Job ID = 14521484 SRX = SRX10468484 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6791872 spots for SRR14094795/SRR14094795.sra Written 6791872 spots for SRR14094795/SRR14094795.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:20 6791872 reads; of these: 6791872 (100.00%) were paired; of these: 874687 (12.88%) aligned concordantly 0 times 4784174 (70.44%) aligned concordantly exactly 1 time 1133011 (16.68%) aligned concordantly >1 times ---- 874687 pairs aligned concordantly 0 times; of these: 69251 (7.92%) aligned discordantly 1 time ---- 805436 pairs aligned 0 times concordantly or discordantly; of these: 1610872 mates make up the pairs; of these: 1439610 (89.37%) aligned 0 times 104268 (6.47%) aligned exactly 1 time 66994 (4.16%) aligned >1 times 89.40% overall alignment rate Time searching: 00:02:20 Overall time: 00:02:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 221732 / 5922543 = 0.0374 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:35: 1000000 INFO @ Sat, 15 Jan 2022 21:09:41: 2000000 INFO @ Sat, 15 Jan 2022 21:09:46: 3000000 INFO @ Sat, 15 Jan 2022 21:09:52: 4000000 INFO @ Sat, 15 Jan 2022 21:09:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:02: 6000000 INFO @ Sat, 15 Jan 2022 21:10:05: 1000000 INFO @ Sat, 15 Jan 2022 21:10:08: 7000000 INFO @ Sat, 15 Jan 2022 21:10:09: 2000000 INFO @ Sat, 15 Jan 2022 21:10:13: 8000000 INFO @ Sat, 15 Jan 2022 21:10:14: 3000000 INFO @ Sat, 15 Jan 2022 21:10:19: 4000000 INFO @ Sat, 15 Jan 2022 21:10:19: 9000000 INFO @ Sat, 15 Jan 2022 21:10:23: 5000000 INFO @ Sat, 15 Jan 2022 21:10:24: 10000000 INFO @ Sat, 15 Jan 2022 21:10:28: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:29: 11000000 INFO @ Sat, 15 Jan 2022 21:10:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:32: 7000000 INFO @ Sat, 15 Jan 2022 21:10:33: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:10:33: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:10:33: #1 total tags in treatment: 5695551 INFO @ Sat, 15 Jan 2022 21:10:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:10:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:10:33: #1 tags after filtering in treatment: 4500080 INFO @ Sat, 15 Jan 2022 21:10:33: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 21:10:33: #1 finished! INFO @ Sat, 15 Jan 2022 21:10:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:10:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:10:33: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 21:10:33: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:10:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:10:35: 1000000 INFO @ Sat, 15 Jan 2022 21:10:37: 8000000 INFO @ Sat, 15 Jan 2022 21:10:39: 2000000 INFO @ Sat, 15 Jan 2022 21:10:41: 9000000 INFO @ Sat, 15 Jan 2022 21:10:44: 3000000 INFO @ Sat, 15 Jan 2022 21:10:46: 10000000 INFO @ Sat, 15 Jan 2022 21:10:48: 4000000 INFO @ Sat, 15 Jan 2022 21:10:50: 11000000 INFO @ Sat, 15 Jan 2022 21:10:53: 5000000 INFO @ Sat, 15 Jan 2022 21:10:53: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:10:53: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:10:53: #1 total tags in treatment: 5695551 INFO @ Sat, 15 Jan 2022 21:10:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:10:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:10:54: #1 tags after filtering in treatment: 4500080 INFO @ Sat, 15 Jan 2022 21:10:54: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 21:10:54: #1 finished! INFO @ Sat, 15 Jan 2022 21:10:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:10:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:10:54: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 21:10:54: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:10:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:10:57: 6000000 INFO @ Sat, 15 Jan 2022 21:11:01: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:11:05: 8000000 INFO @ Sat, 15 Jan 2022 21:11:09: 9000000 INFO @ Sat, 15 Jan 2022 21:11:13: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:11:17: 11000000 INFO @ Sat, 15 Jan 2022 21:11:20: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:11:20: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:11:20: #1 total tags in treatment: 5695551 INFO @ Sat, 15 Jan 2022 21:11:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:20: #1 tags after filtering in treatment: 4500080 INFO @ Sat, 15 Jan 2022 21:11:20: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 21:11:20: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:20: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 21:11:20: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468484/SRX10468484.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling