Job ID = 14521313 SRX = SRX10468542 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6332194 spots for SRR14094853/SRR14094853.sra Written 6332194 spots for SRR14094853/SRR14094853.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 6332194 reads; of these: 6332194 (100.00%) were paired; of these: 860979 (13.60%) aligned concordantly 0 times 4732942 (74.74%) aligned concordantly exactly 1 time 738273 (11.66%) aligned concordantly >1 times ---- 860979 pairs aligned concordantly 0 times; of these: 59936 (6.96%) aligned discordantly 1 time ---- 801043 pairs aligned 0 times concordantly or discordantly; of these: 1602086 mates make up the pairs; of these: 1464637 (91.42%) aligned 0 times 93044 (5.81%) aligned exactly 1 time 44405 (2.77%) aligned >1 times 88.43% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 333415 / 5493506 = 0.0607 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:55:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:55:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:55:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:55:09: 1000000 INFO @ Sat, 15 Jan 2022 20:55:13: 2000000 INFO @ Sat, 15 Jan 2022 20:55:17: 3000000 INFO @ Sat, 15 Jan 2022 20:55:22: 4000000 INFO @ Sat, 15 Jan 2022 20:55:26: 5000000 INFO @ Sat, 15 Jan 2022 20:55:30: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:55:34: 7000000 INFO @ Sat, 15 Jan 2022 20:55:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:55:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:55:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:55:39: 8000000 INFO @ Sat, 15 Jan 2022 20:55:39: 1000000 INFO @ Sat, 15 Jan 2022 20:55:43: 9000000 INFO @ Sat, 15 Jan 2022 20:55:43: 2000000 INFO @ Sat, 15 Jan 2022 20:55:48: 10000000 INFO @ Sat, 15 Jan 2022 20:55:48: 3000000 INFO @ Sat, 15 Jan 2022 20:55:50: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 20:55:50: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 20:55:50: #1 total tags in treatment: 5138689 INFO @ Sat, 15 Jan 2022 20:55:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:55:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:55:50: #1 tags after filtering in treatment: 4298260 INFO @ Sat, 15 Jan 2022 20:55:50: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:55:50: #1 finished! INFO @ Sat, 15 Jan 2022 20:55:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:55:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:55:51: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:55:51: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:55:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:55:52: 4000000 INFO @ Sat, 15 Jan 2022 20:55:57: 5000000 INFO @ Sat, 15 Jan 2022 20:56:01: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:56:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:56:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:56:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:56:05: 7000000 INFO @ Sat, 15 Jan 2022 20:56:09: 1000000 INFO @ Sat, 15 Jan 2022 20:56:09: 8000000 INFO @ Sat, 15 Jan 2022 20:56:13: 2000000 INFO @ Sat, 15 Jan 2022 20:56:14: 9000000 INFO @ Sat, 15 Jan 2022 20:56:18: 3000000 INFO @ Sat, 15 Jan 2022 20:56:19: 10000000 INFO @ Sat, 15 Jan 2022 20:56:21: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 20:56:21: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 20:56:21: #1 total tags in treatment: 5138689 INFO @ Sat, 15 Jan 2022 20:56:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:21: #1 tags after filtering in treatment: 4298260 INFO @ Sat, 15 Jan 2022 20:56:21: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:56:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:21: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:56:21: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:56:22: 4000000 INFO @ Sat, 15 Jan 2022 20:56:26: 5000000 INFO @ Sat, 15 Jan 2022 20:56:30: 6000000 INFO @ Sat, 15 Jan 2022 20:56:34: 7000000 INFO @ Sat, 15 Jan 2022 20:56:39: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:56:43: 9000000 INFO @ Sat, 15 Jan 2022 20:56:47: 10000000 INFO @ Sat, 15 Jan 2022 20:56:49: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 20:56:49: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 20:56:49: #1 total tags in treatment: 5138689 INFO @ Sat, 15 Jan 2022 20:56:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:56:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:56:49: #1 tags after filtering in treatment: 4298260 INFO @ Sat, 15 Jan 2022 20:56:49: #1 Redundant rate of treatment: 0.16 INFO @ Sat, 15 Jan 2022 20:56:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:56:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:56:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:56:50: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:56:50: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:56:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468542/SRX10468542.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。