Job ID = 14520667 SRX = SRX10583006 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10673476 spots for SRR14217973/SRR14217973.sra Written 10673476 spots for SRR14217973/SRR14217973.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:47 10673476 reads; of these: 10673476 (100.00%) were unpaired; of these: 1146173 (10.74%) aligned 0 times 8520824 (79.83%) aligned exactly 1 time 1006479 (9.43%) aligned >1 times 89.26% overall alignment rate Time searching: 00:01:47 Overall time: 00:01:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3089152 / 9527303 = 0.3242 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:39:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:39:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:39:33: 1000000 INFO @ Sat, 15 Jan 2022 19:39:39: 2000000 INFO @ Sat, 15 Jan 2022 19:39:46: 3000000 INFO @ Sat, 15 Jan 2022 19:39:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:39:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:39:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:00: 5000000 INFO @ Sat, 15 Jan 2022 19:40:04: 1000000 INFO @ Sat, 15 Jan 2022 19:40:07: 6000000 INFO @ Sat, 15 Jan 2022 19:40:10: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:40:10: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:40:10: #1 total tags in treatment: 6438151 INFO @ Sat, 15 Jan 2022 19:40:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:10: #1 tags after filtering in treatment: 6438151 INFO @ Sat, 15 Jan 2022 19:40:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:40:10: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:40:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:40:13: 2000000 INFO @ Sat, 15 Jan 2022 19:40:21: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:40:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:29: 4000000 INFO @ Sat, 15 Jan 2022 19:40:33: 1000000 INFO @ Sat, 15 Jan 2022 19:40:37: 5000000 INFO @ Sat, 15 Jan 2022 19:40:40: 2000000 INFO @ Sat, 15 Jan 2022 19:40:46: 6000000 INFO @ Sat, 15 Jan 2022 19:40:47: 3000000 INFO @ Sat, 15 Jan 2022 19:40:49: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:40:49: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:40:49: #1 total tags in treatment: 6438151 INFO @ Sat, 15 Jan 2022 19:40:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:49: #1 tags after filtering in treatment: 6438151 INFO @ Sat, 15 Jan 2022 19:40:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:40:49: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:40:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:40:54: 4000000 INFO @ Sat, 15 Jan 2022 19:41:00: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:41:08: 6000000 INFO @ Sat, 15 Jan 2022 19:41:10: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:41:10: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:41:10: #1 total tags in treatment: 6438151 INFO @ Sat, 15 Jan 2022 19:41:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:41:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:41:11: #1 tags after filtering in treatment: 6438151 INFO @ Sat, 15 Jan 2022 19:41:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:41:11: #1 finished! INFO @ Sat, 15 Jan 2022 19:41:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:41:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:41:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:41:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:41:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583006/SRX10583006.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling