Job ID = 14521000 SRX = SRX10574730 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-01-15T11:14:34 prefetch.2.10.7: 1) Downloading 'SRR14207999'... 2022-01-15T11:14:34 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T11:14:37 prefetch.2.10.7: HTTPS download succeed 2022-01-15T11:14:37 prefetch.2.10.7: 'SRR14207999' is valid 2022-01-15T11:14:37 prefetch.2.10.7: 1) 'SRR14207999' was downloaded successfully 2022-01-15T11:14:37 prefetch.2.10.7: 'SRR14207999' has 0 unresolved dependencies Read 509744 spots for SRR14207999/SRR14207999.sra Written 509744 spots for SRR14207999/SRR14207999.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:04 509744 reads; of these: 509744 (100.00%) were unpaired; of these: 40097 (7.87%) aligned 0 times 359564 (70.54%) aligned exactly 1 time 110083 (21.60%) aligned >1 times 92.13% overall alignment rate Time searching: 00:00:04 Overall time: 00:00:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 59254 / 469647 = 0.1262 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:26: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:15:26: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:15:26: #1 total tags in treatment: 410393 INFO @ Sat, 15 Jan 2022 20:15:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:15:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:15:26: #1 tags after filtering in treatment: 410393 INFO @ Sat, 15 Jan 2022 20:15:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:15:26: #1 finished! INFO @ Sat, 15 Jan 2022 20:15:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:15:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:15:26: #2 number of paired peaks: 34 WARNING @ Sat, 15 Jan 2022 20:15:26: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:15:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:56: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:15:56: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:15:56: #1 total tags in treatment: 410393 INFO @ Sat, 15 Jan 2022 20:15:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:15:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:15:56: #1 tags after filtering in treatment: 410393 INFO @ Sat, 15 Jan 2022 20:15:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:15:56: #1 finished! INFO @ Sat, 15 Jan 2022 20:15:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:15:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:15:56: #2 number of paired peaks: 34 WARNING @ Sat, 15 Jan 2022 20:15:56: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:15:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:16:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:24: #1 read treatment tags... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:16:26: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:16:26: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:16:26: #1 total tags in treatment: 410393 INFO @ Sat, 15 Jan 2022 20:16:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:26: #1 tags after filtering in treatment: 410393 INFO @ Sat, 15 Jan 2022 20:16:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:16:26: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:26: #2 number of paired peaks: 34 WARNING @ Sat, 15 Jan 2022 20:16:26: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574730/SRX10574730.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling