Job ID = 14521350 SRX = SRX10468556 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4403607 spots for SRR14094866/SRR14094866.sra Written 4403607 spots for SRR14094866/SRR14094866.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:14 4403607 reads; of these: 4403607 (100.00%) were paired; of these: 1387116 (31.50%) aligned concordantly 0 times 2478361 (56.28%) aligned concordantly exactly 1 time 538130 (12.22%) aligned concordantly >1 times ---- 1387116 pairs aligned concordantly 0 times; of these: 10607 (0.76%) aligned discordantly 1 time ---- 1376509 pairs aligned 0 times concordantly or discordantly; of these: 2753018 mates make up the pairs; of these: 2659115 (96.59%) aligned 0 times 67370 (2.45%) aligned exactly 1 time 26533 (0.96%) aligned >1 times 69.81% overall alignment rate Time searching: 00:01:14 Overall time: 00:01:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 202038 / 3017405 = 0.0670 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:54:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:54:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:54:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:54:58: 1000000 INFO @ Sat, 15 Jan 2022 20:55:03: 2000000 INFO @ Sat, 15 Jan 2022 20:55:07: 3000000 INFO @ Sat, 15 Jan 2022 20:55:11: 4000000 INFO @ Sat, 15 Jan 2022 20:55:15: 5000000 INFO @ Sat, 15 Jan 2022 20:55:19: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 20:55:19: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 20:55:19: #1 total tags in treatment: 2814499 INFO @ Sat, 15 Jan 2022 20:55:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:55:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:55:19: #1 tags after filtering in treatment: 1884184 INFO @ Sat, 15 Jan 2022 20:55:19: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 20:55:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:55:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:55:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:55:19: #2 number of paired peaks: 154 WARNING @ Sat, 15 Jan 2022 20:55:19: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Sat, 15 Jan 2022 20:55:19: start model_add_line... INFO @ Sat, 15 Jan 2022 20:55:19: start X-correlation... INFO @ Sat, 15 Jan 2022 20:55:19: end of X-cor INFO @ Sat, 15 Jan 2022 20:55:19: #2 finished! INFO @ Sat, 15 Jan 2022 20:55:19: #2 predicted fragment length is 149 bps INFO @ Sat, 15 Jan 2022 20:55:19: #2 alternative fragment length(s) may be 149 bps INFO @ Sat, 15 Jan 2022 20:55:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.05_model.r INFO @ Sat, 15 Jan 2022 20:55:19: #3 Call peaks... INFO @ Sat, 15 Jan 2022 20:55:19: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:55:23: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 20:55:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:55:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:55:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:55:25: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.05_peaks.xls INFO @ Sat, 15 Jan 2022 20:55:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.05_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 20:55:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.05_summits.bed INFO @ Sat, 15 Jan 2022 20:55:25: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (3273 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:55:28: 1000000 INFO @ Sat, 15 Jan 2022 20:55:33: 2000000 INFO @ Sat, 15 Jan 2022 20:55:37: 3000000 INFO @ Sat, 15 Jan 2022 20:55:41: 4000000 INFO @ Sat, 15 Jan 2022 20:55:45: 5000000 INFO @ Sat, 15 Jan 2022 20:55:49: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 20:55:49: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 20:55:49: #1 total tags in treatment: 2814499 INFO @ Sat, 15 Jan 2022 20:55:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:55:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:55:49: #1 tags after filtering in treatment: 1884184 INFO @ Sat, 15 Jan 2022 20:55:49: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 20:55:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:55:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:55:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:55:49: #2 number of paired peaks: 154 WARNING @ Sat, 15 Jan 2022 20:55:49: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Sat, 15 Jan 2022 20:55:49: start model_add_line... INFO @ Sat, 15 Jan 2022 20:55:49: start X-correlation... INFO @ Sat, 15 Jan 2022 20:55:49: end of X-cor INFO @ Sat, 15 Jan 2022 20:55:49: #2 finished! INFO @ Sat, 15 Jan 2022 20:55:49: #2 predicted fragment length is 149 bps INFO @ Sat, 15 Jan 2022 20:55:49: #2 alternative fragment length(s) may be 149 bps INFO @ Sat, 15 Jan 2022 20:55:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.10_model.r INFO @ Sat, 15 Jan 2022 20:55:49: #3 Call peaks... INFO @ Sat, 15 Jan 2022 20:55:49: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:55:53: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 20:55:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:55:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:55:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:55:55: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.10_peaks.xls INFO @ Sat, 15 Jan 2022 20:55:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.10_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 20:55:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.10_summits.bed INFO @ Sat, 15 Jan 2022 20:55:55: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (2363 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:55:58: 1000000 INFO @ Sat, 15 Jan 2022 20:56:03: 2000000 INFO @ Sat, 15 Jan 2022 20:56:07: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:56:11: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:56:16: 5000000 INFO @ Sat, 15 Jan 2022 20:56:19: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 20:56:19: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 20:56:19: #1 total tags in treatment: 2814499 INFO @ Sat, 15 Jan 2022 20:56:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:19: #1 tags after filtering in treatment: 1884184 INFO @ Sat, 15 Jan 2022 20:56:19: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 20:56:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:19: #2 number of paired peaks: 154 WARNING @ Sat, 15 Jan 2022 20:56:19: Fewer paired peaks (154) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 154 pairs to build model! INFO @ Sat, 15 Jan 2022 20:56:19: start model_add_line... INFO @ Sat, 15 Jan 2022 20:56:20: start X-correlation... INFO @ Sat, 15 Jan 2022 20:56:20: end of X-cor INFO @ Sat, 15 Jan 2022 20:56:20: #2 finished! INFO @ Sat, 15 Jan 2022 20:56:20: #2 predicted fragment length is 149 bps INFO @ Sat, 15 Jan 2022 20:56:20: #2 alternative fragment length(s) may be 149 bps INFO @ Sat, 15 Jan 2022 20:56:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.20_model.r INFO @ Sat, 15 Jan 2022 20:56:20: #3 Call peaks... INFO @ Sat, 15 Jan 2022 20:56:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 20:56:24: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 20:56:26: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.20_peaks.xls INFO @ Sat, 15 Jan 2022 20:56:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.20_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 20:56:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX10468556/SRX10468556.20_summits.bed INFO @ Sat, 15 Jan 2022 20:56:26: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (1277 records, 4 fields): 3 millis CompletedMACS2peakCalling