Job ID = 14521448 SRX = SRX10468472 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4438138 spots for SRR14094884/SRR14094884.sra Written 4438138 spots for SRR14094884/SRR14094884.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:21 4438138 reads; of these: 4438138 (100.00%) were paired; of these: 1483527 (33.43%) aligned concordantly 0 times 2482257 (55.93%) aligned concordantly exactly 1 time 472354 (10.64%) aligned concordantly >1 times ---- 1483527 pairs aligned concordantly 0 times; of these: 19459 (1.31%) aligned discordantly 1 time ---- 1464068 pairs aligned 0 times concordantly or discordantly; of these: 2928136 mates make up the pairs; of these: 2845704 (97.18%) aligned 0 times 58375 (1.99%) aligned exactly 1 time 24057 (0.82%) aligned >1 times 67.94% overall alignment rate Time searching: 00:01:21 Overall time: 00:01:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 108769 / 2956225 = 0.0368 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:02:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:02:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:02:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:02:20: 1000000 INFO @ Sat, 15 Jan 2022 21:02:24: 2000000 INFO @ Sat, 15 Jan 2022 21:02:29: 3000000 INFO @ Sat, 15 Jan 2022 21:02:34: 4000000 INFO @ Sat, 15 Jan 2022 21:02:38: 5000000 INFO @ Sat, 15 Jan 2022 21:02:42: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:02:42: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:02:42: #1 total tags in treatment: 2845888 INFO @ Sat, 15 Jan 2022 21:02:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:02:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:02:42: #1 tags after filtering in treatment: 2455830 INFO @ Sat, 15 Jan 2022 21:02:42: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:02:42: #1 finished! INFO @ Sat, 15 Jan 2022 21:02:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:02:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:02:42: #2 number of paired peaks: 29 WARNING @ Sat, 15 Jan 2022 21:02:42: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:02:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:02:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:02:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:02:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:02:49: 1000000 INFO @ Sat, 15 Jan 2022 21:02:53: 2000000 INFO @ Sat, 15 Jan 2022 21:02:57: 3000000 INFO @ Sat, 15 Jan 2022 21:03:01: 4000000 INFO @ Sat, 15 Jan 2022 21:03:05: 5000000 INFO @ Sat, 15 Jan 2022 21:03:08: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:03:08: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:03:08: #1 total tags in treatment: 2845888 INFO @ Sat, 15 Jan 2022 21:03:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:03:08: #1 tags after filtering in treatment: 2455830 INFO @ Sat, 15 Jan 2022 21:03:08: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:03:08: #1 finished! INFO @ Sat, 15 Jan 2022 21:03:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:03:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:03:08: #2 number of paired peaks: 29 WARNING @ Sat, 15 Jan 2022 21:03:08: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:03:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:03:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:03:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:03:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:03:20: 1000000 INFO @ Sat, 15 Jan 2022 21:03:24: 2000000 INFO @ Sat, 15 Jan 2022 21:03:28: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:03:33: 4000000 INFO @ Sat, 15 Jan 2022 21:03:37: 5000000 INFO @ Sat, 15 Jan 2022 21:03:41: #1 tag size is determined as 25 bps INFO @ Sat, 15 Jan 2022 21:03:41: #1 tag size = 25 INFO @ Sat, 15 Jan 2022 21:03:41: #1 total tags in treatment: 2845888 INFO @ Sat, 15 Jan 2022 21:03:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:03:41: #1 tags after filtering in treatment: 2455830 INFO @ Sat, 15 Jan 2022 21:03:41: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:03:41: #1 finished! INFO @ Sat, 15 Jan 2022 21:03:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:03:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:03:41: #2 number of paired peaks: 29 WARNING @ Sat, 15 Jan 2022 21:03:41: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:03:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10468472/SRX10468472.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。