Job ID = 14520160 SRX = SRX10592361 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:27:39 prefetch.2.10.7: 1) Downloading 'SRR14227545'... 2022-01-15T09:27:39 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:28:43 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:28:43 prefetch.2.10.7: 1) 'SRR14227545' was downloaded successfully 2022-01-15T09:28:43 prefetch.2.10.7: 'SRR14227545' has 0 unresolved dependencies Read 25495628 spots for SRR14227545/SRR14227545.sra Written 25495628 spots for SRR14227545/SRR14227545.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:34 25495628 reads; of these: 25495628 (100.00%) were paired; of these: 943864 (3.70%) aligned concordantly 0 times 19145821 (75.09%) aligned concordantly exactly 1 time 5405943 (21.20%) aligned concordantly >1 times ---- 943864 pairs aligned concordantly 0 times; of these: 77484 (8.21%) aligned discordantly 1 time ---- 866380 pairs aligned 0 times concordantly or discordantly; of these: 1732760 mates make up the pairs; of these: 1241957 (71.68%) aligned 0 times 372109 (21.47%) aligned exactly 1 time 118694 (6.85%) aligned >1 times 97.56% overall alignment rate Time searching: 00:23:34 Overall time: 00:23:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 12738818 / 24625420 = 0.5173 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:13:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:13:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:13:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:13:40: 1000000 INFO @ Sat, 15 Jan 2022 19:13:49: 2000000 INFO @ Sat, 15 Jan 2022 19:13:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:14:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:14:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:14:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:14:06: 4000000 INFO @ Sat, 15 Jan 2022 19:14:11: 1000000 INFO @ Sat, 15 Jan 2022 19:14:16: 5000000 INFO @ Sat, 15 Jan 2022 19:14:21: 2000000 INFO @ Sat, 15 Jan 2022 19:14:25: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:14:30: 3000000 INFO @ Sat, 15 Jan 2022 19:14:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:14:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:14:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:14:35: 7000000 INFO @ Sat, 15 Jan 2022 19:14:40: 4000000 INFO @ Sat, 15 Jan 2022 19:14:45: 1000000 INFO @ Sat, 15 Jan 2022 19:14:45: 8000000 INFO @ Sat, 15 Jan 2022 19:14:50: 5000000 INFO @ Sat, 15 Jan 2022 19:14:55: 9000000 INFO @ Sat, 15 Jan 2022 19:14:59: 2000000 INFO @ Sat, 15 Jan 2022 19:14:59: 6000000 INFO @ Sat, 15 Jan 2022 19:15:06: 10000000 INFO @ Sat, 15 Jan 2022 19:15:09: 7000000 INFO @ Sat, 15 Jan 2022 19:15:12: 3000000 INFO @ Sat, 15 Jan 2022 19:15:17: 11000000 INFO @ Sat, 15 Jan 2022 19:15:20: 8000000 INFO @ Sat, 15 Jan 2022 19:15:25: 4000000 INFO @ Sat, 15 Jan 2022 19:15:27: 12000000 INFO @ Sat, 15 Jan 2022 19:15:30: 9000000 INFO @ Sat, 15 Jan 2022 19:15:37: 5000000 INFO @ Sat, 15 Jan 2022 19:15:37: 13000000 INFO @ Sat, 15 Jan 2022 19:15:40: 10000000 INFO @ Sat, 15 Jan 2022 19:15:47: 14000000 INFO @ Sat, 15 Jan 2022 19:15:50: 11000000 INFO @ Sat, 15 Jan 2022 19:15:50: 6000000 INFO @ Sat, 15 Jan 2022 19:15:58: 15000000 INFO @ Sat, 15 Jan 2022 19:15:59: 12000000 INFO @ Sat, 15 Jan 2022 19:16:03: 7000000 INFO @ Sat, 15 Jan 2022 19:16:07: 16000000 INFO @ Sat, 15 Jan 2022 19:16:08: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:16:16: 17000000 INFO @ Sat, 15 Jan 2022 19:16:17: 8000000 INFO @ Sat, 15 Jan 2022 19:16:18: 14000000 INFO @ Sat, 15 Jan 2022 19:16:26: 18000000 INFO @ Sat, 15 Jan 2022 19:16:27: 15000000 INFO @ Sat, 15 Jan 2022 19:16:30: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:16:35: 19000000 INFO @ Sat, 15 Jan 2022 19:16:36: 16000000 INFO @ Sat, 15 Jan 2022 19:16:43: 10000000 INFO @ Sat, 15 Jan 2022 19:16:45: 20000000 INFO @ Sat, 15 Jan 2022 19:16:45: 17000000 INFO @ Sat, 15 Jan 2022 19:16:55: 21000000 INFO @ Sat, 15 Jan 2022 19:16:55: 18000000 INFO @ Sat, 15 Jan 2022 19:16:57: 11000000 INFO @ Sat, 15 Jan 2022 19:17:05: 22000000 INFO @ Sat, 15 Jan 2022 19:17:05: 19000000 INFO @ Sat, 15 Jan 2022 19:17:10: 12000000 INFO @ Sat, 15 Jan 2022 19:17:15: 23000000 INFO @ Sat, 15 Jan 2022 19:17:15: 20000000 INFO @ Sat, 15 Jan 2022 19:17:23: 13000000 INFO @ Sat, 15 Jan 2022 19:17:24: 24000000 INFO @ Sat, 15 Jan 2022 19:17:25: 21000000 INFO @ Sat, 15 Jan 2022 19:17:27: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:17:27: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:17:27: #1 total tags in treatment: 11849673 INFO @ Sat, 15 Jan 2022 19:17:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:17:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:17:27: #1 tags after filtering in treatment: 8013373 INFO @ Sat, 15 Jan 2022 19:17:27: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 19:17:27: #1 finished! INFO @ Sat, 15 Jan 2022 19:17:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:17:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:17:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:17:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:17:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:17:34: 22000000 INFO @ Sat, 15 Jan 2022 19:17:36: 14000000 INFO @ Sat, 15 Jan 2022 19:17:43: 23000000 INFO @ Sat, 15 Jan 2022 19:17:49: 15000000 INFO @ Sat, 15 Jan 2022 19:17:52: 24000000 INFO @ Sat, 15 Jan 2022 19:17:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:17:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:17:54: #1 total tags in treatment: 11849673 INFO @ Sat, 15 Jan 2022 19:17:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:17:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:17:54: #1 tags after filtering in treatment: 8013373 INFO @ Sat, 15 Jan 2022 19:17:54: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 19:17:54: #1 finished! INFO @ Sat, 15 Jan 2022 19:17:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:17:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:17:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:17:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:17:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:18:01: 16000000 INFO @ Sat, 15 Jan 2022 19:18:14: 17000000 INFO @ Sat, 15 Jan 2022 19:18:26: 18000000 INFO @ Sat, 15 Jan 2022 19:18:38: 19000000 INFO @ Sat, 15 Jan 2022 19:18:50: 20000000 INFO @ Sat, 15 Jan 2022 19:19:03: 21000000 INFO @ Sat, 15 Jan 2022 19:19:15: 22000000 INFO @ Sat, 15 Jan 2022 19:19:27: 23000000 INFO @ Sat, 15 Jan 2022 19:19:40: 24000000 INFO @ Sat, 15 Jan 2022 19:19:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:19:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:19:43: #1 total tags in treatment: 11849673 INFO @ Sat, 15 Jan 2022 19:19:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:19:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:19:43: #1 tags after filtering in treatment: 8013373 INFO @ Sat, 15 Jan 2022 19:19:43: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 19:19:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:19:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:19:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:19:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:19:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:19:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592361/SRX10592361.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling