Job ID = 2009692 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,910,779 reads read : 19,910,779 reads written : 19,910,779 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR364347.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:49 19910779 reads; of these: 19910779 (100.00%) were unpaired; of these: 1764249 (8.86%) aligned 0 times 15441133 (77.55%) aligned exactly 1 time 2705397 (13.59%) aligned >1 times 91.14% overall alignment rate Time searching: 00:02:49 Overall time: 00:02:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7806172 / 18146530 = 0.4302 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:35:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:35:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:35:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:35:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:35:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:35:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:35:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:35:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:35:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:35:41: 1000000 INFO @ Fri, 05 Jul 2019 19:35:41: 1000000 INFO @ Fri, 05 Jul 2019 19:35:43: 1000000 INFO @ Fri, 05 Jul 2019 19:35:50: 2000000 INFO @ Fri, 05 Jul 2019 19:35:50: 2000000 INFO @ Fri, 05 Jul 2019 19:35:52: 2000000 INFO @ Fri, 05 Jul 2019 19:35:58: 3000000 INFO @ Fri, 05 Jul 2019 19:36:00: 3000000 INFO @ Fri, 05 Jul 2019 19:36:00: 3000000 INFO @ Fri, 05 Jul 2019 19:36:07: 4000000 INFO @ Fri, 05 Jul 2019 19:36:08: 4000000 INFO @ Fri, 05 Jul 2019 19:36:10: 4000000 INFO @ Fri, 05 Jul 2019 19:36:16: 5000000 INFO @ Fri, 05 Jul 2019 19:36:16: 5000000 INFO @ Fri, 05 Jul 2019 19:36:19: 5000000 INFO @ Fri, 05 Jul 2019 19:36:23: 6000000 INFO @ Fri, 05 Jul 2019 19:36:25: 6000000 INFO @ Fri, 05 Jul 2019 19:36:28: 6000000 INFO @ Fri, 05 Jul 2019 19:36:31: 7000000 INFO @ Fri, 05 Jul 2019 19:36:33: 7000000 INFO @ Fri, 05 Jul 2019 19:36:37: 7000000 INFO @ Fri, 05 Jul 2019 19:36:39: 8000000 INFO @ Fri, 05 Jul 2019 19:36:42: 8000000 INFO @ Fri, 05 Jul 2019 19:36:46: 8000000 INFO @ Fri, 05 Jul 2019 19:36:46: 9000000 INFO @ Fri, 05 Jul 2019 19:36:50: 9000000 INFO @ Fri, 05 Jul 2019 19:36:54: 10000000 INFO @ Fri, 05 Jul 2019 19:36:56: 9000000 INFO @ Fri, 05 Jul 2019 19:36:56: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:36:56: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:36:56: #1 total tags in treatment: 10340358 INFO @ Fri, 05 Jul 2019 19:36:56: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:36:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:36:57: #1 tags after filtering in treatment: 10340358 INFO @ Fri, 05 Jul 2019 19:36:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:36:57: #1 finished! INFO @ Fri, 05 Jul 2019 19:36:57: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:36:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:36:57: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:36:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:36:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:36:59: 10000000 INFO @ Fri, 05 Jul 2019 19:37:02: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:37:02: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:37:02: #1 total tags in treatment: 10340358 INFO @ Fri, 05 Jul 2019 19:37:02: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:02: #1 tags after filtering in treatment: 10340358 INFO @ Fri, 05 Jul 2019 19:37:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:02: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:02: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:03: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:03: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:37:06: 10000000 INFO @ Fri, 05 Jul 2019 19:37:09: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:37:09: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:37:09: #1 total tags in treatment: 10340358 INFO @ Fri, 05 Jul 2019 19:37:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:09: #1 tags after filtering in treatment: 10340358 INFO @ Fri, 05 Jul 2019 19:37:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:09: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:10: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105561/SRX105561.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。