Job ID = 14521785 SRX = SRX10459800 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10921525 spots for SRR14085599/SRR14085599.sra Written 10921525 spots for SRR14085599/SRR14085599.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:26 10921525 reads; of these: 10921525 (100.00%) were paired; of these: 1432954 (13.12%) aligned concordantly 0 times 8476800 (77.62%) aligned concordantly exactly 1 time 1011771 (9.26%) aligned concordantly >1 times ---- 1432954 pairs aligned concordantly 0 times; of these: 138798 (9.69%) aligned discordantly 1 time ---- 1294156 pairs aligned 0 times concordantly or discordantly; of these: 2588312 mates make up the pairs; of these: 2316133 (89.48%) aligned 0 times 161808 (6.25%) aligned exactly 1 time 110371 (4.26%) aligned >1 times 89.40% overall alignment rate Time searching: 00:07:26 Overall time: 00:07:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1651473 / 9609997 = 0.1718 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:49:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:49:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:49:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:49:58: 1000000 INFO @ Sat, 15 Jan 2022 21:50:03: 2000000 INFO @ Sat, 15 Jan 2022 21:50:09: 3000000 INFO @ Sat, 15 Jan 2022 21:50:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:50:20: 5000000 INFO @ Sat, 15 Jan 2022 21:50:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:50:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:50:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:50:25: 6000000 INFO @ Sat, 15 Jan 2022 21:50:27: 1000000 INFO @ Sat, 15 Jan 2022 21:50:31: 7000000 INFO @ Sat, 15 Jan 2022 21:50:33: 2000000 INFO @ Sat, 15 Jan 2022 21:50:37: 8000000 INFO @ Sat, 15 Jan 2022 21:50:39: 3000000 INFO @ Sat, 15 Jan 2022 21:50:43: 9000000 INFO @ Sat, 15 Jan 2022 21:50:44: 4000000 INFO @ Sat, 15 Jan 2022 21:50:49: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:50:50: 5000000 INFO @ Sat, 15 Jan 2022 21:50:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:50:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:50:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:50:55: 11000000 INFO @ Sat, 15 Jan 2022 21:50:57: 6000000 INFO @ Sat, 15 Jan 2022 21:50:58: 1000000 INFO @ Sat, 15 Jan 2022 21:51:01: 12000000 INFO @ Sat, 15 Jan 2022 21:51:03: 7000000 INFO @ Sat, 15 Jan 2022 21:51:04: 2000000 INFO @ Sat, 15 Jan 2022 21:51:07: 13000000 INFO @ Sat, 15 Jan 2022 21:51:09: 8000000 INFO @ Sat, 15 Jan 2022 21:51:11: 3000000 INFO @ Sat, 15 Jan 2022 21:51:14: 14000000 INFO @ Sat, 15 Jan 2022 21:51:15: 9000000 INFO @ Sat, 15 Jan 2022 21:51:17: 4000000 INFO @ Sat, 15 Jan 2022 21:51:20: 15000000 INFO @ Sat, 15 Jan 2022 21:51:21: 10000000 INFO @ Sat, 15 Jan 2022 21:51:23: 5000000 INFO @ Sat, 15 Jan 2022 21:51:26: 16000000 INFO @ Sat, 15 Jan 2022 21:51:28: 11000000 INFO @ Sat, 15 Jan 2022 21:51:28: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:51:28: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:51:28: #1 total tags in treatment: 7848679 INFO @ Sat, 15 Jan 2022 21:51:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:51:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:51:28: #1 tags after filtering in treatment: 5691696 INFO @ Sat, 15 Jan 2022 21:51:28: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 21:51:28: #1 finished! INFO @ Sat, 15 Jan 2022 21:51:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:51:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:51:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:51:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:51:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:51:29: 6000000 INFO @ Sat, 15 Jan 2022 21:51:34: 12000000 INFO @ Sat, 15 Jan 2022 21:51:36: 7000000 INFO @ Sat, 15 Jan 2022 21:51:40: 13000000 INFO @ Sat, 15 Jan 2022 21:51:42: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:51:46: 14000000 INFO @ Sat, 15 Jan 2022 21:51:48: 9000000 INFO @ Sat, 15 Jan 2022 21:51:53: 15000000 INFO @ Sat, 15 Jan 2022 21:51:54: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:51:59: 16000000 INFO @ Sat, 15 Jan 2022 21:52:00: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:52:00: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:52:00: #1 total tags in treatment: 7848679 INFO @ Sat, 15 Jan 2022 21:52:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:00: 11000000 INFO @ Sat, 15 Jan 2022 21:52:00: #1 tags after filtering in treatment: 5691696 INFO @ Sat, 15 Jan 2022 21:52:00: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 21:52:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:52:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:52:06: 12000000 INFO @ Sat, 15 Jan 2022 21:52:12: 13000000 INFO @ Sat, 15 Jan 2022 21:52:17: 14000000 INFO @ Sat, 15 Jan 2022 21:52:23: 15000000 INFO @ Sat, 15 Jan 2022 21:52:29: 16000000 INFO @ Sat, 15 Jan 2022 21:52:30: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 21:52:30: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 21:52:30: #1 total tags in treatment: 7848679 INFO @ Sat, 15 Jan 2022 21:52:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:30: #1 tags after filtering in treatment: 5691696 INFO @ Sat, 15 Jan 2022 21:52:30: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 21:52:30: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:52:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459800/SRX10459800.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling