Job ID = 2009699 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,550,655 reads read : 17,550,655 reads written : 17,550,655 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR364351.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:33 17550655 reads; of these: 17550655 (100.00%) were unpaired; of these: 1705648 (9.72%) aligned 0 times 12747202 (72.63%) aligned exactly 1 time 3097805 (17.65%) aligned >1 times 90.28% overall alignment rate Time searching: 00:02:33 Overall time: 00:02:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 6209015 / 15845007 = 0.3919 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:36:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:36:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:36:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:36:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:36:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:36:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:36:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:36:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:36:22: 1000000 INFO @ Fri, 05 Jul 2019 19:36:27: 1000000 INFO @ Fri, 05 Jul 2019 19:36:28: 1000000 INFO @ Fri, 05 Jul 2019 19:36:28: 2000000 INFO @ Fri, 05 Jul 2019 19:36:33: 2000000 INFO @ Fri, 05 Jul 2019 19:36:35: 3000000 INFO @ Fri, 05 Jul 2019 19:36:36: 2000000 INFO @ Fri, 05 Jul 2019 19:36:39: 3000000 INFO @ Fri, 05 Jul 2019 19:36:41: 4000000 INFO @ Fri, 05 Jul 2019 19:36:44: 3000000 INFO @ Fri, 05 Jul 2019 19:36:46: 4000000 INFO @ Fri, 05 Jul 2019 19:36:47: 5000000 INFO @ Fri, 05 Jul 2019 19:36:52: 4000000 INFO @ Fri, 05 Jul 2019 19:36:52: 5000000 INFO @ Fri, 05 Jul 2019 19:36:54: 6000000 INFO @ Fri, 05 Jul 2019 19:36:59: 6000000 INFO @ Fri, 05 Jul 2019 19:37:00: 5000000 INFO @ Fri, 05 Jul 2019 19:37:00: 7000000 INFO @ Fri, 05 Jul 2019 19:37:05: 7000000 INFO @ Fri, 05 Jul 2019 19:37:07: 8000000 INFO @ Fri, 05 Jul 2019 19:37:08: 6000000 INFO @ Fri, 05 Jul 2019 19:37:12: 8000000 INFO @ Fri, 05 Jul 2019 19:37:17: 9000000 INFO @ Fri, 05 Jul 2019 19:37:18: 9000000 INFO @ Fri, 05 Jul 2019 19:37:18: 7000000 INFO @ Fri, 05 Jul 2019 19:37:21: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:37:21: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:37:21: #1 total tags in treatment: 9635992 INFO @ Fri, 05 Jul 2019 19:37:21: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:21: #1 tags after filtering in treatment: 9635992 INFO @ Fri, 05 Jul 2019 19:37:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:21: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:22: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:37:22: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:37:22: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:37:22: #1 total tags in treatment: 9635992 INFO @ Fri, 05 Jul 2019 19:37:22: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:22: #1 tags after filtering in treatment: 9635992 INFO @ Fri, 05 Jul 2019 19:37:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:22: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:22: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:23: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:37:26: 8000000 INFO @ Fri, 05 Jul 2019 19:37:34: 9000000 INFO @ Fri, 05 Jul 2019 19:37:39: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:37:39: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:37:39: #1 total tags in treatment: 9635992 INFO @ Fri, 05 Jul 2019 19:37:39: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:39: #1 tags after filtering in treatment: 9635992 INFO @ Fri, 05 Jul 2019 19:37:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:39: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:39: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:40: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105565/SRX105565.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。