Job ID = 14520680 SRX = SRX10583013 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11131375 spots for SRR14217989/SRR14217989.sra Written 11131375 spots for SRR14217989/SRR14217989.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:16 11131375 reads; of these: 11131375 (100.00%) were unpaired; of these: 4745296 (42.63%) aligned 0 times 5634491 (50.62%) aligned exactly 1 time 751588 (6.75%) aligned >1 times 57.37% overall alignment rate Time searching: 00:01:16 Overall time: 00:01:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1762239 / 6386079 = 0.2760 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:40:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:12: 1000000 INFO @ Sat, 15 Jan 2022 19:40:18: 2000000 INFO @ Sat, 15 Jan 2022 19:40:24: 3000000 INFO @ Sat, 15 Jan 2022 19:40:30: 4000000 INFO @ Sat, 15 Jan 2022 19:40:33: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:40:33: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:40:33: #1 total tags in treatment: 4623840 INFO @ Sat, 15 Jan 2022 19:40:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:33: #1 tags after filtering in treatment: 4623840 INFO @ Sat, 15 Jan 2022 19:40:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:40:33: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:34: #2 number of paired peaks: 19 WARNING @ Sat, 15 Jan 2022 19:40:34: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:40:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:43: 1000000 INFO @ Sat, 15 Jan 2022 19:40:49: 2000000 INFO @ Sat, 15 Jan 2022 19:40:56: 3000000 INFO @ Sat, 15 Jan 2022 19:41:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:41:05: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:41:05: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:41:05: #1 total tags in treatment: 4623840 INFO @ Sat, 15 Jan 2022 19:41:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:41:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:41:06: #1 tags after filtering in treatment: 4623840 INFO @ Sat, 15 Jan 2022 19:41:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:41:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:41:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:41:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:41:06: #2 number of paired peaks: 19 WARNING @ Sat, 15 Jan 2022 19:41:06: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:41:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:41:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:41:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:41:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:41:12: 1000000 INFO @ Sat, 15 Jan 2022 19:41:18: 2000000 INFO @ Sat, 15 Jan 2022 19:41:24: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:41:30: 4000000 INFO @ Sat, 15 Jan 2022 19:41:33: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:41:33: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:41:33: #1 total tags in treatment: 4623840 INFO @ Sat, 15 Jan 2022 19:41:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:41:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:41:33: #1 tags after filtering in treatment: 4623840 INFO @ Sat, 15 Jan 2022 19:41:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:41:33: #1 finished! INFO @ Sat, 15 Jan 2022 19:41:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:41:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:41:34: #2 number of paired peaks: 19 WARNING @ Sat, 15 Jan 2022 19:41:34: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:41:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583013/SRX10583013.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。