Job ID = 14521001 SRX = SRX10574731 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-01-15T11:14:48 prefetch.2.10.7: 1) Downloading 'SRR14208000'... 2022-01-15T11:14:48 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T11:14:51 prefetch.2.10.7: HTTPS download succeed 2022-01-15T11:14:51 prefetch.2.10.7: 'SRR14208000' is valid 2022-01-15T11:14:51 prefetch.2.10.7: 1) 'SRR14208000' was downloaded successfully 2022-01-15T11:14:51 prefetch.2.10.7: 'SRR14208000' has 0 unresolved dependencies Read 247036 spots for SRR14208000/SRR14208000.sra Written 247036 spots for SRR14208000/SRR14208000.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:02 247036 reads; of these: 247036 (100.00%) were unpaired; of these: 53854 (21.80%) aligned 0 times 150443 (60.90%) aligned exactly 1 time 42739 (17.30%) aligned >1 times 78.20% overall alignment rate Time searching: 00:00:02 Overall time: 00:00:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 12015 / 193182 = 0.0622 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:30: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:15:30: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:15:30: #1 total tags in treatment: 181167 INFO @ Sat, 15 Jan 2022 20:15:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:15:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:15:30: #1 tags after filtering in treatment: 181167 INFO @ Sat, 15 Jan 2022 20:15:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:15:30: #1 finished! INFO @ Sat, 15 Jan 2022 20:15:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:15:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:15:30: #2 number of paired peaks: 3 WARNING @ Sat, 15 Jan 2022 20:15:30: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:15:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:00: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:16:00: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:16:00: #1 total tags in treatment: 181167 INFO @ Sat, 15 Jan 2022 20:16:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:00: #1 tags after filtering in treatment: 181167 INFO @ Sat, 15 Jan 2022 20:16:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:16:00: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:00: #2 number of paired peaks: 3 WARNING @ Sat, 15 Jan 2022 20:16:00: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:16:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:30: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:16:30: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:16:30: #1 total tags in treatment: 181167 INFO @ Sat, 15 Jan 2022 20:16:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:30: #1 tags after filtering in treatment: 181167 INFO @ Sat, 15 Jan 2022 20:16:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:16:30: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:30: #2 number of paired peaks: 3 WARNING @ Sat, 15 Jan 2022 20:16:30: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574731/SRX10574731.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling