Job ID = 14521517 SRX = SRX10468494 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 2767130 spots for SRR14094805/SRR14094805.sra Written 2767130 spots for SRR14094805/SRR14094805.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:00 2767130 reads; of these: 2767130 (100.00%) were paired; of these: 421863 (15.25%) aligned concordantly 0 times 2056418 (74.32%) aligned concordantly exactly 1 time 288849 (10.44%) aligned concordantly >1 times ---- 421863 pairs aligned concordantly 0 times; of these: 26718 (6.33%) aligned discordantly 1 time ---- 395145 pairs aligned 0 times concordantly or discordantly; of these: 790290 mates make up the pairs; of these: 737667 (93.34%) aligned 0 times 35180 (4.45%) aligned exactly 1 time 17443 (2.21%) aligned >1 times 86.67% overall alignment rate Time searching: 00:02:00 Overall time: 00:02:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 74078 / 2358114 = 0.0314 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:51: 1000000 INFO @ Sat, 15 Jan 2022 21:09:58: 2000000 INFO @ Sat, 15 Jan 2022 21:10:05: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:14: 4000000 INFO @ Sat, 15 Jan 2022 21:10:19: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:10:19: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:10:19: #1 total tags in treatment: 2271424 INFO @ Sat, 15 Jan 2022 21:10:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:10:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:10:19: #1 tags after filtering in treatment: 2055302 INFO @ Sat, 15 Jan 2022 21:10:19: #1 Redundant rate of treatment: 0.10 INFO @ Sat, 15 Jan 2022 21:10:19: #1 finished! INFO @ Sat, 15 Jan 2022 21:10:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:10:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:10:19: #2 number of paired peaks: 55 WARNING @ Sat, 15 Jan 2022 21:10:19: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:10:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:10:20: 1000000 INFO @ Sat, 15 Jan 2022 21:10:27: 2000000 INFO @ Sat, 15 Jan 2022 21:10:34: 3000000 INFO @ Sat, 15 Jan 2022 21:10:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:44: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:10:44: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:10:44: #1 total tags in treatment: 2271424 INFO @ Sat, 15 Jan 2022 21:10:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:10:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:10:44: #1 tags after filtering in treatment: 2055302 INFO @ Sat, 15 Jan 2022 21:10:44: #1 Redundant rate of treatment: 0.10 INFO @ Sat, 15 Jan 2022 21:10:44: #1 finished! INFO @ Sat, 15 Jan 2022 21:10:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:10:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:10:45: #2 number of paired peaks: 55 WARNING @ Sat, 15 Jan 2022 21:10:45: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:10:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:10:50: 1000000 INFO @ Sat, 15 Jan 2022 21:10:57: 2000000 INFO @ Sat, 15 Jan 2022 21:11:04: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:11:12: 4000000 INFO @ Sat, 15 Jan 2022 21:11:16: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:11:16: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:11:16: #1 total tags in treatment: 2271424 INFO @ Sat, 15 Jan 2022 21:11:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:17: #1 tags after filtering in treatment: 2055302 INFO @ Sat, 15 Jan 2022 21:11:17: #1 Redundant rate of treatment: 0.10 INFO @ Sat, 15 Jan 2022 21:11:17: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:17: #2 number of paired peaks: 55 WARNING @ Sat, 15 Jan 2022 21:11:17: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468494/SRX10468494.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。