Job ID = 14520665 SRX = SRX10583004 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9472577 spots for SRR14217969/SRR14217969.sra Written 9472577 spots for SRR14217969/SRR14217969.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:50 9472577 reads; of these: 9472577 (100.00%) were unpaired; of these: 834916 (8.81%) aligned 0 times 7701988 (81.31%) aligned exactly 1 time 935673 (9.88%) aligned >1 times 91.19% overall alignment rate Time searching: 00:01:50 Overall time: 00:01:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2807027 / 8637661 = 0.3250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:39:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:39:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:39:51: 1000000 INFO @ Sat, 15 Jan 2022 19:39:59: 2000000 INFO @ Sat, 15 Jan 2022 19:40:07: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:40:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:16: 4000000 INFO @ Sat, 15 Jan 2022 19:40:21: 1000000 INFO @ Sat, 15 Jan 2022 19:40:24: 5000000 INFO @ Sat, 15 Jan 2022 19:40:30: 2000000 INFO @ Sat, 15 Jan 2022 19:40:31: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:40:31: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:40:31: #1 total tags in treatment: 5830634 INFO @ Sat, 15 Jan 2022 19:40:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:31: #1 tags after filtering in treatment: 5830634 INFO @ Sat, 15 Jan 2022 19:40:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:40:31: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:40:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:40:39: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:40:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:47: 4000000 INFO @ Sat, 15 Jan 2022 19:40:53: 1000000 INFO @ Sat, 15 Jan 2022 19:40:56: 5000000 INFO @ Sat, 15 Jan 2022 19:41:03: 2000000 INFO @ Sat, 15 Jan 2022 19:41:05: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:41:05: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:41:05: #1 total tags in treatment: 5830634 INFO @ Sat, 15 Jan 2022 19:41:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:41:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:41:05: #1 tags after filtering in treatment: 5830634 INFO @ Sat, 15 Jan 2022 19:41:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:41:05: #1 finished! INFO @ Sat, 15 Jan 2022 19:41:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:41:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:41:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:41:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:41:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:41:14: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:41:23: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:41:32: 5000000 INFO @ Sat, 15 Jan 2022 19:41:40: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 19:41:40: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 19:41:40: #1 total tags in treatment: 5830634 INFO @ Sat, 15 Jan 2022 19:41:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:41:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:41:40: #1 tags after filtering in treatment: 5830634 INFO @ Sat, 15 Jan 2022 19:41:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:41:40: #1 finished! INFO @ Sat, 15 Jan 2022 19:41:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:41:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:41:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:41:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:41:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10583004/SRX10583004.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling