 total tags in treatment: 15849527 INFO @ Sat, 15 Jan 2022 19:32:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:32:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:32:05: #1 tags after filtering in treatment: 8425497 INFO @ Sat, 15 Jan 2022 19:32:05: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:32:05: #1 finished! INFO @ Sat, 15 Jan 2022 19:32:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:32:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:32:05: 24000000 INFO @ Sat, 15 Jan 2022 19:32:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:32:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:32:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:32:10: 28000000 INFO @ Sat, 15 Jan 2022 19:32:11: 25000000 INFO @ Sat, 15 Jan 2022 19:32:16: 29000000 INFO @ Sat, 15 Jan 2022 19:32:17: 26000000 INFO @ Sat, 15 Jan 2022 19:32:22: 30000000 INFO @ Sat, 15 Jan 2022 19:32:22: 27000000 INFO @ Sat, 15 Jan 2022 19:32:28: 28000000 INFO @ Sat, 15 Jan 2022 19:32:28: 31000000 INFO @ Sat, 15 Jan 2022 19:32:33: 29000000 INFO @ Sat, 15 Jan 2022 19:32:34: 32000000 INFO @ Sat, 15 Jan 2022 19:32:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:32:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:32:35: #1 total tags in treatment: 15849527 INFO @ Sat, 15 Jan 2022 19:32:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:32:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:32:35: #1 tags after filtering in treatment: 8425497 INFO @ Sat, 15 Jan 2022 19:32:35: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:32:35: #1 finished! INFO @ Sat, 15 Jan 2022 19:32:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:32:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:32:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:32:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:32:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:32:39: 30000000 INFO @ Sat, 15 Jan 2022 19:32:44: 31000000 INFO @ Sat, 15 Jan 2022 19:32:49: 32000000 INFO @ Sat, 15 Jan 2022 19:32:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:32:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:32:50: #1 total tags in treatment: 15849527 INFO @ Sat, 15 Jan 2022 19:32:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:32:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:32:50: #1 tags after filtering in treatment: 8425497 INFO @ Sat, 15 Jan 2022 19:32:50: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 15 Jan 2022 19:32:50: #1 finished! INFO @ Sat, 15 Jan 2022 19:32:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:32:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:32:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:32:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:32:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592367/SRX10592367.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling