Job ID = 14520162 SRX = SRX10592363 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:27:52 prefetch.2.10.7: 1) Downloading 'SRR14227547'... 2022-01-15T09:27:52 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:28:51 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:28:51 prefetch.2.10.7: 1) 'SRR14227547' was downloaded successfully 2022-01-15T09:28:51 prefetch.2.10.7: 'SRR14227547' has 0 unresolved dependencies Read 26572424 spots for SRR14227547/SRR14227547.sra Written 26572424 spots for SRR14227547/SRR14227547.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:25 26572424 reads; of these: 26572424 (100.00%) were paired; of these: 943987 (3.55%) aligned concordantly 0 times 19812083 (74.56%) aligned concordantly exactly 1 time 5816354 (21.89%) aligned concordantly >1 times ---- 943987 pairs aligned concordantly 0 times; of these: 82308 (8.72%) aligned discordantly 1 time ---- 861679 pairs aligned 0 times concordantly or discordantly; of these: 1723358 mates make up the pairs; of these: 1204200 (69.88%) aligned 0 times 388582 (22.55%) aligned exactly 1 time 130576 (7.58%) aligned >1 times 97.73% overall alignment rate Time searching: 00:21:25 Overall time: 00:21:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 9616279 / 25706454 = 0.3741 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:00: 1000000 INFO @ Sat, 15 Jan 2022 19:05:07: 2000000 INFO @ Sat, 15 Jan 2022 19:05:13: 3000000 INFO @ Sat, 15 Jan 2022 19:05:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:26: 5000000 INFO @ Sat, 15 Jan 2022 19:05:29: 1000000 INFO @ Sat, 15 Jan 2022 19:05:33: 6000000 INFO @ Sat, 15 Jan 2022 19:05:36: 2000000 INFO @ Sat, 15 Jan 2022 19:05:40: 7000000 INFO @ Sat, 15 Jan 2022 19:05:43: 3000000 INFO @ Sat, 15 Jan 2022 19:05:47: 8000000 INFO @ Sat, 15 Jan 2022 19:05:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:54: 9000000 INFO @ Sat, 15 Jan 2022 19:05:57: 5000000 INFO @ Sat, 15 Jan 2022 19:05:59: 1000000 INFO @ Sat, 15 Jan 2022 19:06:01: 10000000 INFO @ Sat, 15 Jan 2022 19:06:04: 6000000 INFO @ Sat, 15 Jan 2022 19:06:06: 2000000 INFO @ Sat, 15 Jan 2022 19:06:08: 11000000 INFO @ Sat, 15 Jan 2022 19:06:11: 7000000 INFO @ Sat, 15 Jan 2022 19:06:12: 3000000 INFO @ Sat, 15 Jan 2022 19:06:16: 12000000 INFO @ Sat, 15 Jan 2022 19:06:18: 8000000 INFO @ Sat, 15 Jan 2022 19:06:19: 4000000 INFO @ Sat, 15 Jan 2022 19:06:22: 13000000 INFO @ Sat, 15 Jan 2022 19:06:24: 9000000 INFO @ Sat, 15 Jan 2022 19:06:26: 5000000 INFO @ Sat, 15 Jan 2022 19:06:30: 14000000 INFO @ Sat, 15 Jan 2022 19:06:32: 10000000 INFO @ Sat, 15 Jan 2022 19:06:32: 6000000 INFO @ Sat, 15 Jan 2022 19:06:37: 15000000 INFO @ Sat, 15 Jan 2022 19:06:38: 7000000 INFO @ Sat, 15 Jan 2022 19:06:39: 11000000 INFO @ Sat, 15 Jan 2022 19:06:44: 16000000 INFO @ Sat, 15 Jan 2022 19:06:45: 8000000 INFO @ Sat, 15 Jan 2022 19:06:46: 12000000 INFO @ Sat, 15 Jan 2022 19:06:51: 17000000 INFO @ Sat, 15 Jan 2022 19:06:51: 9000000 INFO @ Sat, 15 Jan 2022 19:06:53: 13000000 INFO @ Sat, 15 Jan 2022 19:06:58: 10000000 INFO @ Sat, 15 Jan 2022 19:06:58: 18000000 INFO @ Sat, 15 Jan 2022 19:07:00: 14000000 INFO @ Sat, 15 Jan 2022 19:07:04: 11000000 INFO @ Sat, 15 Jan 2022 19:07:06: 19000000 INFO @ Sat, 15 Jan 2022 19:07:07: 15000000 INFO @ Sat, 15 Jan 2022 19:07:11: 12000000 INFO @ Sat, 15 Jan 2022 19:07:12: 20000000 INFO @ Sat, 15 Jan 2022 19:07:14: 16000000 INFO @ Sat, 15 Jan 2022 19:07:17: 13000000 INFO @ Sat, 15 Jan 2022 19:07:19: 21000000 INFO @ Sat, 15 Jan 2022 19:07:21: 17000000 INFO @ Sat, 15 Jan 2022 19:07:24: 14000000 INFO @ Sat, 15 Jan 2022 19:07:26: 22000000 INFO @ Sat, 15 Jan 2022 19:07:28: 18000000 INFO @ Sat, 15 Jan 2022 19:07:30: 15000000 INFO @ Sat, 15 Jan 2022 19:07:33: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:07:35: 19000000 INFO @ Sat, 15 Jan 2022 19:07:37: 16000000 INFO @ Sat, 15 Jan 2022 19:07:41: 24000000 INFO @ Sat, 15 Jan 2022 19:07:42: 20000000 INFO @ Sat, 15 Jan 2022 19:07:43: 17000000 INFO @ Sat, 15 Jan 2022 19:07:48: 25000000 INFO @ Sat, 15 Jan 2022 19:07:49: 21000000 INFO @ Sat, 15 Jan 2022 19:07:50: 18000000 INFO @ Sat, 15 Jan 2022 19:07:54: 26000000 INFO @ Sat, 15 Jan 2022 19:07:55: 22000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:07:56: 19000000 INFO @ Sat, 15 Jan 2022 19:08:00: 27000000 INFO @ Sat, 15 Jan 2022 19:08:02: 23000000 INFO @ Sat, 15 Jan 2022 19:08:03: 20000000 INFO @ Sat, 15 Jan 2022 19:08:07: 28000000 INFO @ Sat, 15 Jan 2022 19:08:08: 24000000 INFO @ Sat, 15 Jan 2022 19:08:10: 21000000 INFO @ Sat, 15 Jan 2022 19:08:13: 29000000 INFO @ Sat, 15 Jan 2022 19:08:15: 25000000 INFO @ Sat, 15 Jan 2022 19:08:16: 22000000 INFO @ Sat, 15 Jan 2022 19:08:19: 30000000 INFO @ Sat, 15 Jan 2022 19:08:21: 26000000 INFO @ Sat, 15 Jan 2022 19:08:23: 23000000 INFO @ Sat, 15 Jan 2022 19:08:26: 31000000 INFO @ Sat, 15 Jan 2022 19:08:27: 27000000 INFO @ Sat, 15 Jan 2022 19:08:30: 24000000 INFO @ Sat, 15 Jan 2022 19:08:32: 32000000 INFO @ Sat, 15 Jan 2022 19:08:34: 28000000 INFO @ Sat, 15 Jan 2022 19:08:36: 25000000 INFO @ Sat, 15 Jan 2022 19:08:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:08:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:08:36: #1 total tags in treatment: 16040130 INFO @ Sat, 15 Jan 2022 19:08:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:08:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:08:37: #1 tags after filtering in treatment: 10317080 INFO @ Sat, 15 Jan 2022 19:08:37: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:08:37: #1 finished! INFO @ Sat, 15 Jan 2022 19:08:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:08:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:08:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:08:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:08:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:08:40: 29000000 INFO @ Sat, 15 Jan 2022 19:08:43: 26000000 INFO @ Sat, 15 Jan 2022 19:08:45: 30000000 INFO @ Sat, 15 Jan 2022 19:08:49: 27000000 INFO @ Sat, 15 Jan 2022 19:08:51: 31000000 INFO @ Sat, 15 Jan 2022 19:08:56: 28000000 INFO @ Sat, 15 Jan 2022 19:08:57: 32000000 INFO @ Sat, 15 Jan 2022 19:09:01: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:09:01: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:09:01: #1 total tags in treatment: 16040130 INFO @ Sat, 15 Jan 2022 19:09:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:09:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:09:01: #1 tags after filtering in treatment: 10317080 INFO @ Sat, 15 Jan 2022 19:09:01: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:09:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:09:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:09:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:09:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:09:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:09:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:09:02: 29000000 INFO @ Sat, 15 Jan 2022 19:09:09: 30000000 INFO @ Sat, 15 Jan 2022 19:09:15: 31000000 INFO @ Sat, 15 Jan 2022 19:09:21: 32000000 INFO @ Sat, 15 Jan 2022 19:09:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:09:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:09:26: #1 total tags in treatment: 16040130 INFO @ Sat, 15 Jan 2022 19:09:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:09:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:09:26: #1 tags after filtering in treatment: 10317080 INFO @ Sat, 15 Jan 2022 19:09:26: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:09:26: #1 finished! INFO @ Sat, 15 Jan 2022 19:09:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:09:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:09:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:09:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:09:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592363/SRX10592363.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling