Job ID = 14520943 SRX = SRX10574729 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-01-15T11:09:11 prefetch.2.10.7: 1) Downloading 'SRR14207998'... 2022-01-15T11:09:11 prefetch.2.10.7: Downloading via HTTPS... 2022-01-15T11:09:14 prefetch.2.10.7: HTTPS download succeed 2022-01-15T11:09:14 prefetch.2.10.7: 'SRR14207998' is valid 2022-01-15T11:09:14 prefetch.2.10.7: 1) 'SRR14207998' was downloaded successfully 2022-01-15T11:09:14 prefetch.2.10.7: 'SRR14207998' has 0 unresolved dependencies Read 283736 spots for SRR14207998/SRR14207998.sra Written 283736 spots for SRR14207998/SRR14207998.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:02 283736 reads; of these: 283736 (100.00%) were unpaired; of these: 70779 (24.95%) aligned 0 times 158082 (55.71%) aligned exactly 1 time 54875 (19.34%) aligned >1 times 75.05% overall alignment rate Time searching: 00:00:02 Overall time: 00:00:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 19497 / 212957 = 0.0916 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:53: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:09:53: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:09:53: #1 total tags in treatment: 193460 INFO @ Sat, 15 Jan 2022 20:09:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:53: #1 tags after filtering in treatment: 193460 INFO @ Sat, 15 Jan 2022 20:09:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:09:53: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:53: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 20:09:53: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:10:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:10:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:10:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:10:23: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:10:23: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:10:23: #1 total tags in treatment: 193460 INFO @ Sat, 15 Jan 2022 20:10:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:10:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:10:23: #1 tags after filtering in treatment: 193460 INFO @ Sat, 15 Jan 2022 20:10:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:10:23: #1 finished! INFO @ Sat, 15 Jan 2022 20:10:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:10:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:10:23: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 20:10:23: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:10:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:10:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:10:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:10:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:10:53: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:10:53: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:10:53: #1 total tags in treatment: 193460 INFO @ Sat, 15 Jan 2022 20:10:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:10:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:10:53: #1 tags after filtering in treatment: 193460 INFO @ Sat, 15 Jan 2022 20:10:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:10:53: #1 finished! INFO @ Sat, 15 Jan 2022 20:10:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:10:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:10:53: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 20:10:53: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:10:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10574729/SRX10574729.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling