Job ID = 14521003 SRX = SRX10574732 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-01-15T11:14:49 prefetch.2.10.7: 1) Downloading 'SRR14208001'... 2022-01-15T11:14:49 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T11:14:52 prefetch.2.10.7: HTTPS download succeed 2022-01-15T11:14:52 prefetch.2.10.7: 'SRR14208001' is valid 2022-01-15T11:14:52 prefetch.2.10.7: 1) 'SRR14208001' was downloaded successfully 2022-01-15T11:14:52 prefetch.2.10.7: 'SRR14208001' has 0 unresolved dependencies Read 802073 spots for SRR14208001/SRR14208001.sra Written 802073 spots for SRR14208001/SRR14208001.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:06 802073 reads; of these: 802073 (100.00%) were unpaired; of these: 65771 (8.20%) aligned 0 times 585465 (72.99%) aligned exactly 1 time 150837 (18.81%) aligned >1 times 91.80% overall alignment rate Time searching: 00:00:06 Overall time: 00:00:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 84627 / 736302 = 0.1149 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:51: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:15:51: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:15:51: #1 total tags in treatment: 651675 INFO @ Sat, 15 Jan 2022 20:15:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:15:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:15:51: #1 tags after filtering in treatment: 651675 INFO @ Sat, 15 Jan 2022 20:15:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:15:51: #1 finished! INFO @ Sat, 15 Jan 2022 20:15:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:15:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:15:51: #2 number of paired peaks: 49 WARNING @ Sat, 15 Jan 2022 20:15:51: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:15:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:21: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:16:21: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:16:21: #1 total tags in treatment: 651675 INFO @ Sat, 15 Jan 2022 20:16:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:21: #1 tags after filtering in treatment: 651675 INFO @ Sat, 15 Jan 2022 20:16:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:16:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:21: #2 number of paired peaks: 49 WARNING @ Sat, 15 Jan 2022 20:16:21: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:47: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:16:51: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:16:51: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:16:51: #1 total tags in treatment: 651675 INFO @ Sat, 15 Jan 2022 20:16:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:51: #1 tags after filtering in treatment: 651675 INFO @ Sat, 15 Jan 2022 20:16:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:16:51: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:51: #2 number of paired peaks: 49 WARNING @ Sat, 15 Jan 2022 20:16:51: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574732/SRX10574732.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling