Job ID = 14520165 SRX = SRX10592366 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:29:06 prefetch.2.10.7: 1) Downloading 'SRR14227550'... 2022-01-15T09:29:06 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:30:09 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:30:09 prefetch.2.10.7: 1) 'SRR14227550' was downloaded successfully 2022-01-15T09:30:09 prefetch.2.10.7: 'SRR14227550' has 0 unresolved dependencies Read 22777805 spots for SRR14227550/SRR14227550.sra Written 22777805 spots for SRR14227550/SRR14227550.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:15 22777805 reads; of these: 22777805 (100.00%) were paired; of these: 941226 (4.13%) aligned concordantly 0 times 19291117 (84.69%) aligned concordantly exactly 1 time 2545462 (11.18%) aligned concordantly >1 times ---- 941226 pairs aligned concordantly 0 times; of these: 84308 (8.96%) aligned discordantly 1 time ---- 856918 pairs aligned 0 times concordantly or discordantly; of these: 1713836 mates make up the pairs; of these: 1355661 (79.10%) aligned 0 times 289672 (16.90%) aligned exactly 1 time 68503 (4.00%) aligned >1 times 97.02% overall alignment rate Time searching: 00:12:15 Overall time: 00:12:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4321357 / 21916100 = 0.1972 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:53:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:53:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:53:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:53:29: 1000000 INFO @ Sat, 15 Jan 2022 18:53:35: 2000000 INFO @ Sat, 15 Jan 2022 18:53:40: 3000000 INFO @ Sat, 15 Jan 2022 18:53:45: 4000000 INFO @ Sat, 15 Jan 2022 18:53:50: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:53:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:53:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:53:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:53:55: 6000000 INFO @ Sat, 15 Jan 2022 18:53:59: 1000000 INFO @ Sat, 15 Jan 2022 18:54:00: 7000000 INFO @ Sat, 15 Jan 2022 18:54:05: 2000000 INFO @ Sat, 15 Jan 2022 18:54:06: 8000000 INFO @ Sat, 15 Jan 2022 18:54:10: 3000000 INFO @ Sat, 15 Jan 2022 18:54:11: 9000000 INFO @ Sat, 15 Jan 2022 18:54:16: 4000000 INFO @ Sat, 15 Jan 2022 18:54:17: 10000000 INFO @ Sat, 15 Jan 2022 18:54:21: 5000000 INFO @ Sat, 15 Jan 2022 18:54:22: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:54:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:54:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:54:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:54:27: 6000000 INFO @ Sat, 15 Jan 2022 18:54:28: 12000000 INFO @ Sat, 15 Jan 2022 18:54:30: 1000000 INFO @ Sat, 15 Jan 2022 18:54:33: 7000000 INFO @ Sat, 15 Jan 2022 18:54:34: 13000000 INFO @ Sat, 15 Jan 2022 18:54:37: 2000000 INFO @ Sat, 15 Jan 2022 18:54:39: 8000000 INFO @ Sat, 15 Jan 2022 18:54:39: 14000000 INFO @ Sat, 15 Jan 2022 18:54:43: 3000000 INFO @ Sat, 15 Jan 2022 18:54:44: 9000000 INFO @ Sat, 15 Jan 2022 18:54:45: 15000000 INFO @ Sat, 15 Jan 2022 18:54:49: 4000000 INFO @ Sat, 15 Jan 2022 18:54:50: 10000000 INFO @ Sat, 15 Jan 2022 18:54:51: 16000000 INFO @ Sat, 15 Jan 2022 18:54:56: 5000000 INFO @ Sat, 15 Jan 2022 18:54:56: 11000000 INFO @ Sat, 15 Jan 2022 18:54:57: 17000000 INFO @ Sat, 15 Jan 2022 18:55:02: 12000000 INFO @ Sat, 15 Jan 2022 18:55:02: 6000000 INFO @ Sat, 15 Jan 2022 18:55:03: 18000000 INFO @ Sat, 15 Jan 2022 18:55:08: 13000000 INFO @ Sat, 15 Jan 2022 18:55:08: 19000000 INFO @ Sat, 15 Jan 2022 18:55:08: 7000000 INFO @ Sat, 15 Jan 2022 18:55:13: 14000000 INFO @ Sat, 15 Jan 2022 18:55:14: 20000000 INFO @ Sat, 15 Jan 2022 18:55:15: 8000000 INFO @ Sat, 15 Jan 2022 18:55:19: 15000000 INFO @ Sat, 15 Jan 2022 18:55:20: 21000000 INFO @ Sat, 15 Jan 2022 18:55:21: 9000000 INFO @ Sat, 15 Jan 2022 18:55:25: 16000000 INFO @ Sat, 15 Jan 2022 18:55:26: 22000000 INFO @ Sat, 15 Jan 2022 18:55:27: 10000000 INFO @ Sat, 15 Jan 2022 18:55:30: 17000000 INFO @ Sat, 15 Jan 2022 18:55:31: 23000000 INFO @ Sat, 15 Jan 2022 18:55:34: 11000000 INFO @ Sat, 15 Jan 2022 18:55:36: 18000000 INFO @ Sat, 15 Jan 2022 18:55:37: 24000000 INFO @ Sat, 15 Jan 2022 18:55:40: 12000000 INFO @ Sat, 15 Jan 2022 18:55:42: 19000000 INFO @ Sat, 15 Jan 2022 18:55:43: 25000000 INFO @ Sat, 15 Jan 2022 18:55:46: 13000000 INFO @ Sat, 15 Jan 2022 18:55:48: 20000000 INFO @ Sat, 15 Jan 2022 18:55:49: 26000000 INFO @ Sat, 15 Jan 2022 18:55:53: 14000000 INFO @ Sat, 15 Jan 2022 18:55:53: 21000000 INFO @ Sat, 15 Jan 2022 18:55:54: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:55:59: 15000000 INFO @ Sat, 15 Jan 2022 18:55:59: 22000000 INFO @ Sat, 15 Jan 2022 18:56:00: 28000000 INFO @ Sat, 15 Jan 2022 18:56:05: 23000000 INFO @ Sat, 15 Jan 2022 18:56:05: 16000000 INFO @ Sat, 15 Jan 2022 18:56:06: 29000000 INFO @ Sat, 15 Jan 2022 18:56:11: 24000000 INFO @ Sat, 15 Jan 2022 18:56:11: 30000000 INFO @ Sat, 15 Jan 2022 18:56:12: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:56:16: 25000000 INFO @ Sat, 15 Jan 2022 18:56:17: 31000000 INFO @ Sat, 15 Jan 2022 18:56:18: 18000000 INFO @ Sat, 15 Jan 2022 18:56:22: 26000000 INFO @ Sat, 15 Jan 2022 18:56:23: 32000000 INFO @ Sat, 15 Jan 2022 18:56:24: 19000000 INFO @ Sat, 15 Jan 2022 18:56:27: 27000000 INFO @ Sat, 15 Jan 2022 18:56:28: 33000000 INFO @ Sat, 15 Jan 2022 18:56:31: 20000000 INFO @ Sat, 15 Jan 2022 18:56:33: 28000000 INFO @ Sat, 15 Jan 2022 18:56:34: 34000000 INFO @ Sat, 15 Jan 2022 18:56:37: 21000000 INFO @ Sat, 15 Jan 2022 18:56:39: 29000000 INFO @ Sat, 15 Jan 2022 18:56:39: 35000000 INFO @ Sat, 15 Jan 2022 18:56:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:56:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:56:43: #1 total tags in treatment: 17530786 INFO @ Sat, 15 Jan 2022 18:56:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:56:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:56:43: #1 tags after filtering in treatment: 9070991 INFO @ Sat, 15 Jan 2022 18:56:43: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 15 Jan 2022 18:56:43: #1 finished! INFO @ Sat, 15 Jan 2022 18:56:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:56:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:56:43: 22000000 INFO @ Sat, 15 Jan 2022 18:56:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:56:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:56:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:56:44: 30000000 INFO @ Sat, 15 Jan 2022 18:56:49: 23000000 INFO @ Sat, 15 Jan 2022 18:56:50: 31000000 INFO @ Sat, 15 Jan 2022 18:56:55: 32000000 INFO @ Sat, 15 Jan 2022 18:56:56: 24000000 INFO @ Sat, 15 Jan 2022 18:57:00: 33000000 INFO @ Sat, 15 Jan 2022 18:57:02: 25000000 INFO @ Sat, 15 Jan 2022 18:57:06: 34000000 INFO @ Sat, 15 Jan 2022 18:57:08: 26000000 INFO @ Sat, 15 Jan 2022 18:57:11: 35000000 INFO @ Sat, 15 Jan 2022 18:57:14: 27000000 INFO @ Sat, 15 Jan 2022 18:57:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:57:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:57:14: #1 total tags in treatment: 17530786 INFO @ Sat, 15 Jan 2022 18:57:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:57:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:57:14: #1 tags after filtering in treatment: 9070991 INFO @ Sat, 15 Jan 2022 18:57:14: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 15 Jan 2022 18:57:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:57:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:57:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:57:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:57:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:57:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:57:19: 28000000 INFO @ Sat, 15 Jan 2022 18:57:25: 29000000 INFO @ Sat, 15 Jan 2022 18:57:31: 30000000 INFO @ Sat, 15 Jan 2022 18:57:37: 31000000 INFO @ Sat, 15 Jan 2022 18:57:42: 32000000 INFO @ Sat, 15 Jan 2022 18:57:48: 33000000 INFO @ Sat, 15 Jan 2022 18:57:53: 34000000 INFO @ Sat, 15 Jan 2022 18:57:59: 35000000 INFO @ Sat, 15 Jan 2022 18:58:02: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:58:02: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:58:02: #1 total tags in treatment: 17530786 INFO @ Sat, 15 Jan 2022 18:58:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:03: #1 tags after filtering in treatment: 9070991 INFO @ Sat, 15 Jan 2022 18:58:03: #1 Redundant rate of treatment: 0.48 INFO @ Sat, 15 Jan 2022 18:58:03: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592366/SRX10592366.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling