Job ID = 14521953 SRX = SRX10459796 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10571808 spots for SRR14085595/SRR14085595.sra Written 10571808 spots for SRR14085595/SRR14085595.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:36 10571808 reads; of these: 10571808 (100.00%) were paired; of these: 2100549 (19.87%) aligned concordantly 0 times 6661798 (63.01%) aligned concordantly exactly 1 time 1809461 (17.12%) aligned concordantly >1 times ---- 2100549 pairs aligned concordantly 0 times; of these: 106177 (5.05%) aligned discordantly 1 time ---- 1994372 pairs aligned 0 times concordantly or discordantly; of these: 3988744 mates make up the pairs; of these: 3671145 (92.04%) aligned 0 times 188708 (4.73%) aligned exactly 1 time 128891 (3.23%) aligned >1 times 82.64% overall alignment rate Time searching: 00:06:36 Overall time: 00:06:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1383290 / 8563197 = 0.1615 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:05:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:05:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:05:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:06:01: 1000000 INFO @ Sat, 15 Jan 2022 22:06:06: 2000000 INFO @ Sat, 15 Jan 2022 22:06:12: 3000000 INFO @ Sat, 15 Jan 2022 22:06:17: 4000000 INFO @ Sat, 15 Jan 2022 22:06:22: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:06:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:06:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:06:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:06:28: 6000000 INFO @ Sat, 15 Jan 2022 22:06:32: 1000000 INFO @ Sat, 15 Jan 2022 22:06:34: 7000000 INFO @ Sat, 15 Jan 2022 22:06:38: 2000000 INFO @ Sat, 15 Jan 2022 22:06:40: 8000000 INFO @ Sat, 15 Jan 2022 22:06:44: 3000000 INFO @ Sat, 15 Jan 2022 22:06:46: 9000000 INFO @ Sat, 15 Jan 2022 22:06:50: 4000000 INFO @ Sat, 15 Jan 2022 22:06:52: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:06:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:06:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:06:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:06:56: 5000000 INFO @ Sat, 15 Jan 2022 22:06:58: 11000000 INFO @ Sat, 15 Jan 2022 22:07:03: 6000000 INFO @ Sat, 15 Jan 2022 22:07:03: 1000000 INFO @ Sat, 15 Jan 2022 22:07:04: 12000000 INFO @ Sat, 15 Jan 2022 22:07:09: 7000000 INFO @ Sat, 15 Jan 2022 22:07:10: 2000000 INFO @ Sat, 15 Jan 2022 22:07:11: 13000000 INFO @ Sat, 15 Jan 2022 22:07:15: 8000000 INFO @ Sat, 15 Jan 2022 22:07:17: 3000000 INFO @ Sat, 15 Jan 2022 22:07:17: 14000000 INFO @ Sat, 15 Jan 2022 22:07:21: 9000000 INFO @ Sat, 15 Jan 2022 22:07:21: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:07:21: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:07:21: #1 total tags in treatment: 7096200 INFO @ Sat, 15 Jan 2022 22:07:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:07:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:07:22: #1 tags after filtering in treatment: 4960035 INFO @ Sat, 15 Jan 2022 22:07:22: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 22:07:22: #1 finished! INFO @ Sat, 15 Jan 2022 22:07:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:07:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:07:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:07:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:07:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:07:23: 4000000 INFO @ Sat, 15 Jan 2022 22:07:27: 10000000 INFO @ Sat, 15 Jan 2022 22:07:29: 5000000 INFO @ Sat, 15 Jan 2022 22:07:33: 11000000 INFO @ Sat, 15 Jan 2022 22:07:36: 6000000 INFO @ Sat, 15 Jan 2022 22:07:39: 12000000 INFO @ Sat, 15 Jan 2022 22:07:42: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:07:45: 13000000 INFO @ Sat, 15 Jan 2022 22:07:48: 8000000 INFO @ Sat, 15 Jan 2022 22:07:51: 14000000 INFO @ Sat, 15 Jan 2022 22:07:54: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:07:56: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:07:56: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:07:56: #1 total tags in treatment: 7096200 INFO @ Sat, 15 Jan 2022 22:07:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:07:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:07:56: #1 tags after filtering in treatment: 4960035 INFO @ Sat, 15 Jan 2022 22:07:56: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 22:07:56: #1 finished! INFO @ Sat, 15 Jan 2022 22:07:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:07:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:07:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:07:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:07:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:08:00: 10000000 INFO @ Sat, 15 Jan 2022 22:08:06: 11000000 INFO @ Sat, 15 Jan 2022 22:08:12: 12000000 INFO @ Sat, 15 Jan 2022 22:08:18: 13000000 INFO @ Sat, 15 Jan 2022 22:08:23: 14000000 INFO @ Sat, 15 Jan 2022 22:08:28: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 22:08:28: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 22:08:28: #1 total tags in treatment: 7096200 INFO @ Sat, 15 Jan 2022 22:08:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:08:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:08:28: #1 tags after filtering in treatment: 4960035 INFO @ Sat, 15 Jan 2022 22:08:28: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 22:08:28: #1 finished! INFO @ Sat, 15 Jan 2022 22:08:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:08:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:08:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:08:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:08:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX10459796/SRX10459796.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling