Job ID = 14520159 SRX = SRX10592360 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-01-15T09:27:21 prefetch.2.10.7: 1) Downloading 'SRR14227544'... 2022-01-15T09:27:21 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T09:28:35 prefetch.2.10.7: HTTPS download succeed 2022-01-15T09:28:35 prefetch.2.10.7: 1) 'SRR14227544' was downloaded successfully 2022-01-15T09:28:35 prefetch.2.10.7: 'SRR14227544' has 0 unresolved dependencies Read 21301211 spots for SRR14227544/SRR14227544.sra Written 21301211 spots for SRR14227544/SRR14227544.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:03 21301211 reads; of these: 21301211 (100.00%) were paired; of these: 742778 (3.49%) aligned concordantly 0 times 16398756 (76.99%) aligned concordantly exactly 1 time 4159677 (19.53%) aligned concordantly >1 times ---- 742778 pairs aligned concordantly 0 times; of these: 85227 (11.47%) aligned discordantly 1 time ---- 657551 pairs aligned 0 times concordantly or discordantly; of these: 1315102 mates make up the pairs; of these: 871957 (66.30%) aligned 0 times 338982 (25.78%) aligned exactly 1 time 104163 (7.92%) aligned >1 times 97.95% overall alignment rate Time searching: 00:12:03 Overall time: 00:12:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 8154214 / 20640051 = 0.3951 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:50:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:50:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:50:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:50:39: 1000000 INFO @ Sat, 15 Jan 2022 18:50:44: 2000000 INFO @ Sat, 15 Jan 2022 18:50:48: 3000000 INFO @ Sat, 15 Jan 2022 18:50:52: 4000000 INFO @ Sat, 15 Jan 2022 18:50:57: 5000000 INFO @ Sat, 15 Jan 2022 18:51:01: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:51:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:51:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:51:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:51:06: 7000000 INFO @ Sat, 15 Jan 2022 18:51:10: 1000000 INFO @ Sat, 15 Jan 2022 18:51:11: 8000000 INFO @ Sat, 15 Jan 2022 18:51:14: 2000000 INFO @ Sat, 15 Jan 2022 18:51:16: 9000000 INFO @ Sat, 15 Jan 2022 18:51:19: 3000000 INFO @ Sat, 15 Jan 2022 18:51:20: 10000000 INFO @ Sat, 15 Jan 2022 18:51:24: 4000000 INFO @ Sat, 15 Jan 2022 18:51:25: 11000000 INFO @ Sat, 15 Jan 2022 18:51:29: 5000000 INFO @ Sat, 15 Jan 2022 18:51:30: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:51:34: 6000000 INFO @ Sat, 15 Jan 2022 18:51:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:51:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:51:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:51:35: 13000000 INFO @ Sat, 15 Jan 2022 18:51:39: 7000000 INFO @ Sat, 15 Jan 2022 18:51:40: 1000000 INFO @ Sat, 15 Jan 2022 18:51:40: 14000000 INFO @ Sat, 15 Jan 2022 18:51:44: 8000000 INFO @ Sat, 15 Jan 2022 18:51:45: 2000000 INFO @ Sat, 15 Jan 2022 18:51:45: 15000000 INFO @ Sat, 15 Jan 2022 18:51:49: 9000000 INFO @ Sat, 15 Jan 2022 18:51:49: 3000000 INFO @ Sat, 15 Jan 2022 18:51:50: 16000000 INFO @ Sat, 15 Jan 2022 18:51:54: 10000000 INFO @ Sat, 15 Jan 2022 18:51:54: 4000000 INFO @ Sat, 15 Jan 2022 18:51:55: 17000000 INFO @ Sat, 15 Jan 2022 18:51:59: 11000000 INFO @ Sat, 15 Jan 2022 18:51:59: 5000000 INFO @ Sat, 15 Jan 2022 18:52:00: 18000000 INFO @ Sat, 15 Jan 2022 18:52:04: 12000000 INFO @ Sat, 15 Jan 2022 18:52:04: 6000000 INFO @ Sat, 15 Jan 2022 18:52:04: 19000000 INFO @ Sat, 15 Jan 2022 18:52:09: 13000000 INFO @ Sat, 15 Jan 2022 18:52:09: 7000000 INFO @ Sat, 15 Jan 2022 18:52:09: 20000000 INFO @ Sat, 15 Jan 2022 18:52:13: 14000000 INFO @ Sat, 15 Jan 2022 18:52:14: 8000000 INFO @ Sat, 15 Jan 2022 18:52:14: 21000000 INFO @ Sat, 15 Jan 2022 18:52:19: 15000000 INFO @ Sat, 15 Jan 2022 18:52:19: 9000000 INFO @ Sat, 15 Jan 2022 18:52:19: 22000000 INFO @ Sat, 15 Jan 2022 18:52:24: 10000000 INFO @ Sat, 15 Jan 2022 18:52:24: 23000000 INFO @ Sat, 15 Jan 2022 18:52:24: 16000000 INFO @ Sat, 15 Jan 2022 18:52:29: 17000000 INFO @ Sat, 15 Jan 2022 18:52:29: 24000000 INFO @ Sat, 15 Jan 2022 18:52:29: 11000000 INFO @ Sat, 15 Jan 2022 18:52:34: 18000000 INFO @ Sat, 15 Jan 2022 18:52:34: 25000000 INFO @ Sat, 15 Jan 2022 18:52:34: 12000000 INFO @ Sat, 15 Jan 2022 18:52:36: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:52:36: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:52:36: #1 total tags in treatment: 12435696 INFO @ Sat, 15 Jan 2022 18:52:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:52:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:52:36: #1 tags after filtering in treatment: 8513569 INFO @ Sat, 15 Jan 2022 18:52:36: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 18:52:36: #1 finished! INFO @ Sat, 15 Jan 2022 18:52:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:52:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:52:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:52:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:52:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:52:39: 19000000 INFO @ Sat, 15 Jan 2022 18:52:39: 13000000 INFO @ Sat, 15 Jan 2022 18:52:44: 20000000 INFO @ Sat, 15 Jan 2022 18:52:44: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:52:48: 21000000 INFO @ Sat, 15 Jan 2022 18:52:49: 15000000 INFO @ Sat, 15 Jan 2022 18:52:54: 16000000 INFO @ Sat, 15 Jan 2022 18:52:54: 22000000 INFO @ Sat, 15 Jan 2022 18:52:59: 17000000 INFO @ Sat, 15 Jan 2022 18:52:59: 23000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:53:05: 18000000 INFO @ Sat, 15 Jan 2022 18:53:05: 24000000 INFO @ Sat, 15 Jan 2022 18:53:10: 19000000 INFO @ Sat, 15 Jan 2022 18:53:11: 25000000 INFO @ Sat, 15 Jan 2022 18:53:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:53:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:53:13: #1 total tags in treatment: 12435696 INFO @ Sat, 15 Jan 2022 18:53:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:53:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:53:14: #1 tags after filtering in treatment: 8513569 INFO @ Sat, 15 Jan 2022 18:53:14: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 18:53:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:53:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:53:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:53:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:53:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:53:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:53:15: 20000000 INFO @ Sat, 15 Jan 2022 18:53:21: 21000000 INFO @ Sat, 15 Jan 2022 18:53:25: 22000000 INFO @ Sat, 15 Jan 2022 18:53:31: 23000000 INFO @ Sat, 15 Jan 2022 18:53:36: 24000000 INFO @ Sat, 15 Jan 2022 18:53:41: 25000000 INFO @ Sat, 15 Jan 2022 18:53:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:53:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:53:43: #1 total tags in treatment: 12435696 INFO @ Sat, 15 Jan 2022 18:53:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:53:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:53:44: #1 tags after filtering in treatment: 8513569 INFO @ Sat, 15 Jan 2022 18:53:44: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 18:53:44: #1 finished! INFO @ Sat, 15 Jan 2022 18:53:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:53:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:53:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:53:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:53:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10592360/SRX10592360.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling